1887

Abstract

The three type strains and a reference strain of examined in this study had a gram-positive type of cell walls. All four strains were susceptible to vancomycin and resistant to colistin, and the overall susceptibility patterns with other antimicrobial agents were consistent with those of gram-positive microorganisms. Strains of were lysed by 3% potassium hydroxide, and strains of were not. However, all of the strains examined had intermediate levels of amebocyte-lysate reactivity that was not lipopolysaccharide associated. Lipopolysaccharide was not detected when whole-cell lysates were digested with proteinase K, separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and silver stained. In addition, 2-keto-3-deoxyoctulosonic acid and heptose were not detected by gas chromatography of -methyloxime acetate derivatives of whole-cell carbohydrates. Saponification and gas chromatography of whole-cell fatty acids showed that none of the four strains examined had detectable levels of hydroxylated fatty acids. Hydroxylated fatty acids or aldehyde fatty acids were not detected in acid hydrolysates of crude cell membrane preparations.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-36-2-288
1986-04-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/36/2/ijsem-36-2-288.html?itemId=/content/journal/ijsem/10.1099/00207713-36-2-288&mimeType=html&fmt=ahah

References

  1. Baron E. J., Wexler H. M., Finegold S. M. 1984 Biochemical and polyacrylamide gel electrophoretic analyses of vaginosis-associated anaerobic curved rods. 65–69 Mardh P.-A., Taylor-Robinson D.ed Bacterial vaginosis Almqvist and Wiksell International; Stockholm:
    [Google Scholar]
  2. Brade H., Galanos C., Luderitz O. 1983; Isolation of 3-deoxy-D-mannooctulosonic acid disaccharide from Salmonella minnesota rough-form lipopolysaccharide. Eur. J. Biochem. 131:201–203
    [Google Scholar]
  3. Carlone G. M., Valadez M. J., Pickett M. J. 1983; Methods for distinguishing gram-positive from gram-negative bacteria. J. Clin. Microbiol. 16:1157–1159
    [Google Scholar]
  4. Christiansen G., Hansen E., Holst E., Christiansen C., Mardh P.-A. 1984 Genetic relationships of short and long anaerobic curved rods isolated from the vagina. 75–78 Mardh P.-A., Taylor-Robinson D.ed Bacterial vaginosis Almqvist and Wiksell International; Stockholm:
    [Google Scholar]
  5. Curtis A. H. 1913; A motile curved anaerobic bacillus in uterine discharges. J. Infect. Dis. 12:165–169
    [Google Scholar]
  6. Dees S. B., Carlone G. M., Hollis D., Moss C. W. 1985; Chemical and phenotypic characteristics of Flavobacterium thalpophilum compared with those of other Flavobacterium and Sphingobacterium species. Int. J. Syst. Bacteriol. 35:16–22
    [Google Scholar]
  7. Dulley J. R., Greene P. A. 1975; A simple technique for eliminating interference by detergents in the Lowry method of protein determination. Anal. Biochem. 64:136–141
    [Google Scholar]
  8. Durieux R., Dublanchet A. 1980; Les “vibrions” anaérobies des leucorrhées. I. Technique d’isolement et sensibilité aux antibiotiques. Med. Mal. Infect. 10:109–115
    [Google Scholar]
  9. Ezaki T., Suzuki S. 1982; Achromopeptidase for lysis of anaerobic gram-positive cocci. J. Clin. Microbiol. 16:844–846
    [Google Scholar]
  10. Finegold S. M. 1977 Antimicrobial agent susceptibility of anaerobic bacteria. 513–533 Anaerobic bacteria in human disease Academic Press, Inc.; New York:
    [Google Scholar]
  11. Glupczynski Y., Labbe M., Crokaert F., Pepersack F., van Der Auwera P., Yourassowsky E. 1984; Isolation of Mobiluncus in four cases of extragenital infections in adult women. Eur. J. Clin. Microbiol. 3:433–435
    [Google Scholar]
  12. Goldfine H., Panos C. 1971; Phospholipids of Clostridium butyricum. IV. Analysis of the positional isomers of monounsaturated and cyclopropane fatty acids and alk-l-enyl ethers by capillary column chromatography. J. Lipid Res. 12:214–220
    [Google Scholar]
  13. Gregersen T. 1978; Rapid method for distinction of gramnegative from gram-positive bacteria. Eur. J. Appl. Microbiol. Biotechnol. 5:123–127
    [Google Scholar]
