A rod-shaped, non-spore-forming, non-motile, aerobic, oxidase and catalase-positive and radiation-resistant bacterium (designated strain K4.1T) was isolated from biofilm collected from a Finnish paper mill. The bacterium grew as pale pink colonies on oligotrophic medium at 12 to 50 °C (optimum 37 to 45 °C) and at pH 6 to 10.3. The DNA G+C content of the strain was 66.8 mol%. According to 16S rRNA gene sequence analysis, strain K4.1T was distantly related to the genus Deinococcus, sharing highest similarity with Deinococcus pimensis (90.0 %). In the phylogenetic tree, strain K4.1T formed a separate branch in the vicinity of the genus Deinococcus. The peptidoglycan type was A3β with l-Orn–Gly–Gly and the quinone system was determined to be MK-8. The polar lipid profile of strain K4.1T differed markedly from that of the genus Deinococcus. The predominant lipid of strain K4.1T was an unknown aminophospholipid and it did not contain the unknown phosphoglycolipid predominant in the polar lipid profiles of deinococci analysed to date. Two of the predominant fatty acids of the strain, 15 : 0 anteiso and 17 : 0 anteiso, were lacking or present in small amounts in species of the genus Deinococcus. Phylogenetic distinctness and significant differences in the polar lipid and fatty acid profiles suggest classification of strain K4.1T as a novel genus and species in the family Deinococcaceae, for which we propose the name Deinobacterium chartae gen. nov., sp. nov. The type strain is K4.1T (=DSM 21458T =HAMBI 2721T).
A novel, moderately thermophilic, chemo-organotrophic bacterium was isolated from formation fluid samples from an offshore oil-production well head at Bombay High (Western India). Cells were rod-shaped with a sheath-like outer structure (‘toga’); the cells appeared singly, in pairs or in short chains. Cells grew at 25–70 °C (optimum 55–58 °C), pH 5.5–9.0 (optimum pH 7.3–7.8) and 0–12 % (w/v) NaCl (optimum 4.0–4.5 %). The isolate was able to grow on various carbohydrates or complex proteinaceous substances. The isolate reduced thiosulfate and elemental sulfur. The major end products of glucose fermentation were acetate, H2 and CO2. The DNA G+C content of the genomic DNA was 26.8 mol%. Phylogenetic analysis of the 16S rRNA gene placed the strain within the order Thermotogales in the bacterial domain. On the basis of 16S rRNA gene sequence comparisons and in combination with morphological and physiological characteristics, the isolate represents a novel species of new genus, for which the name Oceanotoga teriensis gen. nov., sp. nov. is proposed. The type strain of the type species is OCT74T (=JCM 15580T=LMG 24865T).