1887

Abstract

and , two new species isolated from periodontal pockets and the superficially cleaned tooth surface coronal to the gingival margin, from various types of human infections, and from chicken intestinal contents are described. They were obligately anaerobic, gram-negative, nonpigmenting, non-motile, non-spore-forming rods that did not grow well in 10% bile and that fermented carbohydrates. Although we had previously identified many of these strains as members of subsp. biovar (biotype) 3 (Holdeman et al. [ed.], , 4th ed., 1977), in the present study, we found that the strains had no deoxyribonucleic acid homology with the type strains of subsp. or subsp. . The strains also had no deoxyribonucleic acid homology with the type strain of . ATCC 27518, which we deposited as representative of human isolates of subsp. biovar 3, is now identified as a strain of . The type strains of and are ATCC 33573 and ATCC 33574, respectively.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-32-1-125
1982-01-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/32/1/ijs-32-1-125.html?itemId=/content/journal/ijsem/10.1099/00207713-32-1-125&mimeType=html&fmt=ahah

References

  1. Bryant M. P., Robinson I. M., Bouma C., Chu H. 1958; Bacteroides ruminicola n. sp. and the new genus and species Succininimonas amylolytica. Species of succinte acid-producing anaerobic bacteria of the bovine rumen. J. Bacteriol 76:15–23
    [Google Scholar]
  2. Crosa J. H., Brenner D. J., Falkow S. 1973; Use of single-strand-specific nuclease for analysis of bacterial and plasmid deoxyribonucleic acid homo- and heterodu-plexes. J. Bacteriol 115:904–911
    [Google Scholar]
  3. Cummins C. S., Johnson J. L. 1971; Taxonomy of the clostridia: wall composition and DNA homologies in Clostridium butyricum and other butyric acid-producing clostridia. J. Gen. Microbiol 67:33–46
    [Google Scholar]
  4. Evans F. O. Jr, Sydnor J. B., Moore W. E. C., Moore G. R., Manwaring J. L., Brill A. H., Jackson R. T., Hana S., Skaar J. S., Holdeman L. V., Fitz-Hugh G. S., Sande M. A., Gwaltney J. M. Jr 1975; Sinusitis of the maxillary antrum. N. Engl. J. Med 293:735–739
    [Google Scholar]
  5. Holdeman L. V., Cato E. P., Burmeister J. A., Moore W. E. C. 1980; Descriptions of Eubacterium timidum sp. nov., Eubacterium brachy sp. nov., and Eubacterium nodatum sp. nov. isolated from human periodontitis. Int. J. Syst. Bacteriol 30:163–169
    [Google Scholar]
  6. Holdeman L. V., Cato E. P., Moore W. E. C. 1977; Anaerobe laboratory manual. , 4. Anaerobe Laboratory Virginia Polytechnic Institute and State University; Blacksburg, Va:
    [Google Scholar]
  7. Holdeman L. V., Moore W. E. C. 1974; Genus I. Bacteroides Castellani and Chalmers 1919. 959385–404 Buchanan R. E., Gibbons N. E. Bergey’s manual of determinative bacteriology, 8. The Williams & Wilkins Co; Baltimore:
    [Google Scholar]
  8. Johnson J. L. 1981; Genetic characterization. 450–472 Gerhardt P. Manual of methods for general microbiology American Society for Microbiology; Washington, D.C:
    [Google Scholar]
  9. Johnson J. L., Cummins C. S. 1972; Cell wall composition and deoxyribonucleic acid similarities among the anaerobic coryneforms, classical propionibacteria, and strains of Arachnia propionica . J. Bacteriol 109:1047–1066
    [Google Scholar]
  10. Loesche W. J., Socransky S. S., Gibbons R. J. 1964; Bacteroides oralis, proposed new species isolated from the oral cavity of man. J. Bacteriol 88:1329–1337
    [Google Scholar]
  11. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J. Mol. Biol 3:208–218
    [Google Scholar]
  12. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J. Mol. Biol 5:109–118
    [Google Scholar]
  13. Moore W. E. C., Hash D. E., Holdeman L. V., Cato E. P. 1980; Polyacrylamide slab gel electrophoresis of soluble proteins for studies of bacterial floras. Appl. Environ. Microbiol 39:900–907
    [Google Scholar]
  14. Wilkins T. D., Thiel T. 1973; A modified broth-disk method for testing the antibiotic susceptibility of anaerobic bacteria. Antimicrob. Agents Chemother 3:350–356
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-32-1-125
Loading
/content/journal/ijsem/10.1099/00207713-32-1-125
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error