Two heterotrophic, aerobic, yellow-pigmented, Gram-negative, non-gliding bacteria, designated AKS293T and AKS432T, isolated from a red alga, were analysed using a polyphasic taxonomic approach. 16S rRNA gene sequence analysis revealed that the novel strains were affiliated to the genus Lacinutrix, a member of the family Flavobacteriaceae, showing sequence similarities of 96.1–96.4 % with respect to the type strain of Lacinutrix copepodicola. The two novel isolates shared 99.5 % 16S rRNA gene sequence similarity and 55.0 % DNA–DNA relatedness. They grew optimally at 17.5 °C and pH 6.5. The main cellular fatty acids of strain AKS293T were iso-C15 : 0, iso-C15 : 0 3-OH and iso-C16 : 0 3-OH, while those of strain AKS432T were anteiso-C15 : 0, iso-C15 : 0, iso-C15 : 1 and iso-C15 : 0 3-OH. In both cases, the major isoprenoid quinone was MK-6. The DNA G+C contents were 34.7 and 37.0 mol% for strains AKS293T and AKS432T, respectively. The phylogenetic evidence, phenotypic data and DNA–DNA hybridization results support the differentiation of strains AKS293T and AKS432T from each other and from their closest relative, L. copepodicola DJ3T. Therefore, strains AKS293T and AKS432T represent two novel species, for which the names Lacinutrix algicola sp. nov. and Lacinutrix mariniflava sp. nov. are proposed, respectively. The type strain of L. algicola sp. nov. is AKS293T (=KCCM 42313T=JCM 13825T) and the type strain of L. mariniflava sp. nov. is AKS432T (=KCCM 42306T=JCM 13824T). An emended description of the genus Lacinutrix is also proposed.
The taxonomic position of a strain isolated from kerosene-contaminated soil in Hungary and formerly misidentified as Brevundimonas vesicularis was examined using a polyphasic approach. The isolate, designated CHB-20pT, could be clearly assigned to the genus Chryseobacterium (family Flavobacteriaceae) on the basis of 16S rRNA gene sequence similarity. Strain CHB-20pT, a moderate oil degrader, was a Gram-negative, aerobic, mesophilic microbe with a temperature optimum of 28–30 °C. Predominant fatty acids were iso-C15 : 0, summed feature 3 (comprising C16 : 1 ω7c and/or iso-C15 : 0 2-OH) and iso-C17 : 0 3-OH. Menaquinone-6 (MK-6) was the predominant respiratory quinone; MK-5 was present as a minor component. The almost complete 16S rRNA gene sequence of strain CHB-20pT shared 94–97 % similarity with sequences of the type strains of species of the genus Chryseobacterium. DNA–DNA relatedness between strain CHB-20pT and its closest relative, Chryseobacterium caeni, was lower than 46 %. Moreover, several diagnostic phenotypic properties distinguished strain CHB-20pT from C. caeni. On the basis of biochemical, chemotaxonomic and genotypic data, isolate CHB-20pT represents a novel species within the genus Chryseobacterium, Chryseobacterium hungaricum sp. nov.; the type strain is CHB-20pT (=NCAIM B2269T=DSM 19684T).