  14. Guerrant G. O., Moss C. W. 1984; Determination of monosaccharides as aldononitrile, (9-methyloxime, alditol, and cyclitol acetate derivatives by gas chromatography. Anal. Chern. 56:633–638
    [Google Scholar]
  15. Hammann R., Kronibus A., Viebahn A., Bandis H. 1984; Falcivibrio grandis gen. nov. sp. nov., and Falcivibrio vaginalis gen. nov. sp. nov., a new genus and species to accommodate anaerobic motile curved rods formerly described as “Vibrio mulieris” (Prevot 1940) Breed et al. 1948. Syst. Appl. Microbiol. 5:81–96
    [Google Scholar]
  16. Hitchcock P. J., Brown T. M. 1983; Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels. J. Bacteriol. 154:269–277
    [Google Scholar]
  17. Hjelm E., Hallen A., Forsum U., Wallin J. 1982; Motile anaerobic curved rods in non-specific vaginitis. Eur. J. Sex. Transm. Dis. 1:9–14
    [Google Scholar]
  18. Holdeman L. V., Kelly R. W., Moore W. E. C. 1984 Anaerobic Gram-negative straight, curved and helical rods. 602–662 Krieg N. R., Holt J. G.ed Bergey’s manual of systematic bacteriology The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  19. Jantzen E., Hofstad T. 1981; Fatty acids of Fusobacterium species: taxonomic implications. J. Gen. Microbiol. 123:163–171
    [Google Scholar]
  20. Johnson J. L. 1981 Genetic characterization. 450–472 Gerhardt P., Murray R. G. E., Costilow R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. B.ed Manual of methods for general bacteriology American Society for Microbiology; Washington, D.C:
    [Google Scholar]
  21. Jorgensen J. H., Smith R. F. 1974; Measurement of bound and free endotoxin by the Limulus assay. Proc. Soc. Exp. Biol. Med. 146:1024–1031
    [Google Scholar]
  22. Karkhanis Y. D., Zeltner J. Y., Jackson J. J., Carlo D. J. 1978; A new and improved microassay to determine 2-keto-3-deoxyoctonate in lipopolysaccharide of Gram-negative bacteria. Anal. Biochem. 85:595–601
    [Google Scholar]
  23. Kessler R. E. 1983; Structural requirements for initiation of Limulus amebocyte lysate gelation by lipoteichoic acids. FEMS Microbiol. Lett. 20:343–346
    [Google Scholar]
  24. Kronig I. 1895; Uber die Natur der Scheidenheme, specielle uber das vorkommen anaerober Streptokokken in Scheidensekret Schwangerer. Zentralbl. Gynaekol. 19:409–412
    [Google Scholar]
  25. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227:680–685
    [Google Scholar]
  26. Lechevalier H. 1977; Lipids in bacterial taxonomy–a taxonomist’s view. Crit. Rev. Microbiol. 5:109–210
    [Google Scholar]
  27. Mardh P.-A., Holst E., Moller B. R. 1984 The grivet monkey as a model for study of vaginitis. 201–205 Mardh P.-A., Taylor-Robinson D.ed Bacterial vaginosis Almqvist and Weksell International; Stockholm:
    [Google Scholar]
  28. Masaki T., Tanabe M., Nakamura K., Soejima M. 1981; Studies on a new proteolytic enzyme from Achromobacter lyticus M497-1. I. Purification and some enzymatic properties. Biochim. Biophys. Acta 660:44–50
    [Google Scholar]
  29. Moi H., Danielsson D. 1984 Studies on rabbit hyperimmune, patient and blood donor serum with regard to bactericidal activity and serum antibodies against anaerobic curved rods from patients with bacterial vaginosis. 87–91 Mardh P.-A., Taylor-Robinson D.ed Bacterial vaginosis Almqvist and Wiksell International; Stockholm:
    [Google Scholar]
  30. Moore B. 1954; Observations on a group of anaerobic vaginal vibrios. J. Pathol. Bacteriol. 67:461–473
    [Google Scholar]
  31. National Committee for Clinical Laboratory Standards 1983 Standard methods for dilution antimicrobial susceptibility tests for bacteria which grow aerobically. Tentative Standard M7-T National Committee for Clinical Laboratory Standards; Villanova, Pa:
    [Google Scholar]
  32. Pàhlson C., Forsum U. 1985; Rapid detection of Mobiluncus species. Lancet i:927
    [Google Scholar]
  33. Pearson F. C., Bohon J., Lee W., Bruszer G., Sagona M., Jakubowski G., Dawe R., Morrison D., Dinarello C. 1984; Characterization of Limulus amebocyte lysate-reactive material from hollow-fiber dialyzers. Appl. Environ. Microbiol. 48:1189–1196
    [Google Scholar]
