1887

Abstract

Deoxyribonucleic acid (DNA) was extracted from mycobacterial cells by gentle lysis (sodium dodecyl sulfate and pronase treatment) of wall-deficient forms obtained after -cycloserine and glycine treatment. DNA from CIP 8139 (= ATCC 35219) (T = type strain) was H-labeled by nick-translation. DNA relatedness studies (S1 nuclease method) showed that the seven strains studied form a single DNA hybridization group which is less than 25% related to 16 other species of the genus The guanine-plus-cytosine content of DNA was 68 mol%, and the guanine-plus-cytosine contents of , and DNAs were 64 to 65 mol%.

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1984-10-01
2024-03-28
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References

  1. Baess I. 1974; Isolation and purification of deoxyribonucleic acid from mycobacteria. Acta Pathol. Microbiol. Scand. Sect. B 82:780–784
    [Google Scholar]
  2. Baess I. 1976; DNA-DNA hybridization in mycobacteria. 225–229 Freekrsen E., Tarnok I., Thumim J. H. Genetics of the ActinomycetalesProceedings of the International Colloquium at the Forschunginstitut Borstel Gustav Fisher Verlag; Stuttgart:
    [Google Scholar]
  3. Baess I. 1979; Deoxyribonucleic acid relatedness among species of slowly growing mycobacteria. Acta Pathol. Microbiol. Scand. Sect. B 87:221–226
    [Google Scholar]
  4. Baess I. 1982; Deoxyribonucleic acid relatedness among species of rapidly growing mycobacteria. Acta Pathol. Microbiol. Scand. Sect. B 90:371–375
    [Google Scholar]
  5. Baess I., Magnusson M. 1982; Classification of Mycobacterium simiae by means of comparative reciprocal intradermal sensitin testing on guinea pigs and deoxyribonucleic acid hybridization. Acta Pathol. Microbiol. Scand. Sect. B 90:101–107
    [Google Scholar]
  6. Baess I., Mansa B. 1978; Determination of genome size and base ratio on deoxyribonucleic acid from mycobacteria. Acta Pathol. Microbiol. Scand. Sect. B 86:309–312
    [Google Scholar]
  7. Baess I., Weis Bentzon M. 1978; Deoxyribonucleic acid hybridization between different species of mycobacteria. Acta Pathol. Microbiol. Scand. Sect. B 86:71–76
    [Google Scholar]
  8. Bradley S. G. 1973; Relationships among mycobacteria and nocardiae based upon deoxyribonucleic acid association. J. Bacteriol 113:645–651
    [Google Scholar]
  9. Bradley S. G., Enquist L. W., Scribner H. E. III 1976; Heterogeneity among deoxyribonucleic sequences of Actinomycetales . 207–227 Freekrsen E., Tarnok I., Thumim J. H. Genetics of the ActinomycetalesProceedings of the International Colloquium at the Forschungsinstitut Borstel Gustav Fisher Verlag; Stuttgart:
    [Google Scholar]
  10. Brenner D. J. 1981; Introduction to the family Enterobacteriaceae . 1105–1127 Starr M. P., Stolp H., Triiper H. G., Balows A., Schlegel H. G. The prokaryotes. A handbook on habitats, isolation, and identification of bacteria 2 Springer-Verlag; Heidelberg:
    [Google Scholar]
  11. Brenner D. J., McWhorter A. C., Leete Knudson J. K., Steigerwalt A. G. 1982; Escherichia vulneris: a new species of Enterobacteriaceae associated with human wounds. J. Clin. Microbiol 15:1133–1140
    [Google Scholar]
  12. Crosa J. H., Brenner D. J., Falkow S. 1973; Use of a single strand-specific nuclease for analysis of bacterial and plasmid deoxyribonucleic acid homo- and heteroduplexes. J. Bacteriol 115:904–911
    [Google Scholar]
  13. Grimont P. A. D., Popoff M. Y., Grimont F., Coynault C., Lemelin M. 1980; Reproducibility and correlation study of three deoxyribonucleic acid hybridization procedures. Curr. Microbiol 4:325–330
    [Google Scholar]
  14. Gross W. M., Wayne L. G. 1970; Nucleic acid homology in the genus Mycobacterium . J. Bacteriol 104:630–634
    [Google Scholar]
  15. Höllander R., Pohl S. 1980; Deoxyribonucleic acid base composition of bacteria. Zentralbl. Bakteriol. Parasitenkd. In-fektionskr Hyg. Abt. 1 Orig. Reihe A 246:236–275
    [Google Scholar]
  16. Imaeda T., Barksdale L., Kirchheimer W. F. 1982; Deoxyribonucleic acid of Mycobacterium lepraemurium: its genome size, base ratio, and homology with those of other mycobacteria. Int. J. Syst. Bacteriol 32:456–458
    [Google Scholar]
  17. Imaeda T., Kirchheimer W. F., Barksdale L. 1982; DNA isolated from Mycobacterium leprae: genome size, base ratio, and homology with other related bacteria as determined by optical DNA-DNA reassociation. J. Bacteriol 150:414–417
    [Google Scholar]
  18. Lévy-Frébault V., Rafidinarivo E., Promé J. C., Grandry J., Boisvert H., David H. L. 1983; Mycobacterium fallax sp. nov. Int. J. Syst. Bacteriol 33:336–343
    [Google Scholar]
  19. Mizuguchi Y., Tokunaga T. 1970; Method for isolation of deoxyribonucleic acid from mycobacteria. J. Bacteriol 104:1020–1021
    [Google Scholar]
  20. Owen R. J., Hill L. R., Lapage S. P. 1969; Determination of DNA base composition from melting profiles in dilute buffers. Biopolymers 7:503–516
    [Google Scholar]
  21. Rastogi N., David H. L. 1981; Ultrastructural and chemical studies on wall-deficient forms, spheroplasts and membrane vesicles from Mycobacterium aurum . J. Gen. Microbiol 124:71–79
    [Google Scholar]
  22. Wayne L. G., Gross W. M. 1968; Base composition of deoxyribonucleic acid isolated from mycobacteria. J. Bacteriol 96:1915–1919
    [Google Scholar]
  23. Wayne L. G., Gross W. M. 1968; Isolation of deoxyribonucleic acid from mycobacteria. J. Bacteriol 95:1481–1482
    [Google Scholar]
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