A polyphasic taxonomic approach was used to study strain 12116T, which was isolated from desert sand in Xinjiang, China. Cells of the isolate were Gram-negative, non-motile, rod-shaped and aerobic. MK-7 was the predominant respiratory menaquinone. The DNA G+C content was 46.3 mol%. A phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate was most closely related to the members of the genus Dyadobacter, with similarities ranging from 94.7 to 96.6 %. Therefore, on the basis of genotypic and phenotypic data, strain 12116T represents a novel species of the genus Dyadobacter, for which the name Dyadobacter alkalitolerans sp. nov. is proposed. The type strain is 12116T (=CCTCC AB 207176T =NRRL B-51268T).
A marine bacterium, designated IMCC3223T, was isolated from Antarctic coastal seawater and subjected to a polyphasic taxonomic investigation. Cells of the strain were Gram-negative, short to elongated rods that were orange-coloured but negative for flexirubin-type pigments, obligately aerobic, chemoheterotrophic and devoid of gliding or flagellar motility. On the basis of 16S rRNA gene sequence comparisons, the strain was closely related to members of the genus Lewinella (86.3–93.1 %) of the family ‘Saprospiraceae’ in the phylum Bacteroidetes; the most closely related species was Lewinella lutea (93.1 % similarity to the type strain). The DNA G+C content of the strain was 50.3 mol% and the major respiratory quinone was MK-7. The predominant cellular fatty acids were C16 : 1 ω7c/iso-C15 : 0 2-OH (39.4 %), iso-C15 : 0 (15.9 %), C17 : 1 ω7c (10.5 %) and C15 : 1 ω6c (8.9 %). 16S rRNA gene sequence analyses and the phenotypic and chemotaxonomic tests allowed the differentiation of strain IMCC3223T from all recognized species of the genus Lewinella. Therefore, strain IMCC3223T represents a novel species of the genus Lewinella, for which the name Lewinella antarctica sp. nov. is proposed. The type strain is IMCC3223T (=KCCM 42688T =NBRC 103142T).
A novel bacterial strain, designated Dae 13T, was isolated from sediment from a freshwater lake in Daejeon, South Korea, and was characterized taxonomically by using a polyphasic approach. The isolate was Gram-negative, aerobic, non-motile, non-spore-forming and rod-shaped. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate belonged to the genus Pedobacter in the family Sphingobacteriaceae but was clearly separate from established species of this genus. The 16S rRNA gene sequence similarities between strain Dae 13T and type strains of Pedobacter species with validly published names ranged from 91.6 to 97.5 %. The G+C content of the genomic DNA was 33.8 mol%. Chemotaxonomic data, i.e. the presence of MK-7 as the major menaquinone and iso-C15 : 0, C16 : 0 and summed feature 3 (iso-C15 : 0 2-OH and/or C16 : 1 ω7c) as the major fatty acids, supported the affiliation of strain Dae 13T to the genus Pedobacter. However, the results of physiological and biochemical tests allowed phenotypic differentiation of the isolate with respect to Pedobacter species with validly published names. Therefore, strain Dae 13T represents a novel species within the genus Pedobacter, for which the name Pedobacter daechungensis sp. nov. is proposed. The type strain is Dae 13T (=KCTC 12637T=LMG 23489T).
A Gram-negative, rod-shaped, non-motile and pink-pigmented bacterium, designated strain ZLB-3T, was isolated from a desert soil sample collected from Xinjiang Province, China, and characterized by using a polyphasic taxonomic approach. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate belongs to the phylum Bacteroidetes and is related to the genus Hymenobacter. 16S rRNA gene sequence similarities between strain ZLB-3T and the type strains of Hymenobacter species with validly published names ranged from 90.0 to 94.6 %. No other taxa in the phylum Bacteroidetes showed more than 90 % sequence similarity to the isolate. The strain contained MK-7 as the predominant menaquinone. The major fatty acids were iso-C15 : 0 (27.8 %), anteiso-C17 : 1 B/iso-C17 : 1 I (25.2 %), iso-C17 : 0 3-OH (9.6 %) and C16 : 1 ω7c/iso-C15 : 0 2-OH (8.5 %). Phosphatidylethanolamine, two unknown aminophospholipids, an unknown aminolipid, an unknown glycolipid and six unknown polar lipids were detected in the polar lipid profile. The DNA G+C content was 58.6 mol%. These chemotaxonomic data supported the affiliation of strain ZLB-3T to the genus Hymenobacter. However, the results of physiological and biochemical tests allowed phenotypic differentiation of the isolate from recognized Hymenobacter species. On the basis of the evidence presented, it is proposed that strain ZLB-3T represents a novel species, Hymenobacter deserti sp. nov. The type strain is ZLB-3T (=CCTCC AB 207171T =NRRL B-51267T).