A Gram-stain-negative, non-spore-forming, non-motile, strictly aerobic, rod-shaped bacterial strain, designated CAU 1074T, was isolated from marine sediment and its taxonomic position was investigated using a polyphasic approach. Strain CAU 1074T grew optimally at 30 °C and pH 6.5. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CAU 1074T formed a distinct lineage within the genus Haloferula and was most closely related to Haloferula harenae KCTC 22198T (96.0 % similarity). Strain CAU 1074T contained MK-9 as the major isoprenoid quinone, and iso-C14 : 0, C16 : 1ω9c and C16 : 0 as the major fatty acids. The cell wall peptidoglycan contained meso-diaminopimelic acid. The major whole-cell sugars were glucose, xylose, mannose and ribose. The polar lipids were composed of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, aminoglycolipid and two unidentified phospholipids. The DNA G+C content of the strain was 64.0 mol%. On the basis of phenotypic and chemotaxonomic data, and phylogenetic inference, strain CAU 1074T should be classified as a member of a novel species in the genus Haloferula , for which the name Haloferula chungangensis sp. nov. is proposed; the type strain is CAU 1074T ( = KCTC 23578T = CCUG 61920T). An emended description of the genus Haloferula is also provided.
A novel obligately anaerobic, mesophilic, organotrophic bacterium, strain P3M-1T, was isolated from a microbial mat formed in a wooden bath filled with hot water emerging from a 2775 m-deep well in the Tomsk region of western Siberia, Russia. Cells of strain P3M-1T were rod-shaped, 0.3–0.7 µm in width and formed multicellullar filaments that reached up to 400 µm in length. Strain P3M-1T grew optimally at 42–45 °C, pH 7.5–8.0, and with 0.1% (w/v) NaCl. Under optimal conditions, the doubling time was 6 h. The isolate was able to ferment a variety of proteinaceous substrates and sugars, including microcrystalline cellulose. Acetate, ethanol and H2 were the main products of glucose fermentation. The genomic DNA G+C content was 55 mol%. 16S rRNA gene sequence-based phylogenetic analyses showed that strain P3M-1T was a member of the class Anaerolinea , with 92.8 % sequence similarity to Levilinea saccharolytica KIBI-1T. Based on phylogenetic analysis and physiological properties, strain P3M-1T represents a novel species in a new genus, for which the name Ornatilinea apprima gen. nov., sp. nov. is proposed; the type strain of O. apprima is P3M-1T ( = DSM 23815T = VKM B-2669T).