1887

Abstract

Polynucleotide sequence relationships among strains of were assessed by analysis of deoxyribonucleic acid-deoxyribonucleic acid homo and heteroduplexes with endonuclease S1. The results showed that strains isolated from different subjects form a very tight group, with deoxyribonucleic acid homology levels ranging between 93 and 100%. Physiological tests of 23 strains included 30 different substrates. Results were remarkably uniform. All 23 strains grew better anaerobically with added H and CO than aerobically. However, colony size was greater on blood agar but not Trypticase soy (BBL Microbiology Systems, Cockeysville, Md.)-yeast extract agar with an atmosphere of 4% O with added H and CO than with anaerobic incubation. The data suggest that these strains are sufficiently closely related to justify their inclusion in a single discrete species, but their appropriate generic classification remains to be resolved.

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1979-10-01
2024-04-28
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References

  1. Cowan S. T. 1974; Cowan and Steel’s manual for the identification of medical bacteria. Cambridge University Press; London:
    [Google Scholar]
  2. Crosa J. H., Brenner D. J., Falkow S. 1973; Use of a single-strand specific nuclease for analysis of bacterial and plasmid deoxyribonucleic acid homo- and heteroduplexes. J. Bacterid 115:904–911
    [Google Scholar]
  3. Evans C. A., Mattern K. L. 1978; Individual differences in the bacterial flora of the skin of the forehead: Peptococcus saccharolyticus. J. Invest. Dermatol 71:152–153
    [Google Scholar]
  4. Evans C. A., Mattern K. L., Hallam S. L. 1978; Isolation and identification of Peptococcus saccharolyticus from human skin. J. Clin. Microbiol 7:261–264
    [Google Scholar]
  5. Foubert E. L. Jr, Douglas H. C. 1948; Studies on the anaerobic micrococci. I. Taxonomic considerations. J. Bacterid 56:25–34
    [Google Scholar]
  6. Holdeman L. V., Cato E. P., Moore W. E. C. ed 1977; Anaerobe laboratory manual. , 4th ed. Virginia Polytechnic Institute and State University; Blacksburg:
    [Google Scholar]
  7. Kelly R. B., Cozzarelli N. R., Deutscher M. P., Lehman I. R., Kornberg A. 1970; Enzymatic synthesis of deoxyribonucleic acid. XXXII. Replication of duplex deoxyribonucleic acid by polymerase at a single strand break. J. Biol. Chem 245:39–45
    [Google Scholar]
  8. Maniatis T., Jeffrey A., Kleid D. G. 1975; Nucleotide sequence of the rightward operator of phage X. Proc. Natl. Acad. Sci. U.S.A 72:1184–1188
    [Google Scholar]
  9. Rogosa M. 1974; Genus I Peptococcus Kluyver and vanNiel 1936. p 518–522 In Buchanan R. E., Gibbons N. E. ed Bergey’s manual of determinative bacteriology. , 8th ed. The Williams & Wilkins Co; Baltimore:
    [Google Scholar]
  10. Schleifer K. H., Nimmerman E. 1973; Peptidogly- can types of strains of the genus Peptococcus. Arch. Mikrobiol 93:245–258
    [Google Scholar]
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