1887

Abstract

A taxonomic study of Gardner and Dukes was undertaken to determine relationships between this organism and members of other genera. The methods utilized included Adansonian analysis, deoxyribonucleic acid (DNA)-DNA hybridizations, electron microscopy, and biochemical analysis of cell envelopes. By numerical analysis, all 78 clinical isolates and reference strains examined were related to each other at a similarity level exceeding 95%. No subspecies or biovars were observed. DNA-DNA hybridizations showed no genetic relationship between and members of the genera , and . Also, no relationship was observed between and CDC DF-1. In the absence of existing genera with genetic features compatible with , we propose the new genus for inclusion of organisms presently designated as either or is defined to include catalase- and oxidase-negative, gram-negative to gram-variable bacteria with laminated cell walls which produce acetic acid as the major end product of fermentation. The type species of is (Gardner and Dukes) comb. nov. Due to the unusual cell wall of this organism, this new genus is not presently assignable to a family.

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1980-01-01
2024-03-29
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References

  1. Brenner D. J., Fanning G. R., Rake A. V., Johnson K. E. 1969; Batch procedure for thermal elution of DNA from hydroxyapatite. Anal. Biochem 28:447–459
    [Google Scholar]
  2. Buchanan R. E., Gibbons N. E. ed 1974; Ber- gey’s manual of determinative bacteriology, 8th ed. The Williams & Wilkins Co; Baltimore:
    [Google Scholar]
  3. Burdett I. D. J., Murray R. G. E. 1974; Electron microscope study of septum formation in Escherichia coli strains B and B/r during synchronous growth. J. Bacteriol 119:1039–1056
    [Google Scholar]
  4. Cazzulo J. J. 1973; On the regulatory properties of a halophilic citrate synthase. FEBS Lett 30:339–342
    [Google Scholar]
  5. Criswell B. S., Ladwig C. L., Gardner H. L., Dukes C. D. 1969; Haemophilus vaginalis: vaginitis by inoculation from culture. Obstet. Gynecol 33:195–199
    [Google Scholar]
  6. Criswell B. S., Marston J. H., Stenback W. A., Black S. H., Gardner H. L. 1971; Haemophilus vaginalis 594, a gram-negative organism?. Can. J. Microbiol 17:865–869
    [Google Scholar]
  7. Criswell B. S., Stenback W. A., Black S. H., Gardner H. L. 1972; Fine structure of Haemophilus vaginalis.. J. Bacteriol 109:930–932
    [Google Scholar]
  8. De Ley J., Van Muylem J. 1963; Some applications of deoxyribonucleic acid base composition in bacterial taxonomy. Antonie van Leeuwenhoek J. Microbiol. Serol 29:344–358
    [Google Scholar]
  9. Dunkelberg W. E. 1974; Monograph: a bibliographic review of Corynebacterium vaginale (H. vaginalis).. Atlanta Printing Office; Fort McPherson, Ga:
    [Google Scholar]
  10. Dunkelberg W. E. Jr, McVeigh I. 1969; Growth requirements of Haemophilus vaginalis.. Antonie van Leeuwenhoek J. Microbiol. Serol 35:129–145
    [Google Scholar]
  11. Edmunds P. N. 1962; The biochemical, serological and haemagglutinating reactions of “Haemophilus vaginalis”.. J. Pathol. Bacteriol 83:411–422
    [Google Scholar]
  12. Frampton J., Lee Y. 1964; Is Haemophilus vaginalis a pathogen in the female genital tract?. J. Obstet. Gynaecol. Br. Commonw 71:436–442
    [Google Scholar]
  13. Gardner H. L., Dukes C. D. 1955; Haemophilus vaginalis vaginitis. A newly defined specific infection previously classified “nonspecific” vaginitis. Am. J. Ob-stet. Gynecol 69:962–976
    [Google Scholar]
  14. Greenwood J. R., Pickett M. J. 1979; Salient features of Haemophilus vaginalis.. J. Clin. Microbiol 9:200–204
    [Google Scholar]
  15. Greenwood J. R., Pickett M. J., Martin W. J., Mack E. G. 1977; Haemophilus vaginalis {Corynebacterium vaginale): method for isolation and rapid biochemical identification. Health Lab. Sci 14:102–106
    [Google Scholar]
  16. Jones D., Weitzman P. D. J. 1971; Taxonomic significance of citrate synthase. J. Gen. Microbiol 69:xi
    [Google Scholar]
