1887

Abstract

One hundred and two strains of aerobic, gram-negative bacteria, isolated mainly from drugs and cosmetics, were tested for catabolic products after incubation in liquid media containing single amino acids. Breakdown products from L-arginine, lysine, and ornithine were identified as their -heptafluoryl butyl esters by gas-liquid chromatographic techniques. A Finnigan model 1015 D gas chromatograph/mass spectrometer/PDP-8E data system was used to identify trace amounts of catabolites after amino acid substrates were inoculated with bacteria. The derivatization reaction was monitored in like manner. The Finnigan data system may be used to identify catabolic compounds rapidly and thus provide a means for bacterial characterization and identification.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-27-3-179
1977-07-01
2024-05-05
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/27/3/ijs-27-3-179.html?itemId=/content/journal/ijsem/10.1099/00207713-27-3-179&mimeType=html&fmt=ahah

References

  1. Blair J. E., Lennette E. H., Truant J. P.ed 1974 Manual of clinical microbiology. American Society for Microbiology. Washington, D.C.:
    [Google Scholar]
  2. Bodily H. L., Updyke E. L., Mason J. O.ed 1970 Diagnostic procedures for bacterial, mycotic, and parasitic infections. , 5th. American Public Health Association, Inc.; Washington, D.C.:
    [Google Scholar]
  3. Brooks K., Sodeman T. 1974; Clinical studies on a transformation test for identification of Acinetobacter (Mima and Herelled). Appl. Microbiol. 27:1023–1026
    [Google Scholar]
  4. Center for Disease Control 1972 The identification of unusual pathogenic gram-negative bacteria (Elizabeth 0. King). Atlanta, Ga:
    [Google Scholar]
  5. Edwards P. R., Ewing W. H. 1972 Identification of Enterobacteriaceae,. , 3rd. Burgess Publishing Co.; Minneapolis:
    [Google Scholar]
  6. Evans J. R., Gilden M. M., Bruch C. W. 1972; Methods for isolating and identifying objectionable gram-negative bacteria and endotoxins from topical products. J. Soc. Cosmet. Chern. 23:549–564
    [Google Scholar]
  7. Falkow S. 1958; Activity of lysine decarboxylase as an aid in the identification of Salmonella and shigellae. Am. J. Clin. Pathol. 29:598–600
    [Google Scholar]
  8. Fugate K. J., Hansen L. B., White O. 1971; Analysis of Clostridium botulinum toxigenic types A, B and E for fatty and carbohydrate content. Appl. Microbiol. 21:470–475
    [Google Scholar]
  9. Gilardi G. L. 1973; Nonfermentative gram-negative bacteria encountered in clinical specimens. Antonie van Leeuwenhoek J. Microbiol. Serol. 39:229–242
    [Google Scholar]
  10. Hugh R., Leifson E. 1953; The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various gram-negative bacteria. J. Bacteriol. 66:24–26
    [Google Scholar]
  11. Kakimoto T., Shibatani T., Nishimura N., Chibata I. 1971; Enzymatic production of L-citrulline by Pseudomonas putida. Appl. Microbiol. 22:992–999
    [Google Scholar]
  12. Lambert M. A., Moss C. W. 1973; Use of gas chromatography for detecting ornithine and lysine decarboxylase activity in bacteria. Appl. Microbiol. 26:517–520
    [Google Scholar]
  13. Moeller V. 1955; Simplified tests for some amino-acid decarboxylases and for the arginine dihydrolase system. Acta Pathol. Microbiol. Scand. 36:158–172
    [Google Scholar]
  14. Morris D. R., Pardee A. B. 1966; Multiple pathways of putrescine biosynthesis in Escherichia coli. J. Biol. Chern. 241:3129–3135
    [Google Scholar]
  15. Moss C. W., Lambert M. A., Cherry W. B. 1972; Use of gas chromatography for determining catabolic products of arginine by bacteria. Appl. Microbiol. 23:889–893
    [Google Scholar]
  16. Pinter M., Bende I. 1967; Computer analysis of Acinetobacter Iwoffi (Moraxella Iwoffi) and Acinetobacter anitratus (Moraxella glucidolytica) strains. J. Gen. Microbiol. 46:267–272
    [Google Scholar]
  17. Ramos R., Stalon V., Pierard A., Wiame J. M. 1967; The specialization of the two ornithine carbamolyl-transferases of Pseudomonas. Biochim. Biophys. Acta 139:98–106
    [Google Scholar]
  18. Taylor J. J., Whitby J. L. 1964; Pseudomonas pyocyanea and the arginine dihydrolase system. J. Clin. Pathol. 17:122–125
    [Google Scholar]
  19. Yamamoto K., Sato T., Tosa T., Chibata I. 1974; Continuous production of L-citrulline by immobilized Pseudomonas putida cells. Biotechnol. Bioeng. 16:1589–1599
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-27-3-179
Loading
/content/journal/ijsem/10.1099/00207713-27-3-179
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error