Simple Method for Determination of Deoxyribonucleic Acid Relatedness by Thermal Elution in Hydroxyapatite Microcolumns
Lachance, Marc-andré.,, 30, 433-436 (1980),
doi = https://doi.org/10.1099/00207713-30-2-433,
publicationName = Microbiology Society,
issn = 1466-5026,
abstract= A convenient method has been devised to chromatograph simultaneously numerous samples of reassociated deoxyribonucleic acid under thermoregulated conditions. Small amounts of hydroxyapatite (0.2-ml bed) were used, and the effluent volumes for separating single-stranded deoxyribonucleic acid from deoxyribonucleic acid duplexes were 1.5 ml each. The method was comparable in reliability and reproducibility to other well-established techniques, but it combined improvements in ease of operation, in rapidity, and in simplicity, which are desirable in large taxonomic studies.,
language=,
type=