Two strains of a novel yeast species were isolated from insect frass and a flower in Thailand and Brazil, respectively. The strain from Thailand was isolated from insect frass collected in Than-Tong waterfall, Nong Khai Province, whereas the strain from Brazil was recovered from a flower of Ipomoea sp. collected on the banks of the Paraguai River in the state of Mato Grosso do Sul. The sequences of the D1/D2 domains of the large subunit of the rDNA of both strains were identical. This novel species belonged to the Metschnikowia clade and was related to Metschnikowia lunata. No signs of sporulation were observed for the two strains on various culture media. The novel species, Candida golubevii sp. nov., is proposed to accommodate these isolates. The type strain is BCC 8332T (=CBS 11362T=NBRC 105679T).
Seven strains representing a novel yeast species belonging to the genus Cryptococcus were isolated from different substrates from Patagonia, Argentina, and The Netherlands. Three strains were isolated from a meltwater river draining from the Frias glacier at Mount Tronador situated in Nahuel Huapi National Park (Patagonia) and four were isolated from apple surfaces in Randwijk, The Netherlands. Analysis of the D1/D2 large-subunit rRNA gene and ITS region sequences indicated that these strains represent a single species that is distinct from other species of the Tremellales clade. The name Cryptococcus spencermartinsiae sp. nov. is proposed to accommodate these strains. The type strain is CRUB 1230T (=CBS 10760T =DBVPG 8010T).