A novel yeast species is described from 19 strains isolated from flowers and insects in three provinces of Cuba. The species is so far known only from Cuba. Characteristic asci and ascospores as well as phylogenetic analysis of the rDNA sequence place the novel species in the genus Metschnikowia. The novel species belongs to the New World subclade of large-spored species of Metschnikowia. Mating tests with other members of the subclade resulted in the formation of sterile asci without ascospores, showing that the Cuban strains represent a distinct biological species. Intraspecies matings lead to the production of fertile asci containing large needle-shaped ascospores. The novel species was further distinguished from its close relatives by rDNA sequences and PCR fingerprinting using primers derived from mini- and microsatellites. We propose the name Metschnikowia cubensis sp. nov. and designate MUCL 45753T (=CRGF 279T =CBS 10832T, h+) as the type strain and MUCL 45751 (=CRGF 278 =CBS 10833, h−) as the allotype.
Strain SN-82T was isolated from the sea-surface microlayer at Keelung on the north-east coast of Taiwan. Sequence analysis of the D1/D2 domain of the 26S rDNA of strain SN-82T suggested that this strain is related to the aerius clade in the Filobasidiales. Phenotypic characteristics such as the absence of sexual structures and ballistoconidia, the assimilation of myo-inositol and d-glucuronate, the inability to ferment glucose, the possession of coenzyme Q-10 and positive Diazonium blue B and urease reactions also indicated that this strain belongs to the genus Cryptococcus. However, divergences of more than 3.6 % were observed in the D1/D2 domain when compared with other described Cryptococcus species, which indicated that the isolated yeast represents a previously unrecognized member of this genus. Therefore, the novel yeast species Cryptococcus keelungensis sp. nov. is proposed, with strain SN-82T (=CBS 10876T =BCRC 23107T =JCM 14893T) as the type strain.
A novel psychrophilic basidiomycetous species is described in a new anamorphic genus as Mrakiella cryoconiti gen. nov., sp. nov.; the type strain of Mrakiella cryoconiti is strain A15T (=CBS 10834T =DSM 21094T). Two representatives were isolated from alpine glacier cryoconite and from northern Siberian sediment. Physiological and biochemical properties are similar to characteristics shared by members of the genus Mrakia, although sexual reproduction is absent. Mrakiella cryoconiti strains are psychrophilic and produce cold-active pectate lyase. Sequence analyses of the ITS and 26S rRNA D1/D2 regions indicated that these strains represent a distinct taxon within the Mrakia clade of the order Cystofilobasidiales, class Tremellomycetes and phylum Basidiomycota. On the basis of phenotypic and genotypic characteristics, Cryptococcus aquaticus (a member of the Mrakia clade) is transferred to the newly described genus as Mrakiella aquatica comb. nov.
Three strains (JCM 15063T, JCM 15098 and JCM 15099) of a novel basidiomycetous yeast species belonging to the genus Sporobolomyces were isolated from nasal smears of Queensland koalas kept in a Japanese zoological park. Analyses of sequences of the nuclear rDNA internal transcribed spacer region and the 26S rDNA D1/D2 domain and morphological studies indicated that these strains represent a novel species with a close phylogenetic relationship to Sporobolomyces carnicolor and Sporobolomyces japonicus in the Sporidiobolus lineage, for which the name Sporobolomyces koalae sp. nov. is proposed (type strain JCM 15063T =CBS 10914T =DSM 19992T).