1887

Abstract

When polyacrylamide gel electrophoretic profiles of soluble proteins of seven strains were compared with those of seven coryneform and coryneform-like species, the arthrobacters were delineated from the latter species. Comparisons were made from a composite gel by calculating similarity coefficients. When was used as a reference, similarity coefficients for the other species ranged from 20.7 to 34.5, but the similarity coefficients for the other genera tested were only 7.9 to 18.5. In addition to a separation of arthrobacters from other coryneforms by this method, and had a similarity coefficient of approximately 95, which set these two apart from the other species studied and furthermore suggested that they represent one species. Gel electrophoresis of soluble proteins may provide a good alternative to the use of rod to coccoid transition for identifying arthrobacters since it is faster and simpler to perform than chemical analyses of cell wall components.

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1980-04-01
2024-04-20
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References

  1. Brewer J. M., Peace A. J., Ashworth R. B. 1974 Experimental techniques in biochemistry Prentice-Hall, Inc; Englewood Cliffs, N. J:
    [Google Scholar]
  2. Buchanan R. E., Gibbons N. E. ed 1974 Bergey’s manual of determinative bacteriology The Williams & Wilkins Co; Baltimore:
    [Google Scholar]
  3. Conn H. J., Dimmick I. 1947; Soil bacteria similar in morphology to Mycobacterium Corynebacterium. J. Bacteriol 54:291–303
    [Google Scholar]
  4. Cummins C. S. 1962; Chemical composition and antigenic structure of cell walls of Corynebacterium, My-cobacterium, Nocardia, Actinomyces and Arthrobacter. J. Gen. Microbiol 28:35–50
    [Google Scholar]
  5. Holland A. A., Gray T. R. G., Luscombe B. M. 1972; Immunodiffusion and fluorescent antibody methods to distinguish rod and coccoid forms of a species of Arthrobacter. Antonie van Leeuwenhoek J. Microbiol. Serol 38:169–176
    [Google Scholar]
  6. Katznelson H., Mason A. 1962; Serological relationships among species of Arthrobacter. Can J. Microbiol 8:588–591
    [Google Scholar]
  7. Maizel J. V. 1971; Polyacrylamide gel electrophoresis of viral proteins. p 179–246 In Maramorosh K., Koprowski H. ed Methods in virology. vol 5 Academic Press Inc; New York:
    [Google Scholar]
  8. Sneath P. H. A. 1957; The application of computers to taxonomy. J. Gen. Microbiol 17:201–226
    [Google Scholar]
  9. Stackebrandt E., Fiedler F. 1979; DNA-DNA homology studies among strains of Arthrobacter Brevibacterium. Arch. Microbiol 120:289–295
    [Google Scholar]
  10. Veldkamp H. 1970; Saprophytic coryneform bacteria. Annu. Rev. Microbiol 24:209–240
    [Google Scholar]
  11. Yamada K., Komagata K. 1970; Taxonomic studies on coryneform bacteria. II. Principal amino acids in the cell wall and their taxonomic significance. J. Gen. Appl. Microbiol 16:103–113
    [Google Scholar]
  12. Yamada K., Komagata K. 1970; Taxonomic studies on coryneform bacteria. III. DNA base composition of coryneform bacteria. J. Gen. Appl. Microbiol 16:215–224
    [Google Scholar]
  13. Yamada K., Komagata K. 1972; Taxonomic studies on coryneform bacteria. V. Classification of coryneform bacteria. J. Gen. Appl. Microbiol 18:417–431
    [Google Scholar]
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