An aerobic, Gram-stain-negative, rod-shaped bacterial isolate, strain NH36AT, was isolated from a sandy sediment sample from the South China Sea. Colonies of the isolate were dark orange on M2 agar. Optimal growth was observed at pH 7.0–8.5, 30 °C and in the presence of 0.5–4.0 % (w/v) NaCl. The major fatty acids were C15 : 0, iso-C15 : 0, anteiso-C15 : 0, iso-C15 : 1, iso-C15 : 0 3-OH, iso-C17 : 0 3-OH and summed feature 3 (comprising iso-C15 : 0 2-OH and/or C16 : 1 ω7c). The DNA G+C content was 38.9 mol%. 16S rRNA gene sequence analysis revealed that strain NH36AT was most closely related to members of the genus Arenibacter, exhibiting 94.3–96.2 % sequence similarity to the type strains of Arenibacter species. On the basis of phenotypic, chemotaxonomic and phylogenetic data, this organism should be classified as a representative of a novel species in the genus Arenibacter. The name Arenibacter nanhaiticus sp. nov. is proposed and the type strain is NH36AT (=LMG 24842T=CCTCC AB 208315T=MCCC 1A04137T).
A novel strain, designated 311-10T, isolated from soil of Xinjiang, China, was characterized by using a polyphasic taxonomic approach. The isolate was Gram-negative, aerobic, rod-shaped, non-motile, oxidase-negative and catalase-positive. The predominant menaquinone of strain 311-10T was MK-7 and the genomic DNA G+C content was 47.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate formed a cluster with the genera Pontibacter and [Effluviibacter] in the phylum ‘Bacteroidetes’, with sequence similarities of 93.9–95.6 %. Phylogenetic evidence and the results of phenotypic, genotypic and chemotaxonomic analyses support the reclassification of [Effluviibacter] roseus as Pontibacter roseus comb. nov. (type strain, SRC-1T=MTCC 7260T=DSM 17521T) and the establishment of a novel species, Pontibacter xinjiangensis sp. nov., with strain 311-10T (=CCTCC AB 207200T=NRRL B-51335T) as the type strain.
A Gram-staining-negative, rod-shaped marine bacterium, designated strain NH57NT, isolated from sandy sediment in the Mischief Reef of the South China Sea, was characterized based on its physiological and biochemical features, fatty acid profile and phylogenetic position. 16S rRNA gene sequence analysis revealed a clear affiliation with the family Flavobacteriaceae. Strain NH57NT showed the closest phylogenetic relationship with members of the genera Gaetbulibacter, Gelidibacter, Subsaxibacter, Subsaximicrobium and Yeosuana; levels of 16S rRNA gene sequence similarity between strain NH57NT and the type strains of related species ranged from 94.9 to 91.2 %. Cells of strain NH57NT were motile by gliding and grew on solid media as yellow colonies at 9–37 °C, pH 6.5–8.5 and in the presence of 0.5–4.0 % NaCl. The DNA G+C content was 32.7 mol% and the predominant fatty acids were iso-C15 : 1 (22.7 % of the total), iso-C15 : 0 (20.7 %), iso-C17 : 0 3-OH (9.5 %), iso-C16 : 0 3-OH (8.3 %), C15 : 0 (7.8 %) and iso-C15 : 0 3-OH (5.8 %). Based on the physiological and phylogenetic data, and on the fatty acid composition, strain NH57NT is considered to represent a novel species of a new genus in the family Flavobacteriaceae, for which the name Meridianimaribacter flavus gen. nov., sp. nov. is proposed. The type strain of Meridianimaribacter flavus is NH57NT (=CCTCC AB 208318T=LMG 24839T=MCCC 1A03544T).
