A Gram-positive, non-spore-forming actinomycete, designated strain CC 0387T, was isolated from a sediment sample from the Baltic Sea, Germany. Classification using a polyphasic approach and comparative 16S rRNA gene sequencing showed that strain CC 0387T belonged to the genus Promicromonospora and displayed more than 3 % 16S rRNA gene sequence divergence from all Promicromonospora species with validly published names. Strain CC 0387T did not produce aerial mycelium. Substrate mycelia were yellowish white to pale orange-yellow and fragmented into bacillary or coccoid elements. The cell wall contained lysine and alanine. Whole-cell hydrolysates contained galactose, glucose, rhamnose and ribose. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, an unknown phospholipid and an unknown glycolipid. The predominant menaquinone was MK-9(H4) (86 %). The G+C content of the DNA was 71.87 mol%. Based on morphological, chemotaxonomic, phenotypic and genetic characteristics, strain CC 0387T (=CCTCC AA208024T=DSM 21481T) represents a novel species, for which the name Promicromonospora flava sp. nov. is proposed.
Two Gram-positive, aerobic actinomycete strains, designated YIM 48771T and YIM 48782T, were isolated from virgin forest soil samples collected in Hunan Province, China. 16S rRNA gene sequence similarities of the two novel isolates ranged from 96.3 to 97.6 % with species of the genus Sphaerisporangium with validly published names but, in the tree based on 16S rRNA gene sequences, the isolates formed distinct phyletic lines. The level of 16S rRNA gene sequence similarity between the two novel isolates was 97.1 %. DNA–DNA hybridization of strains YIM 48771T and YIM 48782T with recognized species of the genus Sphaerisporangium revealed that the level of DNA–DNA relatedness was below 70 %. The DNA G+C contents of strains YIM 48771T and YIM 48782T were 67.1 and 71 mol%, respectively. Chemotaxonomic data [major menaquinone, MK-9(H4); major polar lipids, diphosphatidylglycerol, phosphatidylinositol mannoside, phosphatidylethanolamine and phosphoglycolipids; major fatty acids, iso-C16 : 0 and 10-methyl C17 : 0] supported the affiliation of the two isolates with the genus Sphaerisporangium. The results of DNA–DNA hybridization and physiological and biochemical tests allowed genotypic and phenotypic differentiation of the two isolates from recognized Sphaerisporangium species. Based on morphological, chemotaxonomical and phylogenetic data, strains YIM 48771T and YIM 48782T are considered to represent two novel species of the genus Sphaerisporangium, for which the names Sphaerisporangium flaviroseum sp. nov. (type strain, YIM 48771T=DSM 45170T=KCTC 19393T) and Sphaerisporangium album sp. nov. (type strain, YIM 48782T=DSM 45172T=CCTCC AA 208026T) are proposed.
An actinomycete strain, designated HBUM 170018T, was isolated from soil from Hebei Province, China, and subjected to a polyphasic taxonomic analysis. This study included morphological and physiological investigations and analyses concerning cell chemistry, genomic DNA G+C content, DNA–DNA hybridization and phylogeny. The data obtained were consistent with the assignment of strain HBUM 170018T to the genus Streptosporangium and were confirmed by the results of 16S rRNA gene sequence analysis. Strain HBUM 170018T can be differentiated from all Streptosporangium species with validly published names with reference to phenotypic characteristics, phylogenetic data and DNA–DNA hybridization results. On the basis of 16S rRNA gene sequencing, DNA–DNA hybridization and phenotypic data, strain HBUM 170018T represents a novel species of the genus Streptosporangium, for which the name Streptosporangium canum sp. nov. is proposed. The type strain is HBUM 170018T (=7177T =DSM 45034T =CGMCC 4.2126T).
A Gram-positive, non-motile, rod- or coccoid-shaped Microbacterium-like bacterium, designated strain DS-66T, was isolated from soil of Dokdo, Korea, and its exact taxonomic position was investigated by using a polyphasic approach. Strain DS-66T grew optimally at 30 °C and pH 6.5–7.0 in the presence of 0.5–1.0 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DS-66T belonged to the genus Microbacterium. Strain DS-66T had a peptidoglycan type based on B2β with partial substitution of glutamic acid by 3-hydroxy glutamic acid (Glu/Hyg–Gly–d-Orn), and galactose, rhamnose and ribose as whole-cell sugars. The acyl type was glycolyl. Strain DS-66T contained MK-13, MK-12 and MK-14 as predominant menaquinones and anteiso-C15 : 0, anteiso-C17 : 0, iso-C17 : 0 and iso-C16 : 0 as major fatty acids. Major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, an unidentified phospholipid and an unidentified glycolipid. The DNA G+C content was 69.9 mol%. Phylogenetic distinctiveness, DNA–DNA relatedness data and differential phenotypic properties demonstrated that strain DS-66T is distinguishable from recognized Microbacterium species. On the basis of the data presented, strain DS-66T is considered to represent a novel species of the genus Microbacterium, for which the name Microbacterium insulae sp. nov. is proposed. The type strain is DS-66T (=KCTC 19247T=CCUG 54523T).
The diversity of bacteria associated with inflamed mucosa was investigated by culturing ileal samples from TNFdeltaARE mice on a selective medium containing mucin. Among eight isolates, two strains (Mt1B3 and Mt1B8T) belonged to bacterial groups not yet cultured from the mouse intestine. Whereas strain Mt1B3 was identified as a member of the family Planococcaceae and is closely related to Sporosarcina species and Filibacter limicola DSM 13886T, strain Mt1B8T was a novel bacterium. Based on phylogenetic analysis, strain Mt1B8T is a member of the family Coriobacteriaceae. The closest relatives with validly published names were Asaccharobacter celatus, Adlercreutzia equolifaciens (<96 % similarity) and Eggerthella species (<92 %). With respect to Asaccharobacter celatus and Eggerthella, the phylogenetic position of strain Mt1B8T was confirmed at the chemotaxonomic level by Fourier-transform infrared spectroscopic analysis. The major fatty acid of strain Mt1B8T is C16 : 0 (23.9 %). Menaquinones were monomethylated. DNA–DNA relatedness between strain Mt1B8T and Asaccharobacter celatus DSM 18785T was 28 %. Strain Mt1B8T is a Gram-positive-staining rod that does not form spores and has a high DNA G+C content (64.2 mol%). Cells are aerotolerant but grow only under strictly anoxic conditions. They are sensitive to cefotaxime, clarithromycin, erythromycin, metronidazole, tetracycline, tobramycin and vancomycin. API and VITEK analysis showed the ability of strain Mt1B8T to convert a variety of amino acid derivatives. According to these findings, it is proposed to create a novel genus and species, Enterorhabdus mucosicola gen. nov., sp. nov., to accommodate strain Mt1B8T. The type strain of Enterorhabdus mucosicola is Mt1B8T (=DSM 19490T =CCUG 54980T).