A novel actinomycete, designated strain KC-035T, was isolated from soil collected from Krung Ching Waterfall National Park, Nakhon Si Thammarat Province, Thailand. Its taxonomic position was determined using a polyphasic approach. The strain had morphological and chemotaxonomic properties typical of members of the genus Streptomyces : flexuous spore chain; ll-diaminopimelic acid in the cell-wall peptidoglycan; MK-9(H8), MK-9(H6) and MK-9(H4) as menaquinones; diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside as phospholipids; anteiso-C15 : 0, C16 : 0, iso-C16 : 0, iso-C15 : 0 and iso-C14 : 0 as major cellular fatty acids; and DNA G+C content of 72 mol%. 16S rRNA gene sequence analysis revealed that strain KC-035T showed high similarity to Streptomyces albiflavescens n20T (99.16 %) and Streptomyces siamensis KC-038T (98.43 %) as well as formed a monophyletic clade with them in the phylogenetic tree. On the basis of comparison of phenotypic properties and the low level of DNA–DNA relatedness, strain KC-035T could be distinguished from its closely related Streptomyces species and is considered to represent a novel species of the genus Streptomyces , for which the name Streptomyces krungchingensis sp. nov. is proposed. The type strain is KC-035T (=NBRC 110087T=KCTC 29503T=TISTR 2402T).
A novel actinomycete, designated strain m16T, was isolated from a soil sample collected from the tropical rain forest of Xishuangbanna, a prefecture in Yunnan Province, south-west China, and characterized by using polyphasic taxomomy. Cells were aerobic and Gram-reaction-positive, and spore chains were observed to be of the helical type, with elliptical spores and smooth spore surfaces. The novel strain grew over a temperature range of 15–35 °C, at pH 5.0–11.0 and in the presence of 0–3 % (w/v) NaCl. The DNA G+C content of strain m16T was 70.0 mol%. The main fatty acids were iso-C16 : 0 (29.3 %), iso-C15: 0 (15.4 %) and anteiso-C15:0 (14.6 %), and the predominant menaquinones were MK-9(H6), MK-9(H8) and MK-9(H4). Comparative 16S rRNA gene sequence analysis showed that strain m16T was most closely related to Streptomyces jiujiangensis KCTC 29262T (98.7 %), Streptomyces panaciradicis KACC 17632T (98.7 %), Streptomyces rhizophilus NBRC 108885T (98.5 %), Streptomyces shenzhenensis DSM 42034T (98.4 %), Streptomyces graminisoli JR-19T (98.4 %) and Streptomyces gramineus JR-43T (98.3 %). Phylogenetic, chemotaxonomic and phenotypic analyses indicated that strain m16T represents a novel species within the genus Streptomyces , for which the name Streptomyces fuscichromogenes is proposed. The type strain is m16T (=CGMCC 4.7110T=KCTC 29195T).
A Gram-stain-positive, oxidase-negative, catalase-positive isolate forming sporangium-like globular bodies, isolated from the rare earth mine of Bayan Obo in China and designated strain KC 266T, was subjected to a comprehensive taxonomic study. Comparative 16S rRNA gene sequence analysis revealed that strain KC 266T represented a novel lineage within the genus Kibdelosporangium and showed highest 16S rRNA gene similarities to Kibdelosporangium philippinense (98.5 %), Kibdelosporangium aridum subsp. largum (98.2 %), Kibdelosporangium aridum subsp. aridum (98.2 %) and Kibdelosporangium phytohabitans (98.0 %). The DNA–DNA relatedness between strain KC 266T and the four species of the genus Kibdelosporangium was less than 60 %. The DNA G+C content of strain KC 266T was 67.9 mol%. The quinone system consisted of major amounts of MK-9(H4) and MK-9(H2), minor amounts of MK-8(H2) and traces of MK-10(H4). The diamino acid of the peptidoglycan was meso-diaminopimelic acid. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylinositol, two unknown phospholipids and one unidentified aminophospholipid. The major cellular fatty acids were iso-C16 : 0, C17 : 1 ω6c, iso-C15 : 0 and iso-C14 : 0. Physiological traits as well as unique traits of the polar lipid profile and the fatty acid pattern distinguished strain KC 266T from the most closely related species. All these results indicate that strain KC 266T represents a novel species of the genus Kibdelosporangium , for which the name Kibdelosporangium metalli sp. nov. is proposed. The type strain is KC 266T (=KCTC 39719T=CCTCC AA 2016002T).
A novel endophytic actinobacterial strain, designated EGI 6500195T, was isolated from fruits of Capparis spinosa. Growth occurred at 10–45 °C (optimum 30 °C), at pH 6–8 (optimum pH 7) and in the presence of 0–1 % (w/v) NaCl. Strain EGI 6500195T shared highest 16S rRNA gene sequence similarity (97.74 %) with Streptomyces vitaminophilus DSM 41686T and less than 97 % sequence similarity with other members of the genus Streptomyces . The diagnostic amino acid in the peptidoglycan was ll-diaminopimelic acid. Whole-cell hydrolysates contained glucose, ribose, fructose and mannose. The predominant menaquinones were MK-9(H6) and MK-9(H8). The polar lipid profile of strain EGI 6500195T included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylinositol, phosphatidylcholine, three unknown phospholipids, an unknown aminophospholipid and an unknown aminolipid. The cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0, iso-C15 : 0, iso-C16 : 0, anteiso-C17 : 1 ω9c, summed feature 4 (iso-C17 : 1 I and/or anteiso-C17 : 1 B) and iso-C17 : 1 ω9c. The DNA G+C content of strain EGI 6500195T was 74.1 mol%. The level of DNA–DNA relatedness between strain EGI 6500195T and Streptomyces. vitaminophilus DSM 41686T was 14.1±3.5 %. On the basis of the phenotypic, phylogenetic, chemotaxonomic and DNA–DNA hybridization data, strain EGI 6500195T represents a novel species of the genus Streptomyces , for which the name Streptomyces capparidis sp. nov. is proposed. The type strain is EGI 6500195T (=DSM 42145T=JCM 30089T).
Three strains, FXJ8.095T, FXJ8.057 and H201, were isolated from deep-sea hydrothermal plume water collected at the Southwest Indian Ridge at a depth of 2800 m. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolates formed a closely related subcluster within the genus Kocuria . The 16S rRNA gene sequence of strain FXJ8.095T shared 99.90 and 99.60 % similarity with those of strains FXJ8.057 and H201, respectively, and 98.81, 98.75, 98.68 and 98.10 % with those of ‘ Kocuria sediminis ’ JCM 17929, Kocuria flava HO-9041T, Kocuria turfanensis HO-9042T and Kocuria rosea JCM 11614T, respectively. DNA–DNA hybridization values among the three new isolates were higher than 70 %, while the values between each of the isolates and the closely related type strains were well below 70 %. Random amplified polymorphic DNA fingerprint patterns and a combination of physiological and biochemical properties also distinguished the isolates from the related species. The major cellular fatty acids of the isolates were anteiso-C15 : 0 and iso-C15 : 0, the predominant menaquinones were MK-7(H2) and MK-8(H2), and the main polar lipids were diphosphatidylglycerol and phosphatidylglycerol. The DNA G+C contents of strains FXJ8.095T, FXJ8.057 and H201 were 75.6, 72.8 and 70.4 mol%, respectively. Based on the phylogenetic, phenotypic and chemotaxonomic data, we propose to classify the three strains in a novel species named Kocuria oceani sp. nov., with FXJ8.095T (=CGMCC 4.6946T=DSM 24949T) as the type strain.