A Gram-stain-positive, strictly aerobic, spherical, non-motile red-pigmented bacterial strain, designated MJ27T, was isolated from a sludge sample of the Daejeon sewage disposal plant in South Korea. A polyphasic approach was used to study the taxonomic position of strain MJ27T. Strain MJ27T shared highest 16S rRNA gene sequence similarity with Deinococcus grandis DSM 3963T (98.8 %), Deinococcus caeni Ho-08T (97.5 %) and Deinococcus aquaticus PB314T (96.6 %.); levels of sequence similarity with the type strains of other Deinococcus species were less than 96.0 %. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain MJ27T belonged to the clade formed by members of the genus Deinococcus in the family Deinococcaceae . The G+C content of the genomic DNA of strain MJ27T was 67.6 mol%. The chemotaxonomic characteristics of strain MJ27T were typical of members of the genus Deinococcus , with MK-8 as the predominant respiratory quinone, C16 : 1ω7c, C15 : 1ω6c, C16 : 0 and C15 : 0 as major fatty acids (>12 %), ornithine as the diamino acid in the cell-wall peptidoglycan and resistance to gamma radiation [D10 (dose required to reduce the bacterial population by tenfold) >9 kGy]. The low levels of DNA–DNA relatedness reported here (5.3±1.5–29.2±2.3 %) indicate that strain MJ27T represents a species that is separate from its closest relatives in the genus Deinococcus . On the basis of phylogenetic inference, fatty acid profile and other phenotypic properties, strain MJ27T is considered to represent a novel species of the genus Deinococcus , for which the name Deinococcus daejeonensis sp. nov. is proposed. The type strain is MJ27T ( = KCTC 13751T = JCM 16918T).
Six strains with the typical characteristics of mycoplasmas were isolated from the tracheae of six Canarian Egyptian vultures (Neophron percnopterus majorensis). The results of biochemical, serological and molecular genetic studies showed that the isolates were nearly identical and that they could be considered as representing a novel species of the genus Mycoplasma . Colonies possessed the typical fried-egg appearance and electron micrographs revealed a pleomorphic cellular morphology with the lack of a cell wall. The isolates hydrolysed arginine and required sterol for growth but did not ferment glucose or hydrolyse urea. We propose that the isolates be assigned to a novel species,Mycoplasma neophronis sp. nov. The type strain is G.A.T ( = DSM 24097T = ATCC BAA-2157T). The antiserum of strain G.A.T has been deposited in the Mollicutes collection at Purdue University (Indiana, USA).
Strain SulfLac1T, a thermophilic, anaerobic and slightly halophilic, rod-shaped bacterium with a sheath-like outer structure (toga), was isolated from a whey digester in Tunisia. The strain’s non-motile cells measured 3–30×1 µm and appeared singly, in pairs or as long chains. The novel strain reduced thiosulfate and elemental sulfur, but not sulfate or sulfite, into sulfide. It grew at 37–65 °C (optimum 55 °C), at pH 6.5–7.9 (optimum pH 6.9) and with 0.2–3 % (w/v) NaCl (optimum 0.5 %). The G+C content of the strain’s genomic DNA was 33.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SulfLac1T was most closely related to Petrotoga mobilis (91.4 % sequence similarity). Based on phenotypic, phylogenetic and chemotaxonomic evidence, strain SulfLac1T represents a novel species of a new genus within the order Thermotogales , for which the name Defluviitoga tunisiensis gen. nov., sp. nov. is proposed. The type strain of the type species is SulfLac1T ( = DSM 23805T = JCM 17210T).