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Volume 34, Issue 4

Phylogenetic Relationships Among the Methylotrophic Methane-Producing Bacteria and Emendation of the Family Free

Abstract

The phylogenetic relationships among seven strains of methylotrophic methane-producing bacteria were determined by ribosomal ribonucleic acid hybridization and deoxyribonucleic acid homology techniques. The strains tested had deoxyribonucleic acid guanine-plus-cytosine contents of 39 to 43 mol% and represented a diversity of phenotypic characteristics. Our results indicate that these strains should be divided into six species within the genera , and The genus includes strains MS and 227, sp. strain TM-1, strain C2A, and strain S-6. The genus is represented by strain Tindari 3, and the genus is represented by strain TMA-10. Despite phenotypic similarities between and , we propose that these species remain in separate genera based on differences in ribosomal ribonucleic acid homology and fractional differences in midpoint temperatures. The divisions indicated by deoxyribonucleic acid homology experiments complemented the ribosomal ribonucleic acid hybridization results. Phenotypic characteristics were consistent with these phylogenetic divisions; an apparent exception was cell wall composition, which is a conserved trait. had only a thin protein cell wall, but all other strans of previously studied have been reported to have heteropolysaccharide cell walls. We present evidence which indicates that a protein component may be associated with the heteropolysaccharide cell wall of strain 227.

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Copyright © 1984 International Union of Microbiological Societies
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1984-10-01
2024-04-20
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Phylogenetic Relationships Among the Methylotrophic Methane-Producing Bacteria and Emendation of the Family Methanosarcinaceae
Int J Syst Evol Microbiol 34, 444 (1984); https://doi.org/10.1099/00207713-34-4-444
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