Strains 10-46T and 10-106 were isolated from a water reservoir in Korea. They were Gram-negative, non-motile, non-spore-forming rods, produced yellow-pigmented colonies on nutrient agar, contained MK-6 as the predominant menaquinone and 15 : 0 iso and 17 : 0 iso 3-OH as the major fatty acids and had polar lipid profiles consisting of phosphatidylethanolamine and several unknown aminolipids. Phylogenetic analysis, based on 16S rRNA gene sequencing, showed that the strains were closely related to Chryseobacterium indoltheticum ATCC 27950T, Chryseobacterium scophthalmum LMG 13028T and Chryseobacterium soldanellicola KCTC 12382T (96.9, 96.8 and 96.7 % sequence similarity, respectively) and formed a separate lineage within the genus Chryseobacterium. The combined genotypic and phenotypic data supported the conclusion that the strains represent a novel species of the genus Chryseobacterium, for which the name Chryseobacterium aquaticum sp. nov. is proposed. The type strain is 10-46T (=KCTC 12483T =CECT 7302T).
A rod-shaped, translucent yellow-pigmented, Gram-negative bacterium, strain B390T, was isolated from the bryozoan Schizobrachiella sanguinea collected in the Adriatic Sea, near Rovinj, Croatia. 16S rRNA gene sequence analysis indicated affiliation to the genus Tenacibaculum, with sequence similarity levels of 94.8–97.3 % to type strains of species with validly published names. It grew at 5–34 °C, with optimal growth at 18–26 °C, and only in the presence of NaCl or sea salts. In contrast to other type strains of the genus, strain B390T was able to hydrolyse aesculin. The predominant menaquinone was MK-6 and major fatty acids were iso-C15 : 0, iso-C15 : 0 3-OH and iso-C15 : 1. The DNA G+C content was 31.6 mol%. DNA–DNA hybridization and comparative physiological tests were performed with type strains Tenacibaculum aestuarii JCM 13491T and Tenacibaculum lutimaris DSM 16505T, since they exhibit 16S rRNA gene sequence similarities above 97 %. These data, as well as phylogenetic analyses, suggest that strain B390T (=DSM 18961T =JCM 14633T) should be classified as the type strain of a novel species within the genus Tenacibaculum, for which the name Tenacibaculum adriaticum sp. nov. is proposed.
A yellow-pigmented, Gram-negative, strictly aerobic, rod-shaped bacterium (TDMA-5T) was isolated from a freshwater sample collected at Misasa (Tottori, Japan). The DNA G+C content was 38.6 mol%. Major fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH and summed feature 4 (iso-C15 : 0 2-OH and/or C16 : 1 ω7c). MK-7 was the predominant respiratory quinone. Zeaxanthin was the major carotenoid pigment produced; flexirubin-type pigments were not produced. TDMA-5T was sensitive to gamma-irradiation. The strain degraded gelatin, casein, starch, Tween 80 and DNA. Phylogenetic analysis based on the 16S rRNA gene sequence placed TDMA-5T in a distinct lineage in the family Sphingobacteriaceae, sharing 89.4–93.4 % sequence similarity with members of the nearest genus Pedobacter. Strain TDMA-5T could be distinguished from the other members of the family Sphingobacteriaceae by a number of chemotaxonomic and phenotypic characteristics. Based on its unique phenotypic, genotypic and phylogenetic features, strain TDMA-5T represents a novel genus and species, for which the name Nubsella zeaxanthinifaciens gen. nov., sp. nov. is proposed. The type strain of Nubsella zeaxanthinifaciens is TDMA-5T (=NBRC 102579T =CCUG 54348T).
A non-motile, yellow-pigmented bacterium, designated strain CW9T, was isolated from a water-cooling system in Gwangyang, Republic of Korea. The cells were Gram-negative, catalase- and oxidase-positive, short rods. The major fatty acids were iso-C15 : 0 (45.5 %), iso-C17 : 1 ω9c (14.4 %), iso-C17 : 0 3-OH (13.0 %) and summed feature 3 (comprising iso-C15 : 0 2-OH and/or C16 : 1 ω7c; 8.8 %). The DNA G+C content was 35.6 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain CW9T formed a lineage within the genus Chryseobacterium and was closely related to Chryseobacterium gleum ATCC 35910T (98.4 % sequence similarity) and Chryseobacterium indologenes ATCC 29897T (97.8 % sequence similarity). Phenotypic characteristics and DNA–DNA relatedness data served to distinguish strain CW9T from these two species. On the basis of the evidence presented in this study, strain CW9T represents a novel species of the genus Chryseobacterium, for which the name Chryseobacterium aquifrigidense sp. nov. is proposed. The type strain is CW9T (=KCTC 12894T=JCM 14756T).