A Gram-negative bacterium, designated P2T, was isolated from the biofilm developed on the inner surface of an ultrapure cooling water system in a Hungarian power plant and was characterized by a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain P2T was affiliated with the family ‘Flexibacteraceae’ in the phylum Bacteroidetes. Its closest relative was Flectobacillus lacus CL-GP79T (88.7 % 16S rRNA gene sequence similarity) followed by Arcicella rosea TW5T (86.5 %), Arcicella aquatica NO-502T (86.4 %), Flectobacillus roseus GFA-11T (86.3 %) and Flectobacillus major DSM 103T (85.4 %). Cells of strain P2T were facultatively anaerobic, non-motile rods. The major fatty acids were C16 : 1 ω5c (42.5 %), iso-C15 : 0 2-OH (17.2 %), iso-C17 : 0 3-OH (16.1 %) and iso-C15 : 0 (8.5 %). The major menaquinone was MK-7 and the predominant polar lipid was phosphatidylethanolamine. The DNA G+C content was 54.5 mol%. Thus, the phenotypic and genotypic analyses clearly showed that strain P2T is considerably different from members of other genera in the family ‘Flexibacteraceae’. Based on these results, it is concluded that strain P2T represents a novel species in a new genus, for which the name Siphonobacter aquaeclarae gen. nov., sp. nov. is proposed, with type strain P2T (=DSM 21668T =NCAIM B 02328T).
Two anaerobic, Gram-negative, non-motile and non-spore-forming bacterial strains, designated MAJ27T and MAJ26, were isolated from human faeces. Both isolates grew optimally at 37 °C, were oxidase- and catalase-negative, were sensitive to bile and produced acid from fermentation of several substrates, including glucose. A study based on 16S rRNA gene sequences showed that both isolates were closely related to type strains of species of the genus Bacteroides. Comparisons of the isolates with Bacteroides thetaiotaomicron VPI 5482T and Bacteroides finegoldii JCM 13345T showed high levels of 16S rRNA gene sequence similarity (98.6–98.7 and 96.9–97.0 %, respectively), but low levels of DNA–DNA relatedness (≤22 %). The DNA G+C content (42.7±1 mol%) and the major fatty acid (anteiso-C15 : 0, 39.3–42.5 %) supported the assignment of the isolates to the genus Bacteroides. Based on phenotypic, chemotaxonomic, genotypic and phylogenetic studies, we propose that strains MAJ27T and MAJ26 be classified as representing a novel species, Bacteroides faecis sp. nov. The type strain is MAJ27T (=KCTC 5823T=JCM 16478T).
A Gram-negative, non-motile, aerobic bacterial strain, designated MJ20T, was isolated from farm soil near Daejeon (South Korea) and was characterized taxonomically by using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that strain MJ20T belongs to the family Cytophagaceae, class Sphingobacteria, and was related most closely to Dyadobacter fermentans DSM 18053T (98.9 % sequence similarity), Dyadobacter beijingensis JCM 14200T (98.0 %) and Dyadobacter ginsengisoli KCTC 12589T (96.4 %). The G+C content of the genomic DNA of strain MJ20T was 48.5 mol%. The detection of MK-7 as the predominant menaquinone and a fatty acid profile with summed feature 4 (C16 : 1 ω7c and/or iso-C15 : 0 2-OH), iso-C15 : 0, C16 : 0 and C16 : 1 ω5c as major components supported the affiliation of strain MJ20T to the genus Dyadobacter. The new isolate exhibited relatively low levels of DNA–DNA relatedness with respect to D. fermentans DSM 18053T (mean±sd of three determinations, 47±7 %) and D. beijingensis JCM 14200T (38±8 %). On the basis of its phenotypic and genotypic properties together with phylogenetic distinctiveness, strain MJ20T (=KCTC 22481T =JCM 16232T) should be classified in the genus Dyadobacter as the type strain of a novel species, for which the name Dyadobacter soli sp. nov. is proposed.
A Gram-negative, aerobic, rod-shaped, non-motile bacterium, designated BZ42T, was isolated from the soil of an industrial site. Strain BZ42T was able to grow at 5–25 °C. The major fatty acids were iso-C15 : 0 (46.2 %), C16 : 1 ω7c and/or iso-C15 : 0 2-OH (23.2 %) and iso-C17 : 0 3-OH (9.1 %). The predominant menaquinone was MK-7. The genomic DNA G+C content was 36.5 mol% (HPLC). 16S rRNA gene sequence phylogenetic analysis revealed that strain BZ42T was a member of the genus Pedobacter, family Sphingobacteriaceae, and 16S rRNA gene sequence similarities between strain BZ42T and the type strains of species of the genus Pedobacter with validly published names were 90.4–93.2 %. On the basis of phenotypic, chemotaxonomic and phylogenetic distinctiveness, strain BZ42T was considered to represent a novel species of the genus Pedobacter, for which the name Pedobacter bauzanensis sp. nov. is proposed. The type strain is BZ42T (=DSM 22554T =CGMCC 1.10187T =CIP 110134T).