1887

Abstract

A molecular approach was used to examine the genetic relatedness of 19 isolates by measuring the extent of deoxyribonucleic acid-deoxyribonucleic acid homology and the fidelity of hybrid duplex molecules. The isolates examined were divided into two groups based on the results of hybridization tests. The members of genogroup 1, consisting of isolates from , and host plants, exhibited high levels of homology (67.4 to 94.1%) with strain ArI4, an isolate from . Isolates from , and and isolate AirI2 from did not exhibit significant homology (less than 39%) with strain ArI4. None of the strains showed a high degree of homology with strain EuIla (less than 33%), an isolate from . Among the isolates not belonging to genogroup 1, subgroupings seemed to exist, as evidenced by a very high level of homology (97%) between two isolates from , strains D11 and the G2, but a low level of homology (27%) between other strains and strain G2. Thermal stability studies of the hybrid deoxyribonucleic acids which showed high levels of homology revealed an average mismatch of 3%, whereas the low-homology duplexes exhibited about 5% mismatch. The genome molecular weights of two probe strains, strains ArI4 and EuIla, were 8.3 × 10 and 6.0 × 10, respectively.

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1985-04-01
2024-03-29
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References

  1. An C. S., Wills J. H., Riggsby W. S., Mullin B. C. 1983; Deoxyribonucleic acid base composition of 12 Frankia isolates. Can. J. Bot 61:2859–2862
    [Google Scholar]
  2. Baker D. 1982; A cumulative listing of isolated frankiae, the symbiotic nitrogen-fixing actinomycetes. Actinomycetes 17:35–42
    [Google Scholar]
  3. Baker D., Pengelly W. L., Torrey J. G. 1981; Immunological analysis of relationships among the isolated frankiae (Acti-nomycetales). Int. J. Syst. Bacteriol 31:148–151
    [Google Scholar]
  4. Baker D., Torrey J. G. 1979; The isolation and cultivation of actinomycetous root nodule endophytes. 38–56 Gordon J. C., Wheeler C. T., Perry D. A. Symbiotic nitrogen fixation in the management of temperate forests Forestry Research Laboratory, Oregon State University; Corvallis:
    [Google Scholar]
  5. Baker D., Torrey J. G. 1980; Characterization of an effective actinorhizal microsymbiont, Frankia sp. Avcll (Acti-nomycetales). Can. J. Microbiol 26:1066–1071
    [Google Scholar]
  6. Becking J. H. 1974; Frankiaceae. 701–708 Buchanan R. E., Gibbons N. E. Bergy’s manual of determinative bacteriology, 8. The Williams & Wilkins Co; Baltimore:
    [Google Scholar]
  7. Berry A., Torrey J. G. 1979; Isolation and characterization in vitro and in vivo of an actinomycetous endophyte from Alnus rubra Bong. 69–83 Gordon J. C., Wheeler G. T., Perry D. A. Symbotic nitrogen fixation in the management of temperate forests Forest Research Laboratory, Oregon State University; Corvallis:
    [Google Scholar]
  8. Bradley S. G., Enquist L. W., Scribner H. E. III 1978; Heterogenetiy among deoxyribonucleotide sequences of Acti-nomycetales. 207–224 Freerksen E. J., Tarnok I., Thumin J. H. Genetics of the Actinomycetales Gustav Fischer Verlag; Stuttgart:
    [Google Scholar]
  9. Brenner D. J., Fanning G. R., Johnson K. E., Citarella R. V., Falkow S. 1969; Polynucleotide sequence relationship among members of the Enterobacteriaceae . J. Bacteriol 98:637–650
    [Google Scholar]
  10. Britten R. J., Kohne D. E. 1968; Repeated sequences in DNA. Science 161:529–540
    [Google Scholar]
  11. Callaham D., De Tredici P., Torrey J. G. 1978; Isolation and cultivation in vitro of the actinomycete causing root nodulation in Comptonia . Science 199:899–902
