Two aerobic bacterial strains, designated SP1PR4T and SP1PR5, were isolated from tundra soil samples collected from Saana fjeld, North-western Finland (69° 03′ N 20° 50′ E). Cells of both strains were Gram-negative, non-motile rods. Phylogenetic analysis indicated that the strains belong to the genus Terriglobus in subdivision 1 of the phylum Acidobacteria. Strains SP1PR4T and SP1PR5 shared identical BOX and ERIC fingerprints and 99.7 % 16S rRNA gene similarity indicating that, together with their identical physiological features, these strains are members of the same species. The 16S rRNA gene sequence similarity of SP1PR4T and SP1PR5 with Terriglobus roseus DSM 18391T was 97.1 %. A low DNA–DNA hybridization value (<20 %) and rpoB gene sequence similarity (83.6 %) with T. roseus DSM 18391T indicated that the tundra soil isolates represent novel members of the genus Terriglobus. Strains SP1PR4T and SP1PR5 grew at pH 4.5–7.5 and 4–30 °C. Sugars were the preferred growth substrates. The major cellular fatty acids were iso-C15 : 0, C16 : 1ω7c, iso-C13 : 0 and C16 : 0. The DNA G+C content of strain SP1PR4T was 57.3 mol%. Based on phylogenetic, chemotaxonomic and physiological analyses, the name Terriglobus saanensis sp. nov. is proposed to accommodate the two strains; the type strain is SP1PR4T ( = DSM 23119T = ATCC BAA-1853T).
A Gram-negative, non-spore-forming, endophytic bacterium, strain YC6886T, was isolated from the root of a halophyte, Rosa rugosa, which inhabits coastal areas of Namhae Island off the southern coast of Korea. Cells were non-motile, obligately aerobic rods and formed pale-yellow colonies. The isolate grew at 4–32 °C (optimum 25–28 °C) and at pH 6.5–9.5 (optimum pH 7.5) and grew optimally with 2–3 % (w/v) NaCl, but NaCl was not an absolute requirement for growth. Strain YC6886T produced yellow carotenoid pigments. Strain YC6886T exhibited the highest 16S rRNA gene sequence similarity with Haloferula sargassicola MN1-1037T (97.4 %). Sequence similarities between strain YC6886T and other members of the genus Haloferula were 93.9–94.7 %. DNA–DNA relatedness between strain YC6886T and H. sargassicola KCTC 22202T and Haloferula rosea KCTC 22201T was 27 and 15 %, respectively. The major fatty acids were iso-C14 : 0, C16 : 0 and C16 : 1ω9c and minor components were C14 : 0, C18 : 0 and anteiso-C15 : 0. The major respiratory quinone was menaquinone 9 and the DNA G+C content was 58.5 mol%. The polar lipid profile was composed of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, an unknown phospholipid and an unknown phosphoglycolipid. On the basis of phenotypic, chemotaxonomic, DNA–DNA hybridization and phylogenetic analysis, strain YC6886T represents a novel species in the genus Haloferula, for which the name Haloferula luteola sp. nov. is proposed. The type strain is YC6886T ( = KCTC 22447T = DSM 21608T). An emended description of the genus Haloferula is also presented.