In accordance with Recommendation 30b of the International Code of Nomenclature of Bacteria, minimal standards are proposed for the genus Staphylococcus and the description of newly recognized species in this genus. Assignment of a strain to the genus Staphylococcus requires that it is a Gram-positive coccus that forms clusters, produces catalase, has an appropriate cell wall structure (including peptidoglycan type and teichoic acid presence) and G+C content of DNA in a range of 30-40 mol%. The recommended minimal standards for describing a new Staphylococcus species are based on the results of phenotypic and genomic studies of at least five independently isolated strains. They include colony morphology and the results of the following conventional tests: pigment production, growth requirements, fermentative and oxidative activity on carbohydrates, novobiocin susceptibility, enzymic activities (nitrate reductase, alkaline phosphatase, arginine dihydrolase, ornithine decarboxylase, urease, cytochrome oxidase, staphylocoagulase in rabbit plasma, heat-stable nuclease, amidases, oxidases, clumping factor, and haemolytic activity on sheep or bovine blood agar). DNA-DNA hybridization experiments may distinguish species when the difference between the binding in the homologous reaction and the binding in the heterologous reaction expressed as a percentage is less than 70%. In addition, rRNA signature sequence criteria, ribotyping characterization of the nomenclature type strain and other strains of the species, and reference strains of other species is recommended to describe the strains of the new species with sets of genetic attributes and reveal possible grouping errors. This proposal has been endorsed by the members of the Subcommittee on the taxonomy of staphylococci and streptococci of the International Committee on Systematic Bacteriology.
The Request for an Opinion by Le Minor and Popoff 1987, proposing designation of ‘Salmonella enterica’ (ex Kauffmann and Edwards 1952) Le Minor and Popoff 1987 as the type and only species of the genus Salmonella Lignieres 1900 (Approved Lists 1980), has not been positively decided upon by the Judicial Commission. However, many bacteriologists use the name ‘Salmonella enterica’. To avoid further confusion, it is requested to reject the name Salmonella choleraesuis (Smith 1894) Weldin 1927 (Approved Lists 1980), to recognize the species Salmonella enterica, to conserve the name Salmoneella typhi (Schroeter 1886) Warren and Scott 1930 (Approved Lists 1980), and to emend the genus Salmonella with the establishment of a neotype species, Salmonella enterica.
It is proposed that the type strain of Streptomyces rameus Shibata 1959 is IFO 3782 (= No. 43797), which is the originally designated type strain, and not ATCC 21273 as given in the Approved Lists. The level of DNA relatedness between IFO 3782 and ATCC 21273 is 30%, indicating that they are different species.