    [Google Scholar]
  12. Goodfellow M., Minnikin D. E. M. 1981; Classification of nocardioform bacteria. 7–16 Schaal K. P., Pulverer G. Actinomycetes. Proceedings of the Fourth International Symposium on Actinomycete Biology Gustav Fischer Verlag; New York:
    [Google Scholar]
  13. Hutton J. R. 1977; Renaturation kinetics and thermal stability of DNA in aqueous solutions of formamide and urea. Nucleic Acids Res 4:3537–3555
    [Google Scholar]
  14. Johnson J. L. 1973; Use of nucleic acid homologies in the taxonomy of anaerobic bacteria. Int. J. Syst. Bacteriol 23:308–315
    [Google Scholar]
  15. Knittel M. D., Black C. H., Sandine W. E., Fraser D. K. 1968; Use of normal probability paper in determining thermal melting values of deoxyribonucleic acid. Can. J. Microbiol 14:239–245
    [Google Scholar]
  16. Lalonde M., Calvert E. H. 1979; Production of Frankia hyphae and spores as an infective inoculant for Alnus species. 95–100 Gordon J. C., Wheeler C. T., Perry D. A. Symbiotic nitrogen fixation in the management of temperate forests Forest Research Laboratory, Oregon State University; Corvallis:
    [Google Scholar]
  17. Lauer G. D., Roberts T. M., Klotz L. C. 1977; Determination of the nuclear DNA content of Saccharomyces cerevisiae and implications for the organization of DNA in yeast chromosomes. J. Mol. Biol 114:507–526
    [Google Scholar]
  18. Lechevalier M. P., Baker D., Horriere F. 1983; Physiology, chemistry, serology and infectivity of two Frankia isolates from Alnus incarna (L) subsp. rugosa . Can. J. Bot 61:2826–2833
    [Google Scholar]
  19. Lechevalier M. P., Lechevalier H. A. 1984; Taxonomy of Frankia . 575–582 Ortiz-Ortiz L., Bojalil L. J., Yakoleff V. Biological, biochemical and biomedical aspects of Actinomycetes Academic Press, Inc; New York:
    [Google Scholar]
  20. Lewin B. 1980 Gene expression 2 John Wiley & Sons, Inc; New York:
    [Google Scholar]
  21. Martinson H. G., Wagenear E. B. 1974; Thermal elution chromatography and the resolution of nucleic acids on hydroxylapatite. Anal. Biochem 61:144–154
    [Google Scholar]
  22. Martinson H. G., Wagenear E. B. 1977; Thermal elution chromatography of nucleic acids on hydroxyapatite. Biochim. Biophys. Acta 474:445–455
    [Google Scholar]
  23. McConaughy R. L., Laird C. D., McCarthy B. J. 1969; Nucleic acid reassociation in formamide. Biochemistry 8:3289–3297
    [Google Scholar]
  24. Pearson W. R., Davidson E. H., Britten R. J. 1977; A program for least square analysis of reassociation and hybridization data. Nucleic Acids Res 4:1727–1737
    [Google Scholar]
  25. Rigby P.W., Dieckmann M., Rhodes C., Berg P. 1977; Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase 1. J. Mol. Biol 113:237–251
    [Google Scholar]
  26. Riggsby W. S., Torres-Bauza L. J., Wills J. W., Townes T. M. 1982; DNA content, kinetic complexity and the ploidy question in Candida albicans . Mol. Cell. Biol 2:853–862
    [Google Scholar]
  27. Ruvkun G. B., Ausubel F. M. 1980; Interspecies homology of nitrogenase genes. Proc. Natl. Acad. SciU.S.A 77:191–195
    [Google Scholar]
  28. Schleifer K. H., Stackebrandt E. 1983; Molecular systematics of procaryotes. Annu. Rev. Microbiol 37:143–187
    [Google Scholar]
  29. Smith M. J., Hough B. R., Chamberlin M. E., Davidson E. H. 1974; Repetitive and non-repetitive sequences in sea urchin heterogeneous nuclear RNA. J. Mol. Biol 85:103–126
    [Google Scholar]
  30. Wetmur J. G., Davidson N. 1968; Kinetics of renaturation of DNA. J. Mol. Biol 31:349–370
    [Google Scholar]
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