A single Leptospira strain (designated Bejo-Iso9T) was isolated from a soil sample taken in Johor, Malaysia. The isolate showed motility and morphology typical of the genus Leptospira under dark-field microscopy. Cells were found to be 10–13 μm in length and 0.2 μm in diameter, with a wavelength of 0.5 μm and an amplitude of approximately 0.2 μm. Phenotypically, strain Bejo-Iso9T grew in Ellinghausen–McCullough–Johnson–Harris medium at 13, 30 and 37 °C, and also in the presence of 8-azaguanine. Serologically, strain Bejo-Iso9T produced titres towards several members of the Tarassovi serogroup, but was found to be serologically unique by cross-agglutinin absorption test and thus represented a novel serovar. The proposed name for this serovar is Malaysia. Phylogenetic analysis of 16S rRNA gene sequences placed this novel strain within the radiation of the genus Leptospira, with sequence similarities within the range 90.4–99.5% with respect to recognized Leptospira species. DNA–DNA hybridization against the three most closely related Leptospira species was used to confirm the results of the 16S rRNA gene sequence analysis. The G+C content of the genome of strain Bejo-Iso9T was 36.2 mol%. On the basis of phenotypic, serological and phylogenetic data, strain Bejo-Iso9T represents a novel species of the genus Leptospira, for which the name Leptospira kmetyi sp. nov. is proposed. The type strain is Bejo-Iso9T (=WHO LT1101T=KIT Bejo-Iso9T).
A Gram-positive-staining, spherical-shaped and faintly pink-pigmented bacterial strain, X-82T, was isolated from soil samples collected from a desert in Xinjiang, China. The organism was found to be resistant to UV radiation but sensitive to gamma radiation and desiccation. The optimum growth pH, NaCl concentration and temperature were pH 7.0, 0–1 % NaCl and 30 °C. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain X-82T is member of a novel species belonging to the genus Deinococcus, with Deinococcus hopiensis KR-140T as its closest relative (93.5 % similarity). The DNA G+C content (60 mol%), quinone type (MK-8), major cellular fatty acids (16 : 1ω7c, 16 : 0 and 17 : 1 iso ω9c), the presence of peptidoglycan with l-ornithine and the dominant polar lipid (phosphoglycolipids and glycolipids) support the affiliation of strain X-82T with the genus Deinococcus. A novel species is proposed, for which the name Deinococcus xinjiangensis sp. nov. is proposed, with the type strain X-82T (=CCTCC AB 207226T =NRRL B-51287T).