A moderately halophilic, Gram-positive coccus, designated strain W24T, was isolated from saline soil in Qinghai province, China. The isolate was able to grow at salinities of 0–24 % (w/v) NaCl (optimally at 8 %, w/v), at pH 5.5–9.0 (optimally at pH 7.5) and at 8–43 °C (optimally at 28 °C). The genomic DNA G+C content of strain W24T was 45.8 mol%. The predominant isoprenoid quinone was MK-6 and the cell wall contained lysine and glycine as diagnostic diamino acids. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol and an unidentified glycolipid. The major cellular fatty acids were iso-C15 : 0, anteiso-C15 : 0 and C16 : 0. Based on 16S rRNA gene sequence analysis, strain W24T was found to be a member of the genus Salinicoccus and was related most closely to Salinicoccus hispanicus DSM 5352T (96.5 % sequence similarity). Based on data from the current polyphasic study, strain W24T is considered to represent a novel species of the genus Salinicoccus, for which the name Salinicoccus halodurans sp. nov. is proposed. The type strain is W24T (=CGMCC 1.6501T=DSM 19336T).
The taxonomic position of a lactic acid bacterium, strain L13T, isolated from senmaizuke, a Japanese traditional pickle, was studied. This strain was a heterofermentative, facultatively anaerobic, Gram-positive, non-spore-forming, non-motile, rod-shaped bacterium. It produced dl-lactic acid from glucose. Phylogenetic analysis of its 16S rRNA gene sequence and physiological and biochemical characteristics indicated that the strain was a member of the genus Lactobacillus. Based on 16S rRNA gene sequence similarity, strain L13T was closely related to Lactobacillus hammesii CIP 108387T (98.9 %) and Lactobacillus parabrevis ATCC 53295T (98.5 %). In addition, the rpoA gene sequence of strain L13T was closely related to those of L. hammesii CIP 108387T (92.2 %) and L. parabrevis ATCC 53295T (92.0 %). However, DNA–DNA hybridization of strain L13T with these two strains revealed that strain L13T represents a separate genomic species. The DNA G+C content of strain L13T was 46 mol% and the peptidoglycan was of the A4α l-Lys–d-Asp type. Thus, these data indicate that strain L13T represents a novel species of the genus Lactobacillus, for which the name Lactobacillus senmaizukei is proposed. The type strain is L13T (=NBRC 103853T=TISTR 1847T).
A Gram-positive and catalase-negative coccus that formed chains, designated strain FP48-3T, isolated from fermented sausage (‘mum’), was studied systematically. Strain FP48-3T was facultatively anaerobic and produced l-lactic acid from glucose. Straight-chain fatty acids C18 : 1 and C16 : 0 were the dominant components. The DNA G+C content of strain FP48-3T was 37.9 mol%. On the basis of 16S rRNA and RNA polymerase α-subunit (rpoA) gene sequence analysis, strain FP48-3T was closely related to Enterococcus hirae LMG 6399T, Enterococcus durans LMG 10746T and Enterococcus faecium LMG 11423T, with 99.3–99.6 and 95.1–96.9 % sequence similarities, respectively. Strain FP48-3T could be clearly distinguished from E. hirae LMG 6399T, E. durans LMG 10746T and E. faecium LMG 11423T by low DNA–DNA relatedness (≤14 %) and phenotypic characteristics. Therefore, this strain represents a novel species of the genus Enterococcus, for which the name Enterococcus thailandicus sp. nov. is proposed. The type strain is FP48-3T (=KCTC 13134T=NBRC 101867T=NRIC 0107T=TISTR 933T=PCU 282T).