During an investigation of yeast biota in the rhizosphere of lentisk in Sardinian semi-arid areas, a strain was isolated that could not be assigned to any known species. The sequence of the D1/D2 domain of the large subunit rDNA gene revealed that the strain belonged to the genus Kazachstania and was phylogenetically related to a clade including Kazachstania aerobia, Kazachstania servazzii, Kazachstania solicola and Kazachstania unispora. The novel isolate differed from members of this clade in its ability to assimilate d-glucono-1,5-lactone and its very weak fermentation of glucose and sucrose; its assimilation profile was unique within the genus Kazachstania. Monosporal colonies were able to sporulate, indicating that the species is homothallic. It is proposed that the isolate represents a novel species, Kazachstania ichnusensis sp. nov., with LCF 1675T ( = CBS 11859T) as type strain.
Two strains of a novel teleomorphic basidiomycete were isolated from grassland soil. Standard phenotypic tests and phylogenetic analyses of 26S rRNA gene (D1/D2 domains) and ITS region sequences showed that the species belongs to the core group of the genus Leucosporidium. A novel species, Leucosporidium drummii sp. nov., is proposed to accommodate the two strains, with SEG-3-2-AY220T ( = CBS 11562T = MUCL 52878T) as the type strain. In addition, phylogenetic analysis revealed great genetic variability in the Leucosporidium scottii complex.
The morphology of three marine colepid ciliates, Nolandia sinica spec. nov., Apocoleps caoi spec. nov. and Tiarina fusa (Claparède & Lachmann, 1858) Bergh, 1881, isolated from Chinese coastal waters, was investigated. N. sinica spec. nov. may be separated from its congeners by the structure of its armour plates, each of which may have up to five reniform windows. A. caoi spec. nov. is characterized by its large body with broad anterior end and by having 10–12 long, sharp posterior spines. New data and an improved diagnosis are supplied for Tiarina fusa (Claparède & Lachmann, 1858) Bergh, 1881, which has a spindle-shaped body, about 16 ciliary rows and a single adoral organelle. Sequence similarities with other available colepid species were determined.
A novel avian trypanosome, Trypanosoma culicavium sp. nov., isolated from Culex mosquitoes, is described on the basis of naturally and experimentally infected vectors and bird hosts, localization in the vector, morphological characters and molecular data. This study provides the first comprehensive description of a trypanosome species transmitted by mosquitoes, in which parasites form plugs and rosettes on the stomodeal valve. Trypanosomes occurred as long epimastigotes and short trypomastigotes in vectors and culture and as long trypomastigotes in birds. Transmission of parasites to bird hosts was achieved exclusively by ingestion of experimentally infected Culex mosquito females by canaries (Serinus canaria), but not by Japanese quails (Coturnix japonica), nor by the bite of infected vectors, nor by ingestion of parasites from laboratory cultures. Transmission experiments and the identity of isolates from collared flycatchers (Ficedula albicollis) and Culex mosquitoes suggests that the natural hosts of T. culicavium are insectivorous songbirds (Passeriformes). Phylogenetic analyses of small-subunit rRNA and glycosomal glyceraldehyde-3-phosphate dehydrogenase gene sequences demonstrated that T. culicavium sp. nov. is more related to Trypanosoma corvi than to other avian trypanosomes (e.g. Trypanosoma avium and Trypanosoma bennetti).