A novel bacterial strain ZT was isolated from the high-arsenic sediment in Jianghan Plain, China. The strain was Gram-staining-negative, rod-shaped and formed yellow colonies. This bacterium is capable of tolerating arsenate and arsenite, with MICs of 40 mM and 20 mM, respectively. The strain also possesses catalase and does not produce oxidase. The nucleotide sequence of the 16S rRNA gene of the isolate showed the highest similarity (96.9 %) to that of the type strain of Flavobacterium soli . On the basis of the 16S rRNA gene sequence analysis and the phenotypic properties of strain ZT, it was assigned to the genus Flavobacterium . The major respiratory menaquinone was MK-6 and the predominant fatty acids were iso-C15 : 0, summed feature 3 (containing C16 : 1ω6c and/or C16 : 1ω7c) and iso-C15 : 1G. The major polar lipids were phosphatidylethanolamine, three uncharacterized aminophospholipids and four unidentified phospholipids. The DNA G+C content was 32.1 mol%. Based on the phenotypic and genotypic data presented in this article, it can be concluded that this isolate represents a novel species of the genus Flavobacterium , for which the name Flavobacterium arsenatis sp. nov. is proposed. The type strain is ZT ( = CCTCC AB 2013048T = KCTC 32397T).
A novel Gram-stain-negative bacteria, designated S37T, was isolated from soil of the Xixi wetland, Zhejiang province, China. Cells of strain S37T were aerobic, non-motile rods. Growth occurred at 10–37 °C (optimum, 25 °C), pH 5.0–9.7 (optimum, pH 7.5) and with 0–6 % (w/v) NaCl (optimum, 0.5 %). Based on 16S rRNA gene sequence analysis, strain S37T was found to be a member of the genus Sphingobacterium and shared highest similarity with Sphingobacterium composti 4M24T (95.78 %). The major fatty acids were summed feature 3 (iso-C15 : 0 2-OH and/or C16 : 1ω7c), iso-C15 : 0 and iso-C17 : 0 3-OH, and the DNA G+C content was 43.8 mol%. The predominant respiratory quinone was MK-7. Based on its phenotypic and chemotaxonomic characteristics and phylogenetic data, strain S37T represents a novel species of the genus Sphingobacterium , for which the name Sphingobacterium paludis sp. nov. (type strain S37T = CGMCC 1.12801T = NBRC 110386T) is proposed.
A taxonomic study was carried out on a novel bacterial strain, designated MIC1008T, which was isolated from the Gomso salt pan, located in Buan County, Korea. Cells were Gram-stain-negative, aerobic, non-motile and moderately halophilic rods, 0.2–0.4 µm wide and 0.7–1.2 µm long. The optimum temperature and pH for growth were 25 °C and pH 6.5–8.5, respectively. Catalase and oxidase activities were positive. Carotenoid pigments were produced. Comparison of 16S rRNA gene sequences indicated that the isolate belonged to the genus Psychroflexus , with Psychroflexus halocasei WCC 4520T as its closest relative, with a 16S rRNA gene sequence similarity of 95.4 %. The isolate contained iso-C15 : 0, iso-C15 : 1 G, iso-C17 : 0 3-OH, anteiso-C15 : 0 and iso-C15 : 0 3-OH as the major fatty acids and menaquinone MK-6 as the isoprenoid quinone. The major polar lipid profile of strain MIC1008T revealed the presence of phosphatidylethanolamine, an unidentified aminolipid and two unidentified lipids. The DNA G+C content of the isolate was 32.9 mol%. On the basis of the evidences presented, it is concluded that strain MIC1008T represents a novel species of the genus Psychroflexus within the family Flavobacteriaceae , for which the name Psychroflexus http://dx.doi.org/10.1601/nm.8182 salarius sp. nov. is proposed. The type strain is MIC1008T ( = KACC 17063T = DSM 25661T).
A novel Gram-stain-negative, facultatively anaerobic, catalase- and oxidase-positive, non-motile and pink-pigmented bacterium, designated G22T, was isolated from Gahai, a saltwater lake in Qinghai province, China. Optimal growth occurred at 33–35 °C, pH 7.0–7.5, and in the presence of 2–4 % (w/v) NaCl. The DNA G+C content was 40.0 mol%. The major polar lipids were phosphatidylethanolamine and three unknown lipids. The predominant cellular fatty acids were iso-C15 : 0, anteiso-C15 : 0, iso-C17 : 0 3-OH and iso-C15 : 0 3-OH, and MK-7 was the main respiratory quinone. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain G22T fell within the class Bacteroidia . Its closest phylogenetic neighbour was the recently described species Draconibacterium orientale , the sole member of the family Draconibacteriaceae , with merely 90.04 % sequence similarity. On the basis of phenotypic, chemotaxonomic and phylogenetic evidence observed, a novel species in a new genus, Tangfeifania diversioriginum gen. nov., sp. nov., is proposed within the family Draconibacteriaceae . The type strain is G22T ( = CICC 10587T = DSM 27063T).
A novel Gram-staining-negative, aerobic, rod-shaped, non-motile, reddish-orange and chemoheterotrophic bacteria, designated strain KD52T, was isolated from a culture of the alga Phaeodactylum tricornutum from Xiamen, Fujian Province, China. 16S rRNA gene sequence comparison showed that strain KD52T was a member of the family Saprospiraceae , forming a distinct lineage with ‘Portibacter lacus’ KCTC 23747. The 16S rRNA gene sequence similarity between strain KD52T and the type strains of species of the family Saprospiraceae ranged from 86 % to 89 %. Growth occurred at 20–37 °C (optimum, 28 °C), in the presence of 1–9 % (w/v) NaCl (optimum, 2.5 %) and at pH 5–8.5 (optimum, pH 6.0). The dominant fatty acids (>10 %) of strain KD52T were iso-C15 : 0 (33.1 %), iso-C15 : 1 G (14.8 %) and summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c, 13.8 %). The major polar lipids were diphosphatidylglycerol, three unidentified phospholipids, four unknown lipids and one unidentified aminolipid. The DNA G+C content was 51 mol% and the major respiratory quinone was menaquinone-7 (MK-7). On the basis of phenotypic data and phylogenetic inference, strain KD52T represents a novel species of a new genus, for which the name Phaeodactylibacter xiamenensis gen. nov., sp. nov., is proposed. The type strain is KD52T ( = MCCC 1F01213T = KCTC 32575T).