A Gram-staining-negative, aerobic, non-flagellated, non-gliding and ring-like or horseshoe-shaped bacterial strain, designated HD-17T, was isolated from a tidal flat sediment in the Korean peninsula. Strain HD-17T grew optimally at pH 7.0–8.0, at 25 °C and in the presence of 2.0 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain HD-17T fell within the clade comprising species of the genus Cyclobacterium . Strain HD-17T exhibited 16S rRNA gene sequence similarity values of 93.8–98.8 % to the type strains of species of the genus Cyclobacterium . Strain HD-17T contained MK-7 as the predominant menaquinone and iso-C15 : 0, summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c), anteiso-C15 : 0 and iso-C15 : 1 G as the major fatty acids. The major polar lipids of strain HD-17T were phosphatidylcholine, phosphatidylethanolamine and two unidentified lipids. The DNA G+C content of strain HD-17T was 43.8 mol% and its DNA–DNA relatedness values with Cyclobacterium amurskyense KCTC 12363T, Cyclobacterium qasimii KCTC 23011T and Cyclobacterium marinum KCTC 2917T were 10.4, 7.6 and 5.3 %, respectively. The phylogenetic and genetic distinctiveness and several differentiating phenotypic properties revealed that strain HD-17T was separate from other species of the genus Cyclobacterium . On the basis of the data presented, strain HD-17T represents a novel species of the genus Cyclobacterium , for which the name Cyclobacterium caenipelagi sp. nov. is proposed. The type strain is HD-17T ( = KCTC 32178T = CCUG 63247T). An emended description of the genus Cyclobacterium is also provided.
A novel bacterial strain, designated LCJ02T, was isolated on R2A agar from donkey (Equus asinus) milk powder and subjected to a taxonomic study using a polyphasic approach. Strain LCJ02T showed a Gram-negative reaction, was non-motile, non-spore-forming and possessed rod-shaped cells and yellow-pigmented colonies. Phylogenetic analysis based on 16S rRNA gene sequences showed that the novel isolate formed a cluster with several uncultured bacterial clones and with cultured members of the genera Hydrotalea , Sediminibacterium and Lacibacter (family Chitinophagaceae , phylum Bacteroidetes ). The gene sequence similarities with respect to the type strains of recognized species from the above genera and other phylogenetic neighbours ranged from 89.3 to 92.9 %. The G+C content of the genomic DNA was 49.2 mol%, the only isoprenoid quinone was MK-7 and the major fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH, iso-C15 : 1 G and summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH). The major polar lipids of strain LCJ02T were phosphatidylethanolamine, two unidentified aminophospholipids, one unidentified aminolipid and five unidentified lipids. The results of physiological and biochemical tests allowed phenotypic differentiation of strain LCJ02T from its closest phylogenetic neighbours. On the basis of the evidence of this polyphasic study, isolate LCJ02T represents a novel genus and species in the family Chitinophagaceae for which the name Asinibacterium lactis gen. nov., sp. nov. is proposed. The type strain is LCJ02T ( = KCCM 90108T = JCM 18484T).