  34. Pearson F. C., Weary M. 1980; The Limulus amebocyte lysate test for endotoxin. BioScience 30:461–464
    [Google Scholar]
  35. Peloux Y., Thomas P. 1981; A propos de quelques bactéries mobiles anaérobies gram negatives. Rev. Inst. Pasteur Lyon 14:103–111
    [Google Scholar]
  36. Prévôt A. R. 1940 Manuel de classification et de détermination des bactéries anaérobies. Masson et Cie; Paris:
    [Google Scholar]
  37. Roberts M. C., Hillier S. L., Schoenknecht F. D., Holmes K. K. 1984; Nitrocellulose filter blots for species identification of Mobiluncus curtisii and Mobiluncus mulieris. J. Clin. Microbiol. 20:826–827
    [Google Scholar]
  38. Silver S. 1980 Antibiotics and chemicals. 103–108 Anaerobic bacteriology for the clinical laboratory C. V. Mosby Co.; St. Louis, Mo:
    [Google Scholar]
  39. Skarin A., Larsson L., Holst E., Mardh P.-A. 1982; Gas chromatographic study of cellular fatty acids of comma-shaped bacteria isolated from the vagina. Eur. J. Clin. Microbiol. 1:307–309
    [Google Scholar]
  40. Skarin A., Larsson L., Holst E., Mardh P.-A. 1984 Gas chromatographic analysis of cellular fatty acids in anaerobic curved bacteria isolated from the vagina. 71–74 Mardh P.-A., Taylor-Robinson D.ed Bacterial vaginosis Almqvist and Wiksell International; Stockholm:
    [Google Scholar]
  41. Skarin A., Weibull C., Mardh P.-A. 1984 Light and electron microscope studies of anaerobic curved bacteria isolated from the vagina. 59–64 Mardh P.-A., Taylor-Robinson D.ed Bacterial vaginosis Almqvist and Wiksell International; Stockholm:
    [Google Scholar]
  42. Spiegel C. A., Eschenbach D. A., Amsel R., Holmes K. K. 1983; Curved anaerobic bacteria in bacterial (nonspecific) vaginosis and their response to antimicrobial therapy. J. Infect. Dis. 148:817–822
    [Google Scholar]
  43. Spiegel C. A., Roberts M. 1984; Mobiluncus gen. nov., Mobiluncus curtisii subsp. curtisii sp. nov., Mobiluncus curtisii subsp. holmesii subsp. nov., and Mobiluncus mulieris sp. nov., curved rods from the human vagina. Int. J. Syst. Bacteriol. 34:177–184
    [Google Scholar]
  44. Sprott M. S., Ingham H. R., Pattman R. S., Clarkson L. M., Codd A. A., Narang H. K. 1984 Motile curved bacilli: isolation and investigation. 107–111 Mardh P.-A., Taylor-Robinson D.ed Bacterial vaginosis Almqvist and Wiksell International; Stockholm:
    [Google Scholar]
  45. Sprott M. S., Ingham H. R., Pattman R. S., Eisenstadt R. L., Short G. R., Narang H. K., Sisson P. R., Selkon J. B. 1983; Characteristics of motile curved rods in vaginal secretions. J. Med. Microbiol. 16:175–182
    [Google Scholar]
  46. Sturm A. W., Sikkenk P. J. H. 1984; Anaerobic curved rods in breast abscess. Lancet ii:1216
    [Google Scholar]
  47. Sullivan J. D. Jr., Valois F. W., Watson S. W. 1976 Endotoxins: the Limulus amebocyte lysate system. 217–236 Bernheimer A. W.ed Mechanisms in bacterial toxinology John Wiley & Sons, Inc.; New York:
    [Google Scholar]
  48. Taylor A. J., Owen R. J. 1984 Morphological and chemical characteristics of anaerobic curved rod-shaped bacteria from the female genital tract. 97–106 Mardh P.-A., Taylor-Robinson D.ed Bacterial vaginosis Almqvist and Wiksell International; Stockholm:
    [Google Scholar]
  49. Thomason J. L., Schreckenberger P. C., Spellacy W. N., Riff L. J., LeBeau L. J. 1984; Clinical and microbiological characterization of patients with nonspecific vaginosis associated with motile, curved anaerobic rods. J. Infect. Dis. 149:801–809
    [Google Scholar]
  50. von Graevenitz A., Bucher C. 1983; Accuracy of the KOH and vancomycin tests in determining the gram-reaction of non-enterobacterial rods. J. Clin. Microbiol. 18:983–985
    [Google Scholar]
  51. Yin E. T., Galanos C., Kinsky S., Bradshaw R. A., Wessler S., Luderitz O., Sarmiento M. E. 1972; Picogram-sensitive assay for endotoxin: gelation of Limulus polyphemus blood cell lysate induced by purified lipopolysaccharide and lipid A from gram-negative bacteria. Biochim. Biophys. Acta 261:284–289
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-36-2-288
Loading
/content/journal/ijsem/10.1099/00207713-36-2-288
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error