1887

Abstract

Using deoxyribonucleic acid hybridization (S1 nuclease method), we identified 12 hybridization groups (genospecies) containing 74 strains among 85 strains studied. A total of 28 characters which allowed identification of the genospecies were applied to 266 strains. Of the 12 genospecies, 11 could be unambiguously identified. Genospecies 1 ( sensu stricto) contained eight glucose-oxidizing strains which were unable to grow at 44°C and were isolated from soil, including the type strain of Genospecies 2, which contained 121 strains (mostly glucose oxidizers that were able to grow at 44°C), was named sp. nov. (type strain, strain ATCC 19606). Genospecies 3 contained 15 strains that were able to oxidize glucose and to grow at 41°C but not at 44°C. Genospecies 4, which contained 23 hemolytic and proteolytic strains that were able to utilize -4-aminobutyrate but not -lactate, was named sp. nov. (type strain, strain ATCC 17906). Genospecies 5, which contained 17 strains that were unable to oxidize glucose and able to utilize -lactate and -histidine but not glutarate or azelate, was named sp. nov. (type strain, strain ATCC 17908). Genospecies 6 contained only three hemolytic, proteolytic strains that were unable to utilize -lactate, malonate, or -4-aminobutyrate. Genospecies 7, which contained 23 strains that were unable to grow at 37°C and to oxidize glucose and utilized only a few carbon sources was named sp. nov. (type strain, strain ATCC 17909). A total of 34 strains had the characteristics of genospecies 8/9 (mostly glucose negative; utilized azelate but not Simmons citrate, glutarate, -histidine, -aspartate, -leucine, β-alanine, or 2,3-butanediol). Genospecies 8 was sensu stricto since it contained the type strain of this species. Genospecies 9 could not be differentiated from genospecies 8. Genospecies 10 (four strains), 11 (four strains), and 12 (three strains) were differentiated by their nutritional patterns.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-36-2-228
1986-04-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/36/2/ijsem-36-2-228.html?itemId=/content/journal/ijsem/10.1099/00207713-36-2-228&mimeType=html&fmt=ahah

