The taxonomic position of a Gram-stain-positive, endospore-forming bacterium, strain KS16-9T, isolated from a marine sediment sample collected from Kovalam, Kanyakumari coastal region of the Indian Ocean, India, was analysed by a polyphasic approach. Strain KS16-9T had typical phenotypic characteristics and chemotaxonomic features (menaquinones, fatty acids and lipids) that were consistent with the genus Bacillus . omparative analysis of the 16S rRNA gene sequence of the strain with previously published Bacillus type strains confirmed that it belongs to the genus Bacillus and is moderately related to Bacillus persicus B48T (98.42 % similarity), followed by Bacillus foraminis CV53T (97.67 %) and Bacillus rigiliprofundi (97.61 %). Other species in the genus Bacillus shared <97.6 % 16S rRNA gene sequence similarity. Strain KS16-9T contained MK-7 as the predominant menaquinone, meso-diaminopimelic acid as the diagnostic cell-wall diamino acid, iso-C15 : 0 and anteiso-C15 : 0 as major fatty acids, and diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine as the major phospholipids. The DNA G+C content of strain KS16-9T was 45.4 mol%. Based on data from this polyphasic taxonomic study, strain KS16-9T represents a novel species of the genus Bacillus , for which the name Bacillus maritimus sp. nov. is proposed. The type strain is KS16-9T (=MTCC 12305T=DSM 100413T=KCTC 33834T).
A Gram-stain-positive, aerobic bacterial strain, designated Y2A20T, with peritrichous flagella was isolated from the top layer saline–alkaline soil, Hangjin Banner, Inner Mongolia, northern China. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain Y2A20T formed a stable clade with ‘ Lysinibacillus jejuensis ’ N2-5T. Strain Y2A20T shared highest 16S rRNA gene sequence similarity with ‘ L. jejuensis ' N2-5T (97.4 %), but lower 16S rRNA gene sequence similarities with all other type strains (<97.0 %). The major polar lipids of strain Y2A20T were diphosphatidylglycerol, phosphatidylglycerol, phosphatidyethanolamine, two unknown aminophospholipids and three unknown phospholipids. Menaquinone-7 was the predominant menaquinone, while iso-C15 : 0, iso-C16 : 0 and iso-C17 : 0 were the major cellular fatty acids. Its genomic DNA G+C content was 39.0 mol%. DNA–DNA hybridization revealed that strain Y2A20T showed 26±5 % genomic DNA relatedness with its closest relative, ‘ L. jejuensis ’ N2-5T. The results of physiological and biochemical tests allowed the discrimination of strain Y2A20T from its phylogenetic relatives. Lysinibacillus alkalisoli sp. nov. is therefore proposed with Y2A20T (=CGMCC 1.15760T=KCTC 33825T) as the type strain.
A Gram-staining-positive, motile, endospore-forming, rod-shaped bacterium, designated NS3012T, was isolated from Myeolchi-jeotgal, a traditional Korean high-salt-fermented anchovy and was characterized using a polyphasic taxonomic approach. Comparative 16S rRNA gene sequence analysis showed that strain NS3012T belongs to the genus Virgibacillus in the family Bacillaceae of the phylum Firmicutes . The 16S rRNA gene sequence analysis showed that strain NS3012T was closely related to Virgibacillus halotolerans WS-4627T (98.1 %), Virgibacillus oceani MY11T (96.6 %) and Virgibacillus byunsanensis ISL-24T (96.5 %). The chemotaxonomic properties of strain NS3012T were consistent with those of the genus Virgibacillus : the quinone system with MK-7 as the predominant menaquinone and anteiso-C15 : 0 and anteiso C17 : 0 as the major cellular fatty acids; the cell-wall peptidoglycan type was based on meso-diaminopimelic acid. The major polar lipid was diphosphatidylglycerol. The G+C content of the genomic DNA was 36.8 mol%. On the basis of phylogenetic inference, and chemotaxonomic and other phenotypic properties, strain NS3012T is clearly differentiated from closely related species of the genus Virgibacillus and represents a novel species in this genus, for which the name Virgibacillus jeotgali sp. nov. is proposed. The type strain is NS3012T (=KEMB 9001-125T=JCM 16522T).