A non-motile and yellow-pigmented bacterium, designated strain HMD3054T, was isolated from a solar saltern in Jeungdo, Republic of Korea. The major fatty acids of strain HMD3054T were iso-C15 : 0 (31.4 %), anteiso-C15 : 0 (23.5 %), iso-C17 : 0 3-OH (14.2 %), summed feature 3 (comprising C16 : 1ω6c and/or C16 : 1ω7c; 6.9 %) and summed feature 9 (comprising iso-C17 : 1ω9c and/or 10-methyl C16 : 0; 6.0 %). The major respiratory quinones were MK-6 and MK-7. The DNA G+C content of strain HMD3054T was 46.9 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain HMD3054T formed a lineage within the genus Echinicola. Strain HMD3054T was closely related to Echinicola vietnamensis KMM 6221T (94.3 % 16S rRNA gene sequence similarity) and Echinicola pacifica KMM 6172T (94.0 %). On the basis of the evidence presented in this study, strain HMD3054T represents a novel species of the genus Echinicola, for which the name Echinicola jeungdonensis sp. nov. is proposed. The type strain is HMD3054T ( = KCTC 23122T = CECT 7682T).
A coccoid- and amorphous-shaped, non-gliding, reddish bacterium, designated SG-29T, was isolated from seawater in the western North Pacific Ocean near Japan. The strain was Gram-stain-negative, obligately aerobic, heterotrophic and catalase-positive. Nitrate was reduced to nitrogen and acid was produced from aesculin, turanose, 2-keto-gluconate and arabinose. Growth occurred with 1–5 % NaCl (optimum 2 % NaCl) and at 5–37 °C (optimum 20–30 °C). The G+C content of genomic DNA was 68.9 mol% (HPLC). The predominant isoprenoid quinone was MK-7 and the major cellular fatty acids (>10 %) were iso-C17 : 1ω9c, C17 : 1ω8c and iso-C17 : 0. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SG-29T belonged to the phylum Bacteroidetes. The most closely related strain was Rhodothermus obamensis JCM 9785T, with which the isolate exhibited 86.1 % 16S rRNA gene sequence similarity. On the basis of phenotypic and phylogenetic features, strain SG-29T is classified in a novel genus and species within the family ‘Rhodothermaceae’, for which the name Rubricoccus marinus gen. nov., sp. nov. ( = NBRC 107124T = KCTC 23197T) is proposed.
As part of an undergraduate microbiology course, a yellow–orange pigmented, Gram-staining negative, rod-shaped, non-motile bacterial strain, designated CTMT, was isolated from a creek in North-central Pennsylvania during the winter of 2006. The 16S rRNA gene sequence of the strain showed ~97 % similarity to that of Chryseobacterium soldanellicola PSD1-4T and Chryseobacterium soli JS6-6T, while the protein-coding gyrB gene sequence of strain CTMT showed <87 % similarity to those of its two closest relatives. Using a polyphasic approach, strain CTMT was characterized and compared to these and other closely related species of the genus Chryseobacterium. Strain CTMT was similar to other strains of the genus Chryseobacterium in that it contained MK-6 as its major respiratory quinone, produced flexirubin-type pigments, oxidase and catalase, hydrolysed DNA, gelatin and aesculin and contained the fatty acids iso-C15 : 0, iso-C17 : 1ω9c, iso-C17 : 0 3-OH and summed feature 3 (C16 : 1ω6c, C16 : 1ω7c and/or iso-C15 : 0 2-OH). Based on the results of this study, strain CTMT represents a novel species of the genus Chryseobacterium, for which the name Chryseobacterium piperi sp. nov. is proposed. The type strain is CTMT ( = ATCC BAA-1782T = CCUG 57707T = JCM 15960T = DSM 22249T = KCTC 23267T).