1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 22, Issue 3

Biochemical Characterization and Antigenic Relationship of subsp. Freundt and subsp. (Edward) Freundt Free

Abstract

Forty-seven bovine, caprine, and ovine strains were studied. Fifteen of these were subsp. Freundt; the remaining 32 were previously designated as subsp. (Edward) Freundt, (Wroblewski) Freundt, or Turkish type C. Cultures of these strains were obtained from various laboratories abroad and from the Plum Island Animal Disease Laboratory repository; they included representatives of species and serotypes previously reported. The purity of all 47 cloned strains was checked by a combination of differential inhibitory methods, cloning, characterization, and serological techniques. The strains were tested for glucose fermentation, tetrazolium reduction, arginine decarboxylation, film and spots formation, hemolysis of sheep erythrocytes, inhibitory effect of 0.02% methylene blue, and methylene blue reduction. All subsp. subsp. , and related strains were biochemically and serologically compared with the other strains and with representatives of a recent classification of the entire group of caprine and ovine mycoplasmas. All of the 15 subsp. strains gave similar biochemical reactions. Among the 32 test strains, 2 of sheep origin designated Ghaleh Morghi-16 and S-5-64 were previously misidentified as ; 4 other strains were identified as Turkish C type. All of these strains were identified as belonging to caprine and ovine group 8. The 32 strains were divided into 2 serogroups. One group was comprised of 5 strains, including the type strain of subsp. (strain PG3), and the other group contained 27 strains, including a large number previously identified as subsp. . All of the strains in the latter group showed an antigenic relationship to subsp. whereas the 5 strains of the first group were serologically distinct. The 32 strains including in these 2 groups and the 15 subsp. strains had no antigenic relationship to or to 7 new groups of caprine and ovine Mycoplasmas. It is significant that some of the group 8 strains were isolated from goats and sheep in countries free of contagious bovine pleuropneumonia. Their antigenic identity to subsp. that serious problems might arise if diagnosis of such cultures were made solely on a serological basis.

  • Published Online:
Copyright © 1972 International Association of Microbiological Societies
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-22-3-155
1972-07-01
2024-05-04
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/22/3/ijs-22-3-155.html?itemId=/content/journal/ijsem/10.1099/00207713-22-3-155&mimeType=html&fmt=ahah

