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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 30, Issue 2

Emendation of the Genus Kudriavzev and Comparison of Species by Deoxyribonucleic Acid Reassociation, Mating Reaction, and Ascospore Ultrastructure Free

Abstract

The genus Kudriavzev has been emended to include all nitrate-negative, multilateral budding yeast species that form unconjugated persistent asci with roughened spheroidal ascospores and have Q-7 ubiquinone in the electron transport system. var. , and var. are assigned to this genus as Kudriavzev, (van der Walt) comb. nov., var. (Phaff et al.) comb. nov., and var. (Phaff et al.) comb. nov., respectively. Additionally, one new species, , is described. The type strain of is NRRL Y-7552 (=CBS 5459). Electron microscopy demonstrated that the ascospore walls of have thick inner and outer layers and that the ascospores of the other species have walls with a thick inner layer and a thin, dense outer layer. With the exception of var. , ascospore surface ornamentation arises from the dense outer layer. Deoxyribonucleic acid reassociation studies and mating tests confirmed the recognition of four species in the genus and showed to be the imperfect state of was identified as the imperfect form of var. and var. showed only 25© deoxyribonucleic acid complementarity, yet intervarietal matings formed viable ascospores. This is the lowest deoxyribonucleic acid relatedness ever shown between strains capable of genetic hybridization, and the implications of this finding are discussed.

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© Society for General Microbiology, 1980
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1980-04-01
2024-04-19
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Emendation of the Genus Issatchenkia Kudriavzev and Comparison of Species by Deoxyribonucleic Acid Reassociation, Mating Reaction, and Ascospore Ultrastructure
Int J Syst Evol Microbiol 30, 503 (1980); https://doi.org/10.1099/00207713-30-2-503
/content/journal/ijsem/10.1099/00207713-30-2-503
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