One hundred and ninety-eight isolates of soil yeasts were isolated from mixed temperate forests in the Czech Republic, and their abundance and distribution in the litter and soil were evaluated using amplicon sequencing of soil fungal communities. Abundant taxa with no close identified hits were selected for further characterization as potential novel species of yeasts. Phylogenetic analyses using sequences of the D1/D2 domain, the ITS region and RPB1 and TEF1 genes support the recognition of the following three novel species belonging to the subphylum Pucciniomycotina, class Microbotryomycetes: Leucosporidium krtinense f.a. sp. nov. (type strain PYCC 6879T=KT96T=CBS 14304T=DSM 101892T), Yurkovia mendeliana sp. nov. (type strain PYCC 6884T=KT152T=CBS 14273T=DSM 101889T) and Libkindia masarykiana sp. nov. (type strain PYCC 6886T=KT310T=CBS 14275T=DSM 101891T). Since the latter two novel taxa cannot be assigned to existing genera, two new genera, Libkindia gen. nov. and Yurkovia gen. nov., are also described.
Three strains (14Y260T, 14Y268 and 14Y276) of xylose-assimilating yeasts were isolated from decayed wood and soil collected in West Java in Indonesia. A phylogenetic analysis was performed based on the sequences of the D1/D2 domains of LSU, SSU and EF-1α, and the three strains were found to belong to the genus Pichia. The morphological, biochemical, physiological and chemotaxonomic characteristics indicated that these strains were distinct from other closely related species. Strains 14Y260T, 14Y268 and 14Y276 belonged to the Pichia clade and represent a novel species, named Pichia chibodasensis sp. nov. ; The type strain is 14Y260T (=NBRC 111569T=InaCC Y1042T).
A novel basidiomycetous yeast was isolated from the waste deposit of the attine ant Acromyrmex lundii (Hymenoptera: Formicidae). The field colony was located in Santurce town, Santa Fe province, Argentina. The description of the novel species was based on strain LLU043T. Analysis of the D1/D2 domains of the LSU rRNA gene sequences in GenBank demonstrated that strain LLU043T, belongs to the Rhodosporidiobolus clade and is closely related to Rhodosporidiobolus lusitaniae and Rhodosporidiobolus colostri with 97 % similarity to the two species. The novel species differs from R . lusitaniae and R. colostri in some physiological characteristics such as the lack of assimilation of cellobiose, salicin, succinate, citrate and ethylamine. The name Rhodosporidiobolus geoffroeae sp. nov. is proposed, with LLU043T (=CBS 12828T=CBMAI 1618T) as the type strain.