A novel filamentous, endospore-forming, thermophilic and moderately halophilic bacterium designated strain Nari2AT was isolated from soil collected from an Algerian salt lake, Chott Melghir. The novel isolate was Gram-staining-positive, aerobic, catalase-negative and oxidase-positive. Optimum growth occurred at 50–55 °C, 7–10 % (w/v) NaCl and pH 7–8. The strain exhibited 95.4, 95.4 and 95.2 % 16S rRNA gene sequence similarity to Thalassobacillus devorans G19.1T, Sediminibacillus halophilus EN8dT and Virgibacillus kekensis YIM-kkny16T, respectively. The major menaquinone was MK-7. The polar lipid profile consisted of phosphatidylglycerol, diphosphatidylglycerol, three unknown phosphoglycolipids and two unknown phospholipids. The predominant cellular fatty acids were iso-C15 : 0 and iso-C17 : 0. The DNA G+C content was 41.9 mol%. Based on the phenotypic, chemotaxonomic and phylogenetic data, strain Nari2AT is considered to represent a novel species of a new genus in the family Bacillaceae , order Bacillales , for which the name Melghiribacillus thermohalophilus gen. nov., sp. nov. is proposed. The type strain of Melghiribacillus thermohalophilus is Nari2AT ( = DSM 25894T = CCUG 62543T).
A Gram-stain-positive, spore-forming, obligately anaerobic bacterium, designated LAM201T, was isolated from sediment samples from an alkaline-saline lake located in Daqing oilfield, Daqing City, PR China. Cells of strain LAM201T were non-motile and straight or spiral rod-shapes. Strain LAM201T was able to utilize glucose, fructose, maltose, trehalose and sorbitol as the sole carbon source. Acetic acid, ethanol, iso-butanoic acid and iso-valeric acid were the main products of glucose fermentation. The major fatty acids of LAM201T were C16 : 0 (26.7 %) and C18 : 0 (11.2 %). The main polar lipids were four unknown glycolipids and five unknown phospholipids. The predominant cell-wall sugars were ribose and galactose. The cell-wall peptidoglycan of strain LAM201T contained alanine, glycine, glutamic acid and aspartic acid. Sodium sulfite was used as the electron acceptor. The G+C content of the genomic DNA was 32±0.8 mol%, as determined by the T m method. Analysis of the 16S rRNA gene sequence indicated that the isolate belonged to the genus Romboutsia and was most closely related to Romboutsia lituseburensis DSM 797T and Romboutsia ilealis CRIBT with 97.3 % and 97.2 % similarities, respectively. The DNA–DNA hybridization values between strain LAM201T and the two reference strains were 37 % and 31 %, respectively. On the basis of its phenotypic, phylogenetic and chemotaxonomic characteristics, strain LAM201T is suggested to represent a novel species within the genus Romboutsia , for which the name Romboutsia sedimentorum sp. nov. is proposed. The type strain is LAM201T ( = ACCC 00717T = JCM 19607T).
Two Gram-stain-positive, endospore-forming lactic acid bacteria, designated BK92T and BK117-1T, were isolated from tree barks in Thailand. Cells were catalase-negative and facultatively anaerobic rods. 16S rRNA gene sequence analysis indicated that these strains belonged to the genus Sporolactobacillus . Strains BK92T and BK117-1T showed the highest 16S rRNA gene sequence similarity to Sporolactobacillus putidus QC81-06T with 97.7 % and 97.1 % similarity, respectively. Analysis of phylogenetic relationships based on 16S rRNA and gyrB gene sequencing revealed that the positions of strains BK92T and BK117-1T were clearly separated from all related species of the genus Sporolactobacillus . Strains BK92T and BK117-1T had low DNA–DNA relatedness between each other and also with S. putidus QC81-06T and Sporolactobacillus vineae SL153T. The DNA G+C content of strains BK92T and BK117-1T was 46.6 mol% and 47.4 mol%, respectively. The major fatty acids of strains BK92T and BK117-1T were anteiso-C17 : 0 and anteiso-C15 : 0. They contained meso-diaminopimelic acid in cell-wall peptidoglycan and had menaquinone with seven isoprene units (MK-7) as the predominant menaquinone. Based on evidence including phenotypic, genotypic and chemotaxonomic studies, strains BK92T and BK117-1T should be classified as representatives of novel species of the genus Sporolactobacillus , for which the names Sporolactobacillus shoreae sp. nov. and Sporolactobacillus spathodeae sp. nov. are proposed, respectively. The type strains are BK92T ( = JCM 19541T = LMG 28365T = PCU 336T = TISTR 2234T) and BK117-1T ( = JCM 19542T = LMG 28366T = PCU 337T = TISTR 2235T).
Two Gram-stain-positive, diesel oil-degrading, solvent-tolerant, aerobic, endospore-forming, rod-shaped bacteria were isolated from a contaminated laboratory plate. Based on 16S rRNA gene sequence analysis, strains JC228T and JC279 were identified as belonging to the genus Bacillus within the family Bacillaceae of the phylum Firmicutes and were found to be most closely related to Bacillus carboniphilus JCM 9731T (98.1 % 16S rRNA gene sequence similarity) and shared <96.0 % 16S rRNA gene sequence similarity with other members of the genus Bacillus . The DNA–DNA hybridization value between the two strains was 88±2 %. Strain JC228T showed 23.4±1 % reassociation (based on DNA–DNA hybridization) with B. carboniphilus LMG 18001T. The DNA G+C content of strains JC228T and JC279 was 39 and 38.4 mol%, respectively. Both strains were positive for catalase and oxidase activities, and negative for hydrolysis of starch and Tween 80. Strains JC228T and JC279 grew chemoorganoheterotrophically with optimum growth at pH 7 (range pH 7–9.5) and 35 °C (range 25–40 °C). Diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and an unidentified phospholipid (PL2) were the major polar lipids. Major cellular fatty acids were iso-C15 : 0, anteiso-C15 : 0, iso-C17 : 0 and C16 : 0. Whole-cell hydrolysates contained l-alanine, d-alanine, d-glutamic acid and meso-diaminopimelic acid. Both strains utilized diesel oil as sole carbon and energy source. The results of physiological, biochemical, chemotaxonomic and molecular analyses allowed clear differentiation of strains JC228T and JC279 from their closest phylogenetic neighbours. Therefore strains JC228T and JC279 represent a novel species of the genus Bacillus , for which the name Bacillus oleivorans sp. nov. is proposed. The type strain is JC228T ( = LMG 28084T = CCTCC AB 2013353T).