The taxonomic positions of two actinomycetes isolated from a hay meadow soil sample were determined using a polyphasic approach. The isolates had chemical and morphological properties typical of streptomycetes and formed a distinct 16S rRNA gene subclade together with the type strain Streptomyces drozdowiczii NRRL B-24297T. DNA–DNA relatedness studies showed that the three strains belonged to different genomic species. The organisms were also distinguished using a combination of phenotypic properties. On the basis of these data it is proposed that the isolates be assigned to the genus Streptomyces as Streptomyces brevispora sp. nov. and Streptomyces laculatispora sp. nov., with BK160T ( = KACC 21093T = NCIMB 14702T) and BK166T ( = KACC 20907T = NCIMB 14703T) as the respective type strains.
A Gram-positive, coccoid, non-endospore-forming actinobacterium (strain CCUG 35676T) was isolated from cerebrospinal fluid from a 24-year-old woman in Gothenborg, Sweden. Based on pairwise 16S rRNA gene sequence similarity studies, strain CCUG 35676T was shown to belong to the genus Dietzia and was most closely related to the type strains of Dietzia aerolata (99.3 %), Dietzia lutea (98.8 %), Dietzia schimae (98.5 %), Dietzia maris (98.5 %), Dietzia alimentaria (98.3 %) and Dietzia cercidiphylli (98.0 %). The major menaquinone was MK-8(H2). Major polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol, an unidentified aminophospholipid (APL1), an unidentified phospholipid (PL1) and unidentified glycolipids (GL1 and GL3). Numerous other lipids were also detected. The fatty acid profile, comprising C16 : 0, C17 : 0, C18 : 1ω9c and 10-methyl-C18 : 0 as major fatty acids, supported the affiliation of strain CCUG 35676T to the genus Dietzia. On the basis of the results of physiological and biochemical tests and DNA–DNA hybridizations, a clear phenotypic and genotypic differentiation of strain CCUG 35676T from the most closely related Dietzia species is possible. Strain CCUG 35676T represents a novel species, for which the name Dietzia aurantiaca sp. nov. is proposed, with CCUG 35676T ( = JCM 17645T) as the type strain.
A Gram-positive actinobacterium, strain IV-75T, was isolated by using R2A agar from the ultrapure water system of a power plant in Hungary. The strain exhibited a rod–coccus cell cycle, and was strictly aerobic, non-motile, catalase-positive and oxidase-negative. 16S rRNA gene sequence analysis revealed that strain IV-75T belonged to the suborder Micrococcineae and clustered with members of the family Intrasporangiaceae. Its closest phylogenetic neighbour was Arsenicicoccus bolidensis CCUG 47306T (94.3 % 16S rRNA gene sequence similarity). The peptidoglycan of strain IV-75T contained meso-diaminopimelic acid and MK-10(H4) was the major menaquinone. The polar lipid pattern contained phosphatidylglycerol, two unidentified phospholipids, one glycolipid and several other lipid components. The major fatty acids were anteiso-C15 : 0, C18 : 1ω9c and C16 : 0. Based on the moderate levels of 16S rRNA gene sequence similarity to all members of the family Intrasporangiaceae and the unique combination of chemotaxonomic characteristics, strain IV-75T is considered to represent a novel species of a new genus, for which the name Aquipuribacter hungaricus gen. nov., sp. nov. is proposed. The type strain of Aquipuribacter hungaricus is IV-75T ( = DSM 21674T = NCAIM B 02333T).
A novel actinobacterial strain, designated P4-5T, was isolated from soil of a ginseng field located in Geumsan County, Republic of Korea. Cells of strain P4-5T were Gram-stain-positive, oxidase- and catalase-positive, motile, short rods and the strain produced creamy white colonies on trypticase soy agar. The isolate contained demethylmenaquinone 7 (DMK-7) as the predominant isoprenoid quinone, C18 : 1ω9c and anteiso-C15 : 0 as major fatty acids, diphosphatidylglycerol, phosphatidylglycerol and several unknown lipids in the polar lipid profile, galactose, glucose, mannose, arabinose, xylose (trace) and rhamnose as cell-wall sugars, and meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan. The DNA G+C content of strain P4-5T was 74.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequencing showed that strain P4-5T was related most closely to Patulibacter minatonensis KV-614T and Patulibacter americanus CP177-2T (98.4 and 98.2 % similarity, respectively) and that it formed a separate lineage in the genus Patulibacter. Combined phenotypic and DNA–DNA hybridization data supported the conclusion that strain P4-5T represents a novel species of the genus Patulibacter, for which the name Patulibacter ginsengiterrae sp. nov. is proposed. The type strain is P4-5T ( = KCTC 19427T = CECT 7603T). An emended description of the genus Patulibacter is also provided.
