Anamorphic basidiomycetous yeast strains RS090T and RS092 were isolated from a soil sample collected on Rishiri Island in the Rishiri, Rebun, Sarobetsu National Park, Hokkaido, Japan. As the sequences of the D1/D2 domains of their large-subunit rRNA genes were identical and those of the internal transcribed spacer regions differed in only four bases, we conclude that they belong to a single species with intraspecific diversity. Phylogenetically, this species was related to Dioszegia buhagiarii and Dioszegia hungarica, in the Tremellales, Tremellomycetes, Basidiomycota, but was clearly distinct from them. Based on the results of sequence analyses and phenotypic characteristics, we conclude that they belong to a novel species in the genus Dioszegia, for which the name Dioszegia rishiriensis sp. nov. is proposed, with the type strain RS090T ( = JCM 16282T = CBS 11844T).
Two marine stichotrich ciliates, Metaurostylopsis flavicana spec. nov. and Tunicothrix wilberti (Lin & Song, 2004) Xu et al., 2006, isolated from the Shenzhen Mangrove Protection Area on the coast of the South China Sea, were investigated using live observation and protargol impregnation techniques. Metaurostylopsis flavicana is characterized by its elongate body shape, yellowish body colour and bright-yellow cortical granules that are either grouped around the cirri and the dorsal cilia or aligned between the rows of cirri and dorsal cilia. It has four to eight frontal, two frontoterminal, one buccal and seven to ten transverse cirri, a mid-ventral complex comprising 13–17 midventral cirral pairs in a row that extends about three-fifths of the body length, four left and three right marginal rows and three complete dorsal kineties. The small subunit rRNA gene of this species was sequenced and phylogenetic trees were constructed in which M. flavicana does not group with its congeners, suggesting that the genus Metaurostylopsis is paraphyletic. Some supplementary morphological and morphogenetic traits for Tunicothrix wilberti are also documented.
During a survey of yeasts associated with wood-ingesting insects, six strains of the Sugiyamaella clade were isolated from the gut of passalid and tenebrionid beetles and the decayed wood inhabited by them. Phylogeny based on rRNA gene sequences placed these yeasts as members of Sugiyamaella smithiae, Sugiyamaella americana, Candida lignohabitans and a novel species closely related to Su. americana. The only strain of the novel species, EH008T, could be unquestionably distinguished from its relatives by DNA sequences and other taxonomic characteristics. Ascospore production was not observed under the laboratory conditions tested. Therefore, this novel species is proposed as Candida bullrunensis sp. nov. (type strain EH008T = ATCC MYA-4660T = CBS 11840T).