1887

Abstract

Whole-cell fatty acid analyses of 79 strains demonstrated that all 79 strains were closely related and were distinct from other pseudomonads whose fatty acid profiles were reported previously. Outer membranes were isolated from seven representative strains, and the protein patterns were compared by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Four major outer membrane proteins were found in each of the strains but not in the outer membranes of 8 other strains or 10 other fish pathogens. The outer membranes of the seven strains studied were immunologically cross-reactive. We suggest that outer membrane protein patterns may be a useful taxonomic tool.

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1983-01-01
2024-03-28
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References

  1. Buchanan T. M., Hildebrand J. F. 1981; Antigen-specific serotyping of Neisseria gonorrhoeae: characterization based upon principal outer membrane protein. Infect. Immun. 32:985–994
    [Google Scholar]
  2. Cullen J., Phillips M. C., Shipley G. G. 1971; The effects of temperature on the composition and physical properties of lipids of P. fluorescens . Biochem. J. 125:733–742
    [Google Scholar]
  3. Dees S. B., Moss C. W. 1975; Cellular fatty acids of Alcaligenes and Pseudomonas species isolated from clinical specimens. J. Clin. Microbiol. 1:414–419
    [Google Scholar]
  4. De Vos P. 1980; Intrageneric and intergeneric similarities of ribosomal RNA cistrons of the genus Pseudomonas and the implications for taxonomy. Antonie van Leeuwen-hoek J. Microbiol. Serol. 46:96
    [Google Scholar]
  5. Hancock I. C., Meadow P. M. 1969; The extractable lipids of Pseudomonas aeruginosa . Biochim. Biophys. Acta 187:366–378
    [Google Scholar]
  6. Hancock R. E. W., Carey A. M. 1979; The outer membrane of Pseudomonas aeruginosa: heat- and β-mercaptoethanol-modifiable proteins. J. Bacteriol. 140:902–910
    [Google Scholar]
  7. Hancock R. E. W., Nikaido H. 1978; Outer membranes of gram-negative bacteria. XIX. Isolation from Pseudomonas aeruginosa and use in reconstitution and definition of the outer membrane permeability barrier. J. Bacteriol. 136:381–390
    [Google Scholar]
  8. Hancock R. E. W., Wieczorek A. A., Mutharia L. M., Poole K. 1982; Monoclonal antibodies against Pseudomonas aeruginosa outer membrane antigens. Isolation and characterization. Infect. Immun. 37:166–171
    [Google Scholar]
  9. Hofstra H., Dankert J. 1980; Major outer membrane proteins: common antigens in Enterobacteriaceae species. J. Gen. Microbiol. 119:123–131
    [Google Scholar]
  10. Kaltenbach C. M., Moss C. W., Weaver R. E. 1975; Cultural and biochemical characteristics and fatty acid composition of Pseudomonas diminuta and Pseudomonas vesiculare . J. Clin. Microbiol. 1:339–344
    [Google Scholar]
  11. Kropinski A. M., Kuzio J., Angus B. L., Hancock R. E. W. 1982; Chemical and chromatographic analysis of lipopolysaccharide from an antibiotic-supersusceptible mutant of Pseudomonas aeruginosa . Antimicrob. Agents Chemother. 21:310–319
    [Google Scholar]
  12. Moss C. W., Samuels S. B., Weaver R. E. 1972; Cellular fatty acid composition of selected Pseudomonas species. Appl. Microbiol. 24:596–598
    [Google Scholar]
  13. Muroga K. 1978; Red spot disease of eels. Fish Pathol. 13:35–39
    [Google Scholar]
  14. Nakai T., Muroga K., Wakabayashi H. 1980; Serological properties of Pseudomonas anguilliseptica in agglutination. Bull. Jpn. Soc. Sci. Fish. 47:699–703
    [Google Scholar]
  15. Overbeeke N., Lugtenberg B. 1980; Major outer membrane proteins of Escherichia coli strains of human origin. J. Gen. Microbiol. 121:373–380
    [Google Scholar]
  16. Palleroni N. J., Kunisawa R., Contopoulou R., Doudoroff M. 1973; Nucleic acid homologies in the genus Pseudomonas . Int. J. Syst. Bacteriol. 23:333–339
    [Google Scholar]
  17. Skerman V. B. D., McGowan V., Sneath P. H. A. 1980; Approved lists of bacterial names. Int. J. Syst. Bacteriol. 30:225–420
    [Google Scholar]
  18. Wakabayashi H., Egusa S. 1972; Characteristics of a Pseudomonas sp. from an epizootic of pond-cultured eels (Anguilla japonica). Bull. Jpn. Soc. Sci. Fish. 38:577–587
    [Google Scholar]
  19. Wilkinson S. G., Galbraith L., Lightfoot G. A. 1973; Cell walls, lipids and lipopolysaccharides of Pseudomonas species. Eur. J. Biochem. 33:158–174
    [Google Scholar]
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