During a survey of yeasts occurring in exudates of various tree species in the Pacific Northwest of North America, 24 strains of an imperfect yeast were isolated in a wide geographic area, mainly from species of the genus Populus. The isolates were studied by traditional as well as molecular methods, and the results revealed a new species of the genus Candida. The new species is named Candida populi, because its principal habitat is in exudates of Populus species. C. populi resembles Candida molischiana but differs from this species in habitat, guanine-plus-cytosine content of the nuclear deoxyribonucleic acid, maximum growth temperature, and ability to assimilate several carbon compounds. The type strain of C. populi is strain UCD-FST 68-675B (= CBS 7351 = ATCC 64933).