RT Journal Article SR Electronic(1) A1 EMOD, ISTVAN A1 SOUBIGOU, PASCAL A1 TONG, NGUYEN T. A1 KEIL, BORIVOJ A1 RICHARD, CLAUDEYR 1983 T1 Assignment of “Achromobacter iophagus” Strain I.029 to Vibrio alginolyticus Chemovar iophagus JF International Journal of Systematic and Evolutionary Microbiology, VO 33 IS 3 SP 451 OP 459 DO https://doi.org/10.1099/00207713-33-3-451 PB Microbiology Society, SN 1466-5034, AB Strain CIP 82.01 of Vibrio alginolyticus and strain I.029 (previously designated “Achromobacter iophagus”) were compared. Strain I.029 produces a collagenase of high specific activity (Achromobacter collagenase; EC 3.4.24.8). Collagenase production is induced in strain CIP 82.01 by collagen or macromolecular fragments of collagen in a manner similar to collagenase induction in strain I.029; caseinolytic proteinase is constitutive. In this study we demonstrate that both strains also produce a constitutive extracellular endonuclease. Collagenases from both strains cleave either native collagen in its helical region or a similar synthetic peptide; both enzymes are inhibited by ethylenediamine tetraacetate, but not by diisopropyl fluorophosphate. The collagenase subunit (molecular weight, 35,000) of strain CIP 82.01 is similar in amino acid composition to the subunit of the strain I.029 enzyme, although some of the aspartic and threonine residues in strain CIP 82.01 are replaced by glutamic and serine residues in strain I.029. Surface radioiodination followed by two-dimensional electrophoresis showed that there are quantitative differences in the major outer membrane proteins of the two strains. Strains CIP 82.01 and I.029 differ qualitatively in resistance to ampicillin and carbenicillin, in cellobiose fermentation, in ornithine decarboxylase activity, and in halophilism. We propose that strain I.029, which was originally designated A. iophagus, be included within the species V. alginolyticus, but that this organism be distinguished from other strains of this species by the designation Vibrio alginolyticus chemovar iophagus, with the corresponding collagenase designated “iophagus collagenase” (EC 3.4.24.8)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/00207713-33-3-451