A novel aerobic, Gram-negative bacterium, designated strain CM41_14bT, was isolated from surface waters in the coastal north-western Mediterranean Sea. Cells were non-motile, straight rods, 2.6 μm long and 0.7 μm wide and formed pale-orange colonies on marine agar medium. The G+C content of the genomic DNA was 39 mol%. Phylogenetic analysis based on the 16S rRNA gene sequence placed the strain within the genus Balneola (phylum Bacteroidetes). On the basis of 16S rRNA gene sequence comparisons, and physiological and biochemical characteristics, the isolate represents a novel species for which the name Balneola alkaliphila sp. nov. is proposed. The type strain is CM41_14bT (=DSM 19538T=CIP 109603T=OOB 103T).
A Gram-negative, non-motile, aerobic bacterium, designated strain KOPRI 20941T, was isolated from a sample of marine sediment from Ny Ålesund, Spitsbergen, Norway. A phylogenetic analysis based on 16S rRNA gene sequences revealed that the Arctic isolate nested within the genus Maribacter and showed the highest sequence similarity (98.1 %) with respect to Maribacter orientalis KMM 3947T. Chemotaxonomic data (DNA G+C content of 36 mol%; MK-6 as the major respiratory quinone and iso-C17 : 0 3-OH, C16 : 1 ω7c/iso-C15 : 0 2-OH and iso-C15 : 0 as the major fatty acids) supported the affiliation of strain KOPRI 20941T to the genus Maribacter. The results of phylogenetic analyses, physiological and biochemical tests and a DNA–DNA reassociation test (<54 % relatedness) allowed genotypic and phenotypic differentiation of the strain from the recognized species of the genus Maribacter. Therefore strain KOPRI 20941T represents a novel species of the genus Maribacter, for which the name Maribacter arcticus sp. nov. is proposed. The type strain is KOPRI 20941T (=KCTC 22053T=JCM 14790T).
A Gram-negative, non-spore-forming, non-motile, yellow-pigmented, strictly aerobic bacterial strain, designated En5T, was isolated from the East Sea of Korea and was subjected to a polyphasic taxonomy study. Strain En5T grew optimally at 30 °C, in the presence of 1–3 % (w/v) NaCl and at pH 5.3–7.6. The major respiratory lipoquinone was MK-6 and the major fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH and iso-C17 : 1 ω9c. The DNA G+C content of strain En5T was 30.1 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain En5T formed a distinct evolutionary lineage within the family Flavobacteriaceae and shared 93 % sequence similarity with the type strains of both Galbibacter mesophilus and Zhouia amylolytica. On the basis of its phenotypic and phylogenetic properties, strain En5T is suggested to represent a novel species of a new genus in the family Flavobacteriaceae, for which the name Joostella marina gen. nov., sp. nov. is proposed. The type strain is En5T (=KCTC 12518T=DSM 19592T=CGMCC 1.6973T).
The taxonomic position of a novel Gram-negative strain, designated SY1T, isolated from a farm-soil sample obtained from Jiangsu Province, PR China, was characterized by using a polyphasic approach. The cells were non-motile, non-spore-forming rods. The organism grew optimally at 30–37 °C and at pH 6.0–8.0. Based on 16S rRNA gene sequence analysis, strain SY1T is a member of the genus Sphingobacterium; Sphingobacterium multivorum JCM 21156T was the nearest relative (98.5 % sequence similarity). The predominant fatty acids of strain SY1T were iso-C15 : 0 (32.9 %), C16 : 0 (10.9 %) and summed feature 3 (iso-C15 : 0 2-OH and/or C16 : 1 ω7c; 24.1 %). The DNA G+C content was 38.5 mol%. The low level of DNA–DNA relatedness (2.2 %) to S. multivorum JCM 21156T in combination with differential morphological and biochemical properties demonstrated that strain SY1T (=KCTC 22131T=CGMCC 1.6855T) should be classified as representing a novel species of the genus Sphingobacterium for which the name Sphingobacterium siyangense sp. nov. is proposed.