A non-motile, rod-shaped bacterium, designated strain WPCB133T, was isolated from freshwater collected from the Woopo wetland (Republic of Korea). Cells were Gram-reaction-negative, aerobic and catalase- and oxidase-positive. The major fatty acids were iso-C15 : 0 and anteiso-C15 : 0. The strain contained MK-7 as the major isoprenoid quinone. The DNA G+C content was 47 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain WPCB133T forms an independent lineage within the genus Mucilaginibacter. Strain WPCB133T was distantly related to Mucilaginibacter kameinonensis SCKT (94.7 % sequence similarity), Mucilaginibacter paludis TPT56T (94.5 %) and Mucilaginibacter gracilis TPT18T (94.4 %). Phenotypic characteristics distinguished strain WPCB133T from members of the genus Mucilaginibacter. On the basis of evidence presented in this study, strain WPCB133T represents a novel species of the genus Mucilaginibacter, for which the name Mucilaginibacter rigui sp. nov. is proposed. The type strain is WPCB133T (=KCTC 12534T =NBRC 101115T). An emended description of the genus Mucilaginibacter is also proposed.
A Gram-negative, non-motile, non-spore-forming bacterial strain, S1-3T, was isolated from a tidal flat sediment on the west coast of Korea and its taxonomic position was investigated. Strain S1-3T grew optimally at 30 °C and in the presence of 2 % (w/v) NaCl. Strain S1-3T contained MK-7 as the predominant menaquinone and C16 : 1 ω7c and/or iso-C15 : 0 2-OH and iso-C15 : 0 as the major fatty acids. The DNA G+C content was 41.4 mol%. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain S1-3T fell within the clade comprising Algoriphagus species, clustering with Algoriphagus halophilus IMSNU 14013T, with which it exhibited 99.6 % 16S rRNA gene sequence similarity. The 16S rRNA gene sequence similarity between strain S1-3T and the type strains of other Algoriphagus species was 94.0–97.1 %. Differential phenotypic properties and phylogenetic and genetic distinctiveness of strain S1-3T demonstrated that this strain is distinguishable from the other Algoriphagus species as well as A. halophilus. On the basis of phenotypic, chemotaxonomic, phylogenetic and genetic data, strain S1-3T is considered to represent a novel species of the genus Algoriphagus, for which the name Algoriphagus lutimaris sp. nov. is proposed. The type strain is S1-3T (=KCTC 22630T =CCUG 57608T).
The taxonomic status of two novel heterotrophic, Gram-negative, gliding and yellow pigmented bacterial strains was established in this study. Phylogenetic analysis based on 16S rRNA gene sequencing revealed that the strains formed a distinct lineage within the genus Maribacter, a member of the family Flavobacteriaceae, with sequence similarities of 96.3–98.5 % to recognized species of the genus Maribacter. The maximum growth temperature of the strains was 35 °C and they required NaCl or seawater for growth. They hydrolysed aesculin and gelatin, reduced nitrates to nitrites and produced acid from carbohydrates. The DNA G+C contents of strains KMM 6025 and KMM 6046T were 36–37 mol%. On the basis of phenotypic, genotypic and phylogenetic characteristics, it is suggested that the new isolates represent a novel species of the genus Maribacter, for which the name Maribacter stanieri sp. nov. is proposed. The type strain is KMM 6046T (=KCTC 22023T=LMG 22581T).
Two Gram-staining-negative, rod-shaped, non-spore-forming bacterial strains, 1-2T and 1-4 were isolated from dry riverbed soil collected from the Xietongmen area of Tibet, China. On the basis of 16S rRNA gene sequence similarity, the novel strains were shown to belong to the genus Pedobacter, sharing <95 % sequence similarity with all recognized species of the genus Pedobacter. The major respiratory quinone was MK-7 and the predominant cellular fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH and summed feature 3 (comprising iso-C16 : 1 ω7c and/or C16 : 1 ω6c). The DNA G+C contents were 37.2–37.6 mol%. Chemotaxonomic data supported the affiliation of the two new isolates to the genus Pedobacter and the results of physiological and biochemical tests confirmed that the new strains differed significantly from the recognized species of the genus Pedobacter. Therefore, the new isolates represent a novel species within the genus Pedobacter, for which the name Pedobacter glucosidilyticus sp. nov. is proposed. The type strain is 1-2T (=CCTCC AB 206110T=KCTC 22438T).