References

  1. Baumann P., Doudoroff M., Stanier R. Y. 1968; A study of the Moraxella group. II. Oxidative-negative species (genus Acinetobacter). J. Bacteriol. 95:1520–1541
    [Google Scholar]
  2. Beijerinck M. W. 1911; Uber Pigmentbildung bei Essigbakterien. Zentralbl. Bakteriol. Parasitenkd. Infektionskr. Hyg. Abt. 2 29:169–176
    [Google Scholar]
  3. Bonner T. L, Brenner D. J., Neufeld B. R., Britten R. J. 1973; Reduction in the rate of DNA reassociation by sequence divergence. J. Mol. Biol. 81:123–135
    [Google Scholar]
  4. Brenner D. J. 1978; Characterization and clinical identification of Enterobacteriaceae by DNA hybridization. Prog. Clin. Pathol. 7:71–117
    [Google Scholar]
  5. Brenner D. J., Fanning G. R., Rake A. V., Johnson K. E. 1969; Batch procedure for thermal elution of DNA from hydroxyapatite. Anal. Biochem. 28:447–459
    [Google Scholar]
  6. Brenner D. J., McWhorter A. C., Leete Knudson J. K., Steigerwalt A. G. 1982; Escherichia vulneris: a new species of Enterobacteriaceae associated with human wounds. J. Clin. Microbiol. 15:1133–1140
    [Google Scholar]
  7. Brisou B., Richard C., Lenriot A. 1972; Intérêt taxonomique de la recherche de la 0-xylosidase chez les Enterobacteriaceae. Ann. Inst. Pasteur (Paris) 123:341–347
    [Google Scholar]
  8. Clowes R. C., Hayes W. 1968 Experiments in microbial genetics. Blackwell Scientific Publications; Oxford:
    [Google Scholar]
  9. Crosa J. H., Brenner D. J., Falkow S. 1973; Use of a single-strand-specific nuclease for analysis of bacterial and plasmid deoxyribonucleic acid homo- and heteroduplexes. J. Bacteriol. 115:904–911
    [Google Scholar]
  10. Escande F., Grimont F., Grimont P. A. D., Bercovier H. 1984; Deoxyribonucleic acid relatedness among strains of Actinobacillus spp. and Pasteurella ureae. Int. J. Syst. Bacteriol. 34:309–315
    [Google Scholar]
  11. Gavini F., Izard D., Leclerc H., Desmonceaux M., Gayral J. P. 1980; Carbon source assimilation tests: comparison between a conventional method and a microtechnic (API), in study of Enterobacteriaceae. Zentralbl. Bakteriol. Parasitenkd. Infektionskr. Hyg. Abt. 1 Orig. Reihe C 1:182–187
    [Google Scholar]
  12. Giammanco G., Buissière J., Toucas M., Brault G., Le Minor L. 1980; Intérêt taxonomique de la recherche de la γ- glutamyltransférase chez les Enterobacteriaceae. Ann. Microbiol. (Paris) 131A:181–187
    [Google Scholar]
  13. Grimont P. A. D. 1984 DNA/DNA hybridization in bacterial taxonomy. 11–19 Sanna A., Morace G.ed New horizons in microbiology Elsevier; Amsterdam:
    [Google Scholar]
  14. Grimont P. A. D., Grimont F., Dulong de Rosnay H. L. C., Sneath P. H. A. 1977; Taxonomy of the genus Serratia. J. Gen. Microbiol. 98:39–66
    [Google Scholar]
  15. Grimont P. A. D., Popoff M. Y., Grimont F., Coynault C., Lemelin M. 1980; Reproductibility and correlation study of three deoxyribonucleic acid hybridization procedures. Curr. Microbiol. 4:325–330
    [Google Scholar]
  16. Hansen W., Schoutens E., Yourassowsky E. 1977; Taxonomy of Acinetobacter·. the usefulness of 0-D-xyloside xylohydrolase for strain differentiation. J. Clin. Pathol. 30:838–841
    [Google Scholar]
  17. Johnson J. L., Anderson R. S., Ordal E. J. 1970; Nucleic acid homologies among oxidase-negative Moraxella species. J. Bacteriol. 101:568–573
    [Google Scholar]
  18. Juni E. 1972; Interspecies transformation of Acinetobacter: genetic evidence for a ubiquitous genus. J. Bacteriol. 112:917–931
    [Google Scholar]
  19. Juni E. 1984 Genus III. Acinetobacter Brisou et Prévôt 1954. 303–307 Krieg N. R., Holt J. G.ed Bergey’s manual of systematic bacteriology 1 The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  20. Lautrop H. 1974 Genus IV. Acinetobacter Brisou and Prévôt 1954. 436–438 Buchanan R. E., Gibbons N. E.ed Bergey’s manual of determinative bacteriology, 8th ed.. The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  21. Morton D. J., Barrett E. L. 1982; Gram-negative respiratory bacteria which cause ropy milk constitute a distinct cluster within the genus Acinetobacter. Curr. Microbiol. 7:107–112
    [Google Scholar]
  22. Pagel J. E., Seyfried P. L. 1976; Numerical taxonomy of aquatic Acinetobacter isolates. J. Gen. Microbiol. 95:220–232
    [Google Scholar]
  23. Pinter M., De Ley J. 1969; Overall similarity and DNA base composition of some Acinetobacter strains. Antonie van Leeuwenhoek J. Microbiol. Serol. 35:209–214
    [Google Scholar]
  24. Richard C. 1972; Méthodes rapides pour l’étude des réactions de rouge de méthyle et Voges-Proskauer. Ann. Inst. Pasteur (Paris) 122:979–986
    [Google Scholar]
  25. Rocourt J., Grimont F., Grimont P. A. D., Seeliger H. P. R. 1982; DNA relatedness among serovars of Listeria monocytogenes sensu lato. Curr. Microbiol. 7:383–388
    [Google Scholar]
  26. Skerman V. B. D., McGowan V., Sneath P. H. A.ed 1980 Approved lists of bacterial names. Int. J. Syst. Bacteriol. 30:225–420
    [Google Scholar]
  27. Stenzel W., Mannheim W. 1963; On the classification and nomenclature of some nonmotile and coccoid diplobacteria, exhibiting the properties of Achromobacteriaceae. Int. Bull. Bacteriol. Nomenci. Taxon. 13:195–200
    [Google Scholar]
  28. Véron M. 1975; Nutrition et taxonomie des entérobactéries. I. Méthode d’étude des auxanogrammes. Ann. Microbiol. (Paris) 126A:267–274
    [Google Scholar]
  29. Woese C. R., Weisburg W. G., Hahn C. M., Paster B. J., Zablen L. B., Lewis B. J., Macke T. J., Ludwig W., Stackebrandt E. 1985; The phylogeny of purple bacteria: the gamma subdivision. Syst. Appl. Microbiol. 6:25–33
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-36-2-228
Loading
/content/journal/ijsem/10.1099/00207713-36-2-228
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error