  17. Josey W. E., Lambe D. W. Jr 1976; Epidemiologic characteristics of women infected with Corynebacterium vaginale (Haemophilus vaginalis).. J. Am. Vener. Dis. Assoc 3:9–13
    [Google Scholar]
  18. Kourilsky P., Leidner J., Tremblay G. Y. 1971; DNA-DNA hybridization of filters at low temperature in the presence of formamide or urea. Biochimie 53:1111–1114
    [Google Scholar]
  19. Leopold S. 1953; Heretofore undescribed organism iso lated from the genitourinary system. U.S. Armed Forces Med. J 4:263–266
    [Google Scholar]
  20. Lessel E. F. 1962; Bacterial type cultures of the American Type Culture Collection. I. Int. Bull. Bacteriol. Nomencl. Taxon 12:71–88
    [Google Scholar]
  21. Malone B. H., Schreiber M., Schneider N. J., Holdeman L. V. 1975; Obligately anaerobic strains of Corynebacterium vaginale (Haemophilus vaginalis).. J. Clin. Microbiol 2:272–275
    [Google Scholar]
  22. Marmur J. 1961; A procedure for isolation of deoxyri bonucleic acid from micro-organisms. J. Mol. Biol 3:208–218
    [Google Scholar]
  23. Morrison W. R., Smith L. M. 1964; Preparation of fatty acid methyl esters and dimethylacetals from lipids with boron fluoride-methanol. J. Lipid Res 5:600–608
    [Google Scholar]
  24. Moss C. W., Dunkelberg W. E. Jr 1969; Volatile and cellular fatty acids of Haemophilus vaginalis.. J. Bacteriol 100:544–546
    [Google Scholar]
  25. Murray R. G. E. 1962; Fine structure and taxonomy of bacteria. p 119–144 In Ainsworth G. C., Sneath P. H. A. ed Microbial classification. Twelfth Symposium of the Society for General Microbiology. University Press; Cambridge:
    [Google Scholar]
  26. Newman M. G., Sutter V. L., Pickett M. J., Blachman U., Greenwood J. R., Grinenko V., Citron D. 1979; Detection, identification, and comparison of Cap- nocytophaga Bacteroides ochraceus, and DF-1. J. Clin. Microbiol 10:557–562
    [Google Scholar]
  27. Pheifer T. A., Forsyth P. S., Durfee M. A., Pollock H. M., Holmes K. K. 1978; Nonspecific vagin-itis. Role of Haemophilus vaginalis and treatment with metronidazole. N. Engl. J. Med 298:1429–1434
    [Google Scholar]
  28. Pontefract R. D., Bergeron G., Thatcher F. S. 1969; Mesosomes in Escherichia coli.. J. Bacteriol 97:367–375
    [Google Scholar]
  29. Reyn A., Birch-Andersen A., Lapage S. P. 1966; An electron microscope study of thin sections of Haemophilus vaginalis (Gardner and Dukes) and some possibly related species. Can. J. Microbiol 12:1125–1136
    [Google Scholar]
  30. Rietschel E. Th., Gottert H., Luderitz O., Westphal O. 1972; Nature and linkages of the fatty acids present in the lipid-A component of Salmonella lipo- polysaccharides. Eur. J. Biochem 28:166–173
    [Google Scholar]
  31. Salton M. R. J. 1964; The bacterial cell wall. p 1–293 American Elsevier Publishing Co., Inc; New York:
    [Google Scholar]
  32. Schildkraut C. L., Marmur J., Doty P. 1962; Determination of the base composition of deoxyribo-nucleic acid from its buoyant density in CsCl. J. Mol. Biol 4:430–443
    [Google Scholar]
  33. Smaron M. F., Vice J. L. 1977; Immunological and chemical characterization of the extracellular antigens from Corynebacterium vaginale.. Infect. Immun 18:356–362
    [Google Scholar]
  34. Vickerstaff J. M., Cole B. C. 1969; Characteriza tion of Haemophilus vaginalis, Corynebacterium cer- vicis, and related bacteria. Can. J. Microbiol 15:587–594
    [Google Scholar]
  35. Weissbach A., Hurwitz J. 1959; The formation of 2-keto-3-deoxyheptonic acid in extracts of Escherichia coli B. I. Identification. J. Biol. Chem 234:705–709
    [Google Scholar]
  36. Weitzman P. D. J. 1978; Anomalous citrate synthase from Thermus aquaticus.. J. Gen. Microbiol 106:383–386
    [Google Scholar]
  37. Westphal O., Jann K. 1965; Bacterial lipopolysac- charides. Extraction with phenol-water and further applications of the procedure. Methods Carbohydr. Chem 5:83–91
    [Google Scholar]
  38. Zinnemann K., Turner G. C. 1963; The taxonomic position of "Haemophilus vaginalis” (Corynebacterium vaginale). J. Pathol. Bacteriol 85:213–219
    [Google Scholar]
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