A novel yellow-pigmented, Gram-negative, slightly halophilic, catalase-positive, oxidase-negative, obligately aerobic bacterium, designated strain YIM-C338T, was isolated from saline soil from the Qaidam Basin in north-west China. Cells were non-sporulating, non-motile, short rods, predominantly occurring singly. Coccoid bodies and slightly curved rod-shaped cells of varying length developed in older cultures. Growth occurred with 0.5–8 % (w/v) NaCl (optimally with 1–3 %, w/v), at pH 6.0–9.0 (optimally at pH 7.0) and at 4–37 °C (optimally at 28 °C). The major cellular fatty acids were C16 : 0, C18 : 0, C16 : 1 ω7c, C18 : 1 ω7c, iso-C13 : 0 3-OH and iso-C15 : 1. MK-6 was the only respiratory quinone. Non-diffusible carotenoid pigments were produced. Flexirubin-type pigments were absent. The genomic DNA G+C content was 42.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain YIM-C338T was a member of the genus Salinimicrobium, having sequence similarities of 97.3 and 94.0 % with respect to Salinimicrobium xinjiangense BH206T and Salinimicrobium catena HY1T. The combination of the phylogenetic data, phenotypic characteristics and chemotaxonomic differences supported the view that strain YIM-C338T represents a novel species of the genus Salinimicrobium, for which the name Salinimicrobium terrae sp. nov. is proposed. The type strain is YIM-C338T (=DSM 17865T =CGMCC 1.6308T). An emended description of the genus Salinimicrobium is also provided.
Two novel bacterial strains, designated DOKDO 025T and DOKDO 023T, were isolated on Dokdo Island, Korea, from the rhizosphere of the brown alga Ecklonia kurome. The strains were subjected to a polyphasic taxonomy study and were found to be Gram-negative, aerobic, rod-shaped, non-motile and orange-coloured. The isolates shared 96.3 % 16S rRNA gene sequence similarity. They showed 93.8–95.6 % 16S rRNA gene sequence similarity with respect to members of the genus Muricauda in the family Flavobacteriaceae, but formed a distinct phyletic line. Moreover, the cellular appendages reported for all Muricauda species were absent from strains DOKDO 025T and DOKDO 023T. The predominant cellular fatty acids of strain DOKDO 025T were iso-C15 : 0, iso-C15 : 1 and one with an equivalent chain-length of 13.565 and those of strain DOKDO 023T were iso-C15 : 0, iso-C15 : 1 and iso-C17 : 0 3-OH. The DNA G+C content of strains DOKDO 025T and DOKDO 023T were 59.5 and 66.5 mol%, respectively, higher than any values found in recognized members of the family Flavobacteriaceae. The major respiratory quinone was MK-6. On the basis of evidence from the polyphasic study, strains DOKDO 025T and DOKDO 023T represent two novel species in a new genus, Croceitalea gen. nov., for which the names Croceitalea eckloniae sp. nov. (the type species) and Croceitalea dokdonensis sp. nov. are proposed. The type strain of Croceitalea eckloniae sp. nov. is DOKDO 025T (=KCCM 42309T =JCM 13827T) and that of Croceitalea dokdonensis sp. nov. is DOKDO 023T (=KCCM 42308T =JCM 13826T).
Two novel marine, heterotrophic, gliding, Gram-negative, aerobic and orange-pigmented strains were investigated by using a polyphasic approach. 16S rRNA gene sequence analysis indicated an affiliation of the novel isolates to the family Flavobacteriaceae of the phylum Bacteroidetes. The level of sequence similarity between the strains studied and their closest relatives, the recognized Zobellia species, ranged from 93.2 to 93.9 %. Phylogenetic evidence, supported by several differences in phenotypic characteristics between the novel isolates and Zobellia species such as the absence of nitrate reductase, agarase and gelatinase activities together with the ability to produce acid from galactose, lactose and melibiose, allowed their assignment to a new genus and species, for which the name Kriegella aquimaris gen. nov., sp. nov. is proposed. The type strain of Kriegella aquimaris is KMM 3665T (=KCTC 22188T =DSM 19886T).
A Gram-negative, rod-shaped, oxidase-positive, aerobic, non-motile and orange-pigmented bacterial strain, containing flexirubin-type pigments, designated H8T, was isolated from raw cow's milk in Israel. 16S rRNA gene sequence analysis indicated that the isolate should be placed in the genus Chryseobacterium (family Flavobacteriaceae, phylum Bacteroidetes). The levels of 16S rRNA gene sequence similarity between strain H8T and the type strains of described Chryseobacterium species were 97.5 % or lower. Strain H8T grew at 5–37 °C and with 0–3.0 % NaCl. The dominant cellular fatty acids were iso-15 : 0, iso-17 : 0 3-OH, iso-17 : 1ω9c and summed feature 3 (comprising iso-15 : 0 2-OH and/or 16 : 1ω7c). On the basis of phenotypic properties and phylogenetic distinctiveness, the milk isolate H8T is classified as a member of a novel species in the genus Chryseobacterium, for which the name Chryseobacterium oranimense sp. nov. (type strain H8T =LMG 24030T =DSM 19055T) is proposed.