References

  1. Al-Aubaidi J. M., Fabricant J. 1968; Technics for the isolation of mycoplasma from cattle. Cornell Vet. 58:555
     [Google Scholar]
  2. Al-Aubaidi J. M., Fabricant J. 1971; Characterization and classification of bovine mycoplasma. Cornell Vet. 61:490
     [Google Scholar]
  3. Al-Aubaidi J. M., Fabricant J. 1971; Methods for the purification of mixed cultures of mycoplasma. Cornell Vet. 61:559
     [Google Scholar]
  4. Argaman M., Razin S. 1969; Antigenic properties of mycoplasma organisms and membranes. J. Gen. Microbiol. 55:45
     [Google Scholar]
  5. Arisoy F., Etheridge J. R., Foggi A., Erdag O. 1970; Contagious caprine pleuropneumonia. A study of caprine mycoplasma strains using a cross protection test in mice. Pendik Vet. Kontr. Arastirma Enst. Derg. 2:2
     [Google Scholar]
  6. Barber T. L., Yedloutschnig R. J. 1970; Mycoplasma infections of goats. Cornell Vet. 28:297
     [Google Scholar]
  7. Brack M. Von. 1966; Mycoplasma bei jungen Mahnenspringerm (Ammotragus lervia). Berlin. Muenchen. Tierarztl. Wochenschr. 79:169
     [Google Scholar]
  8. Clyde W. A. 1964; Mycoplasma species identification based upon growth inhibition by specific antisera. J. Immunol. 92:958
     [Google Scholar]
  9. Cottew G. S., Watson W. A., Arisoy F., Erdag O., Buckley L. S. 1968; Differentiation of M. agalactiae from other mycoplasma of sheep and goats. J. Comp. Pathol. 78:275
     [Google Scholar]
  10. Cottew, Watson W. A., Erdag O., Arisoy F. 1969; Mycoplasma of caprine pleuropneumonia in Turkey and their relationship to other mycoplasma of goats and M. mycoides var. mycoides. J. Comp. Pathol. 79:541
     [Google Scholar]
  11. Edward D. G. ff. 1953; Organisms of the pleuropneumonia group causing disease in goats. Vet. Rec. 65:873
     [Google Scholar]
  12. Edward D. G. ff. 1960; Letters to the editor. Int. Bull. Bacteriol. Nomenci. Taxon. 10:256
     [Google Scholar]
  13. Edward D. G. ff., Freundt E. A. 1969 Classification of the mycoplasmatales. 147–200 Hayflick L. the mycoplasmatales and the L-phase of bacteria Appleton-Century-Crofts; New York:
     [Google Scholar]
  14. El-Nasri M. 1967; Mycoplasma from contagious caprine pleuropneumonia. Ann. N.Y. Acad. Sci. 143:298
     [Google Scholar]
  15. El-Nasri M. 1971 Studies on M. mycoides var. mycoides and M. mycoides var. capri. I. Crosspathogenicity and cross-protection test. FAO/OIE/OAU Fourth Expert Panel on Contagious Bovine Pleuropneumonia Paris, France:
     [Google Scholar]
  16. Griffin R. M. 1969; Antigenic relationships among strains of Mycoplasma mycoides var. mycoides, M. capri and M. laidlawii revealed by complement fixation test. J. Gen. Microbiol. 57:131
     [Google Scholar]
  17. Hudson J. R., Cottew G. H., Adler H. E. 1967; Diseases of goats caused by mycoplasma. A review of the subject with some new findings. Ann. N.Y. Acad. Sci. 143:287
     [Google Scholar]
  18. Jonas A., Barber T. L. 1969; Mycoplasma mycoides var. capri isolated from a goat in Connecticut. J. Infec. Dis. 119:126
     [Google Scholar]
  19. Laws L. 1956; A pleuropneumonia-like organism causing peritonitis in goats. Aust. Vet. J. 32:326–329
     [Google Scholar]
  20. Lemcke R. M. 1964; The serological differentiation of mycoplasma strains (pleuropneumonia-like organisms) from various sources. J. Hyg. 62:199
     [Google Scholar]
  21. Lemcke R. M., Shaw J. E., Marmion B. P. 1965; Related antigens in Mycoplasma pneumoniae and Mycoplasma mycoides var. mycoides. Aust. J. Exp. Biol. Med. Sci. 43:761
     [Google Scholar]
  22. Lindley E. P., Abdulla A. E. D. 1969; Some notes on the host specificity of the etiological agents of contagious caprine pleuropneumonia and contagious bovine pleuropneumonia. Bull. Epizoot. Dis. Afr. 17:153
     [Google Scholar]
  23. Longley E. O. 1940; Contagious pleuropneumonia of goats. Indian J. Vet. Sci. 10:127
     [Google Scholar]
  24. Longley E. O. 1951; Contagious caprine pleuropneumonia. A study of the disease in Nigeria. Colon. Res Pubi. No. 7
    [Google Scholar]
  25. Neimark H. E. 1970; Division of mycoplasma into subgroups. J. Gen. Microbiol. 63:249
     [Google Scholar]
  26. Perreau P., Gayt P., Monnier J. 1969; The fluorescent antibody method for identifying mycoplasmas. Application for the diagnosis of contagious bovine pleuropneumonia. Rev. Elevage Med. Vet. Pays Trop. 22:481
     [Google Scholar]
  27. Provost A., Villemot J.M., Perreau P., Queval R., Borredon C. 1967; Some concepts on the antigenic relationships of different mycoplasma both within and outside the group. Ann. N.Y. Acad. Sci. 143:38
     [Google Scholar]
  28. Razin S. 1968; Mycoplasma taxonomy studied by electrophoresis of cell proteins. J. Bacteriol. 96:687–694
     [Google Scholar]
  29. Rodwell A. W. 1967; The nutrition and metabolism of mycoplasma: progress and problem. Ann. N.Y. Acad. Sci. 143:88
     [Google Scholar]
  30. Rodwell A. W. 1969; The supply of cholesterol and fatty acids for the growth of mycoplasma. J. Gen. Microbiol. 58:29
     [Google Scholar]
  31. Shirlaw J. F. 1949; Studies on contagious pleuropneumonia of the goat of India. Indian J. Vet. Sci. 19:181
     [Google Scholar]
  32. Smith G.R. 1971; The use of mice in research on contagious bovine pleuropneumonia. Presented at Fourth Meeting of the FAO/OIE/OAU Expert Panel on Contagious Bovine Pleuropneumonia
     [Google Scholar]
  33. Taylor-Robinson D., Purcell R. H., Wong D. C., Chanock R. M. 1966; A color test for the measurement of antibody to certain mycoplasma species based upon the inhibition of acid production. J. Hyg. 64:91
     [Google Scholar]
  34. Tully J. G., Razin S. 1968; Physiological and serological comparisons among strains of Mycoplasma granulorum and Mycoplasma laidlawii. J. Bacteriol. 95:1504
     [Google Scholar]
  35. Turner A. W. 1960; Letters to the editors. Int. Bull. Bacteriol. Nomenci. Taxon. 10:256
     [Google Scholar]
  36. Villemot J. M., Provost A. 1959; Recherches immunologiques sur la peripneumonia. V. Relations antigeniques entre Mycoplasma mycoides var. mycoides, M. mycoides var. capri et al autres micro organisms du genre mycoplasma. Rev. Elevage Med. Vet. Pays Trop. 12:251
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-22-3-155
Loading
Biochemical Characterization and Antigenic Relationship of Mycoplasma mycoides subsp. mycoides, Freundt and Mycoplasma mycoides subsp. capri (Edward) Freundt
Int J Syst Evol Microbiol 22, 155 (1972); https://doi.org/10.1099/00207713-22-3-155
/content/journal/ijsem/10.1099/00207713-22-3-155
/content/journal/ijsem/10.1099/00207713-22-3-155
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error