A Gram-reaction-positive, rod-shaped, non-motile, non-spore-forming bacterium (strain BX5-10T) was isolated from the soil of a ginseng field on Baekdu Mountain in Jilin district, China. The taxonomic position of this bacterium was determined in an investigation based on a polyphasic approach. On the basis of 16S rRNA gene sequence analysis, strain BX5-10T was shown to belong to the family Nocardioidaceae and to be most closely related to Nocardioides plantarum NCIMB 12834T (96.5 % sequence similarity), Nocardioides dokdonensis KCTC 19309T (96.2 %) and Nocardioides fonticola NAA-13T (95.1 %). Strain BX5-10T was characterized chemotaxonomically as having ll-2,6-diaminopimelic acid in its cell-wall peptidoglycan, MK-8(H4) as the predominant menaquinone and C18 : 1ω9c, C16 : 0 and C17 : 1ω8c as its major fatty acids. The G+C content of the genomic DNA was 70.3 mol%. The novel strain could be differentiated genotypically and phenotypically from all recognized species of the genus Nocardioides. Based on the results of the phylogenetic analyses and the genotypic and phenotypic data, a novel species, Nocardioides ginsengagri sp. nov., is proposed. The type strain is BX5-10T ( = KCTC 19467T = DSM 21362T).
A Streptomyces-like strain, 172205T, was obtained from mangrove soil collected at Qinglan Harbour, Wenchang, Hainan, China. The strain was characterized by white aerial mycelium and long spore chains. Comparison of 16S rRNA gene sequences indicated that the strain represents a novel member of the genus Streptomyces, exhibiting highest levels of similarity (<98.29 %) to the type strains of members of the genus Streptomyces. However, DNA–DNA relatedness and phenotypic data readily distinguished strain 172205T from phylogenetically related type strains. The predominant menaquinones were MK-9(H6) and MK-9(H8). The major fatty acids were iso-C15 : 0 (10.31 %), anteiso-C15 : 0 (35.19 %), iso-C16 : 0 (20.24 %) and anteiso-C17 : 0 (10.05 %). The diagnostic phospholipid was phosphatidylethanolamine. The cell wall contained ll-diaminopimelic acid and meso-diaminopimelic acid and whole-cell hydrolysates contained ribose, galactose and glucose. The results of DNA–DNA hybridization, physiological and biochemical tests allowed the genotypic and phenotypic differentiation of strain 172205T from phylogenetically related type strains. Therefore, strain 172205T is considered to represent a novel species of the genus Streptomyces, for which the name Streptomyces qinglanensis sp. nov. is proposed. The type strain is 172205T ( = CGMCC 4.6825T = DSM 42035T).
A Gram-stain-positive, non-spore-forming bacterium (GW5-5797T) was isolated on soil extract agar from sand collected at a depth of 5 m in the Caribbean Sea near Grenada. 16S rRNA gene sequence analysis and similarity studies showed that strain GW5-5797T belongs to the genus Nocardia, and is most closely related to Nocardia speluncae N2-11T (99.2 % similarity) and Nocardia jinanensis 04-5195T (99.2 %) and more distantly related to Nocardia rhamnosiphila 202GMOT (98.6 %) and other Nocardia species. Strain GW5-5797T could be distinguished from all other recognized Nocardia species by sequence similarity values less than 98.5 %. The peptidoglycan diamino acid was meso-diaminopimelic acid. Strain GW5-5797T exhibited a quinone system with the predominant compounds MK-8(H4ω-cyclo) and MK-8(H2). The polar lipid profile of GW5-5797T consisted of the major compounds diphosphatidylglycerol, phosphatidylethanolamine and an unidentified glycolipid, moderate amounts of phosphatidylinositol and a phosphatidylinositol mannoside and minor amounts of several lipids including a second phosphatidylinositol mannoside. The polyamine pattern contained the major compound spermine and moderate amounts of spermidine. The major fatty acids were C16 : 0, C18 : 1ω9c and 10-methyl C18 : 0. These chemotaxonomic traits are in excellent agreement with those of other Nocardia species. The results of DNA–DNA hybridizations and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain GW5-5797T from the most closely related species, showing 16S rRNA gene sequence similarities >98.5 %. Strain GW5-5797T therefore merits separate species status, and we propose the name Nocardia grenadensis sp. nov., with the type strain GW5-5797T ( = CCUG 60970T = CIP 110294T).