1887

Abstract

More than 200 mycoplasma isolates recovered from a variety of contaminated cell cultures from 1968 to the present were found to belong to a distinct serological group (serogroup 38). An analysis of representative strains of this collection showed that they possessed all of the characteristics of organisms belonging to the genus . These organisms were capable of fermenting glucose and of hydrolyzing arginine, and they required cholesterol or serum for growth. These organisms were unusual in that they possessed an organized terminal structure or tip, a morphological structure which has been found in at least six other species in the genus . These organisms were also serologically distinct from all previously established species in the genus and the genus . On the basis of these findings and other morphological, biological, and serological characteristics of the strains recovered, we propose that mycoplasmas with these properties belong to a new species, sp. nov. Strain HRC 70-159 (= ATCC 25960) is the type strain of this species.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-35-3-285
1985-07-01
2024-04-24
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/35/3/ijs-35-3-285.html?itemId=/content/journal/ijsem/10.1099/00207713-35-3-285&mimeType=html&fmt=ahah

References

  1. Aluotto B. B., Wittier R. G., Williams C. O., Faber J. E. 1970; Standardized bactériologie techniques for the characterization of Mycoplasma species. Int. J. Syst. Bacteriol 20:35–58
    [Google Scholar]
  2. Barile M. F. 1973; Mycoplasmal contamination of cell cultures: mycoplasma-virus-cell culture interactions. 131–172 Fogh J. Contamination of cell cultures Academic Press, Inc; New York:
    [Google Scholar]
  3. Barile M. F. 1979; Mycoplasma-tissue cell interactions. 425–474 Tully J. G., Whitcomb R. F. The mycoplasmas 2 Human and animal mycoplasmas Academic Press, Inc; New York:
    [Google Scholar]
  4. Barile M. F., Del Giudice R. A. 1972 Isolation of mycoplasmas and their rapid identification by plate epiimmunofluorescence165–181 Ciba Foundation Symposium on Pathogenesis of Mycoplasma. Associated Scientific Publishers; Amsterdam:
    [Google Scholar]
  5. Barile M. F., Hopps H. E., Grabowski M. W. 1978; Incidence and source of mycoplasma contamination: a brief review. 35–45 McGarrity G. J., Murphy D. G., Nichols W. W. Mycoplasma infection of cell cultures Plenum Press; New York:
    [Google Scholar]
  6. Barile M. F., Hopps H. E., Grabowski M. W., Riggs D. B., Del Giudice R. A. 1973; The identification and sources of mycoplasmas isolated from contaminated cell cultures. Ann. N.Y. Acad. Sci 225:251–264
    [Google Scholar]
  7. Biberfeld G., Biberfeld P. 1970; Ultrastructural features of Mycoplasma pneumoniae. J. Bacteriol 102:855–861
    [Google Scholar]
  8. Bradbury J. M. 1977; Rapid biochemical tests for characterization of Mycoplasmatales . J. Clin. Microbiol 5:531–534
    [Google Scholar]
  9. Christiansen G., Christiansen G., Freundt E. A. 1985; Lack of genetic relatedness between Mycoplasma alvi and Mycoplasma sualvi Int. J. Syst. Bacteriol 35:210
    [Google Scholar]
  10. Clyde W. A. Jr 1964; Mycoplasma species identification based upon growth inhibition by specific antisera. J. Immunol 92:958–963
    [Google Scholar]
  11. Cole R. M. 1983; Transmission electron microscopy: basic techniques. 43–50 Razin S., Tully J. G. Methods in mycoplasmology 1 Academic Press, Inc; New York:
    [Google Scholar]
  12. Cole R. M., Popkin T. J. 1981; Electron microscopy and ultrastructure of bacteria. 34–51 Gerhard P. Manual of methods for general bacteriology American Society for Microbiology; Washington, D.C.:
    [Google Scholar]
  13. Cunningham S. 1978; NIAID catalog of research reagents. Department of Health, Education and Welfare publication (NIH)78–899 National Institutes of Health; Bethesda, Md.:
