1887

Abstract

Good separation of bacterial cell wall amino acids, including the isomers of diaminopimelic acid, was obtained by two-dimensional thin-layer chromatography, using commercial cellulose-coated aluminum sheets with isopropanol-acetic acid-water (75:10:15, vol/vol) and methanol-pyridine-10 N hydrochloric acid-water (64:8:2:14, vol/vol) as the first and second solvents, respectively.

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/content/journal/ijsem/10.1099/00207713-29-1-56
1979-01-01
2024-05-04
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References

  1. Becker B., Lechevalier M. P., Gordon R. E., Lechevalier H. A. 1964; Rapid differentiation between Nocardia and Streptomyces by paper chromatography of whole-cell hydrolysates. Appl. Microbiol 12:421–423
    [Google Scholar]
  2. Keddie R. M., Cure G. L. 1977; The cell wall composition and distribution of free mycolic acids in named strains of coryneform bacteria and in isolates from various natural sources. J. Appl. Bacteriol 42:229–252
    [Google Scholar]
  3. Partridge S. M., Westall R. G. 1948; Filter paper partition paper chromatography of sugars. 1. General description and application to the quantitative analysis of sugars in apple-juice, egg-white and foetal blood of sheep. Biochem. J 42:238–250
    [Google Scholar]
  4. Rhuland L. E., Work E., Denman R. F., Hoare D. S. 1955; The behaviours of the isomers of ae- diaminopimelic acid on paper chromatograms. J. Am. Chem. Soc 77:4844–4846
    [Google Scholar]
  5. Schleifer K. H., Handler O. 1972; Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol. Rev 36:407–477
    [Google Scholar]
  6. Staneck J. C., Roberts G. D. 1974; Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl. Microbiol 28:226–231
    [Google Scholar]
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