@article{mbs:/content/journal/ijsem/10.1099/00207713-36-3-380, author = "WORAKIT, SUCHADA and BOONE, DAVID R. and MAH, ROBERT A. and ABDEL-SAMIE, MOHIY-ELDIN and EL-HALWAGI, M. M.", title = "Methanobacterium alcaliphilum sp. nov., an H2-Utilizing Methanogen That Grows at High pH Values", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "1986", volume = "36", number = "3", pages = "380-382", doi = "https://doi.org/10.1099/00207713-36-3-380", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/00207713-36-3-380", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "Four strains of alkaliphilic methanogens (strains WeN1, WeN2, WeN3, and WeN4T [T = type strain]) previously enriched and isolated from sediments of four low-salt, high-pH (pH 8.3 to 9.3) lakes in the Wadi el Natrun of Egypt were further characterized. These organisms were H2-oxidizing, CO2-reducing, rod-shaped bacteria which grew best at high pH values (strains WeN1, WeN2, and WeN4T preferred pH 8.4 and grew at pH values up to 9.9; strain WeN3 grew best at pH 7.8 and grew at pH values up to 9.2). Each strain required sulfide as a sulfur source and was resistant to elevated concentrations (growth rates in the presence of 0.5% Na2S ยท 9H2O were one-third to one-half the maximum observed rates). The guanine-plus-cytosine content of the deoxyribonucleic acid of strain WeN4T was 57 mol%. A new species of methanogen, Methanobacterium alcaliphilum, is described; strain WeN4 (= DSM 3387) is the type strain.", }