    [Google Scholar]
  14. de Harven E. 1973; Identification of tissue culture contaminants by electron microscopy. 205–231 Fogh J. Contamination of cell cultures Academic Press, Inc; New York:
    [Google Scholar]
  15. Del Giudice R. A., Gardella R. S. 1984; Mycoplasma infection on cell culture: effects, incidence, and detection. 104–115 In vitro monograph no. 5. Use and standardization of vertebrate cell cultures Tissue Culture Association; Gaithersburg, Md.:
    [Google Scholar]
  16. Del Giudice R. A., Gardella R. S., Hopps H. E. 1980; Cultivation of formerly noncultivable strains of Mycoplasma hyorhinis . Curr. Microbiol 4:75–80
    [Google Scholar]
  17. Del Giudice R. A., Hopps H. E. 1978; Microbiological methods and fluorescent microscopy for the direct demonstration of mycoplasma infection of cell cultures. 67–69 McGarrity G. J., Murphy D. G., Nichols W. W. Mycoplasma infection of cell cultures Plenum Press; New York:
    [Google Scholar]
  18. Del Giudice R. A., Robillard N. F., Carski T. R. 1976; Immunofiuorescence identification of mycoplasma on agar by use of incident illumination. J. Bacteriol 93:1205–1209
    [Google Scholar]
  19. Edward D. G. ff. 1947; A selective medium for pleuro-pneumonia-like organisms. J. Gen. Microbiol 1:238–243
    [Google Scholar]
  20. Gourlay R. N., Wyld S. G., Leach R. H. 1977; Mycoplasma alvi a new species from the bovine intestinal and urogenital tracts. Int. J. Syst. Bacteriol 27:89–96
    [Google Scholar]
  21. Hatanaka M., Del Giudice R. A., Long C. 1975; Adenine formation from adenosine by mycoplasmas: adenosine phosphorylase activity. Proc. Natl. Acad. SciU.S.A. 72:1401–1405
    [Google Scholar]
  22. Hu P. C., Cole R. M., Huang Y. S., Graham J. A., Gardner D. E., Collier A. M., Clyde W. A. Jr 1982; Mycoplasma pneumoniae infection: role of a surface protein in the attachment organelle. Science 216:313–315
    [Google Scholar]
  23. Kirchhoff H., Rosengarten R., Lotz W., Fischer M., Lopatta D. 1984; Flask-shaped mycoplasmas: properties and path-ogenicity for man and animals. Isr. J. Med. Sci. 20:848–853
    [Google Scholar]
  24. Long C. W., Del Giudice R. A., Gardella R. S., Hatanaka M. 1977; Uracil phosphoribosyl transferase activity of mycoplasma and infected cell cultures. In Vitro 13:429–433
    [Google Scholar]
  25. Luft J. H. 1971; Ruthenium red and violet. I. Chemistry, purification, methods of use for electron microscopy and mechanism of action. Anat. Rec 171:347–368
    [Google Scholar]
  26. Manchee R. J., Taylor-Robinson D. 1969; Utilization of neuraminic acid receptors for mycoplasmas. J. Bacteriol 98:914–919
    [Google Scholar]
  27. McGarrity G. J., Carson D. A. 1982; Adenosine phos-phorylase-mediated nucleoside toxicity. Exp. Cell Res 139:199–205
    [Google Scholar]
  28. Razin S., Tully J. G. 1970; Cholesterol requirement of mycoplasmas. J. Bacteriol 107:306–310
    [Google Scholar]
  29. Schildkraut C. L., Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its buoyant density in CaCl. J. Mol. Biol 4:430–433
    [Google Scholar]
  30. Subcommittee on the Taxonomy of Mollicutes 1979; Proposal of minimal standards for descriptions of new species of the class Mollicutes Int. J. Syst. Bacteriol 29:172–180
    [Google Scholar]
  31. Tully J. G., Barile M. F., Edward D. G. ff., Theodore T. S., Ernø H. 1974; Characterization of some caprine mycoplasmas, with proposals for new species, Mycoplasma capricolum and Mycoplasma putrefaciens . J. Gen. Microbiol 85:102–120
    [Google Scholar]
  32. Tully J. G., Taylor-Robinson D., Rose D. L., Cole R. M., Bove J. M. 1983; Mycoplasma genitalium a new species from the human urogenital tract. Int. J. Syst. Bacteriol 33:387–396
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-35-3-285
Loading
/content/journal/ijsem/10.1099/00207713-35-3-285
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error