1887

Abstract

The objective of this study was to investigate the antigenic profiles of serogroups 1 to 6 and antigenic relatedness among individual serogroups. A reference precipitate pattern which had 71 anodic- and 6 cathodic-migrating antigens was established with a pooled antigen preparation of sonicated cells of serogroups 1 to 6 against purified homologous rabbit antibody. Sixteen antigens made heat stable by boiling for 10 min were found, of which one could be demonstrated as being the common antigen of gram-negative bacteria and another could be shown as being the serogroup-specific antigen of . Precipitin patterns similar in overall appearance and precipitin numbers were demonstrated for each serogroup by testing antigen preparations of individual serogroups against reference system antibody. Crossed-line experiments revealed < 5 non-cross-reacting antigens among serogroups, with resulting matching coefficients (ratio of the number of cross-reactive to total antigens) all above 0.91. The matching coefficients found when testing antigen preparations from serogroups 7 and 8 were 0.93 and 0.99, respectively. By contrast, the matching coefficients of , and was 0.39, 0.45, 0.39, and 0.41, respectively. Comparison of precipitate patterns and experimental results between the previously described monovalent serogroup 1 system and the polyvalent crossed immunoelectrophoresis system with serogroups 1 to 6 substantiated the antigenic homogencity of and the significant difference in antigenic composition between this taxon and other members of the family . In addition, reproducibility of the antigenic profile of by crossed immunoelectrophoresis was demonstrated.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-36-3-399
1986-07-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/36/3/ijsem-36-3-399.html?itemId=/content/journal/ijsem/10.1099/00207713-36-3-399&mimeType=html&fmt=ahah

References

  1. Axelsen N. H. 1973; Quantitative immunoelectrophoretic methods as tools for a polyvalent approach to standardization in the immunochemistry of Candida albicans. Infect. Immun. 7:949–960
    [Google Scholar]
  2. Barbaree J. M., Sanchez A., Sanden G. N. 1983; Tolerance of Legionella species to sodium chloride. Curr. Microbiol. 9:1–15
    [Google Scholar]
  3. Berdal B. P., Hushovd O., Olsvik O., Odegard O. R., Bergan T. 1982; Demonstration of extracellular proteolytic enzymes from Legionella species strains using synthetic chromogenic peptide substrates. Acta Pathol. Microbiol. Immunol. Scand. Sect. B 90:119–123
    [Google Scholar]
  4. Brenner D. J. 1984 Classification of Legionellaceae. 55–60 Thornsberry C., Balows A., Feeley J. C., Jakubowski W.ed Legionella. Proceedings of the 2nd International Symposium American Society for Microbiology; Washington, D.C:
    [Google Scholar]
  5. Brenner D. J., Steigerwalt A. G., Gorman G. W., Wilkinson H. W., Bibb W. F., Hackel M., Tyndall R. L., Campbell J., Feeley J. C., Thacker W. L., Skaliy P., Martin W. T., Brake B. J., Fields B. S., McEachern H. V., Corcoran L. K. 1985; Ten new species of Legionella. Int. J. Syst. Bacteriol. 35:50–59
    [Google Scholar]
  6. Brenner D. J., Steigerwalt A. G., McDade J. E. 1979; Classification of the Legionnaires’ disease bacterium: Legionella pneumophila, genus novum, species nova, of the family Legionellaceae familia nova. Ann. Intern. Med. 90:656–658
    [Google Scholar]
  7. Chaparas S. D., Lind A., Ouchterlony Ô., Ridell M. 1983; Terminology guidelines for serotaxonomic studies using immunodiffusion and immunoelectrophoresis. Int. J. Syst. Bacteriol. 33:414–416
    [Google Scholar]
  8. Cherry W. B., Pittman B., Harris P. P., Hebert G. A., Thomason B. M., Thacker L., Weaver R. E. 1978; Detection of Legionnaires disease bacteria by direct immunofluorescent staining. J. Clin. Microbiol. 8:329–338
    [Google Scholar]
  9. Collins M. T., Cho S.-N., Hoiby N., Espersen F., Baek L., Reif J. S. 1983; Crossed immunoelectrophoretic analysis of Legionella pneumophila serogroup 1 antigens. Infect. Immun. 39:1428–1440
    [Google Scholar]
  10. Collins M. T., Espersen F., Hpiby N., Cho S.-N., Friis-Moiler A., Reif J. S. 1983; Cross-reactions between Legionella pneumophila (serogroup 1) and twenty-eight other bacterial species, including other members of the family Legionellaceae. Infect. Immun. 39:1441–1456
    [Google Scholar]
  11. Ehret W., Ruckdeschel G. 1983; Species specific membrane proteins of Legionellaceae. Zentralbl. Bakteriol. Abt. 1 Grig. A 255:33–38
    [Google Scholar]
  12. Espersen F., Hertz J. B., Hpiby N. 1980; Cross-reactions between Salmonella typhi antigens and of 24 other bacterial species. Acta Pathol. Microbiol. Scand. Sect. B 88:243–248
    [Google Scholar]
  13. Espersen F., Hertz J. B., Hoiby N. 1981; Cross-reactions between Staphylococcus epidermidis and 23 other bacterial species. Acta Pathol. Microbiol. Scand. Sect. B 89:253–260
    [Google Scholar]
  14. Fox A., Lau P. Y., Brown A., Morgan S. L., Zhu Z.-T., Lerna M., Walla M. D. 1984; Capillary gas chromatographic analysis of carbohydrates of Legionella pneumophila and other members of the family Legionellaceae. J. Clin. Microbiol. 19:326–332
    [Google Scholar]
  15. Franzus M. J., Malcolm G. B., Pine L. 1984 Taxonomic evaluation of amino acid metabolism by Legionella species. 74–77 Thornsberry C., Balows A., Feeley J. C., Jakubowski W.ed Legionella. Proceedings of the 2nd International Symposium American Society for Microbiology; Washington, D.C:
    [Google Scholar]
  16. Garrity G. M., Brown A., Vickers R. M. 1980; Tatlockia and Fluoribacter: two new genera of organisms resembling Legionella pneumophila. Int. J. Syst. Bacteriol. 30:609–614
    [Google Scholar]
  17. Groothuis D. G., Veenendaal H. R., Meenhorst P. L. 1984 Serogrouping of legionellae by slide agglutination test with monospecific sera. 35–37 Thornsberry C., Balows A., Feeley J. C., Jakubowski W.ed Legionella. Proceedings of the 2nd International Symposium American Society for Microbiology; Washington, D.C:
    [Google Scholar]
  18. Harboe M., Closs O. 1983 Immunization with precipitates obtained by crossed immunoelectrophoresis. 353–359 Axelsen N. H.ed Handbook of Immunoprecipitation-in-gel techniques Blackwell Scientific Publications, Ltd.; Oxford:
    [Google Scholar]
  19. Harboe N. M. G., Ingild A. 1983 Immunization, isolation of immunoglobulins and antibody titer determination. 345–351 Axelsen N. H.ed Handbook of immunoprecipitation-in-gel techniques Blackwell Scientific Publications, Ltd.; Oxford:
    [Google Scholar]
  20. Hébert G. A. 1981; Hippurate hydrolysis by Legionella pneumophila. J. Clin. Microbiol. 13:240–242
    [Google Scholar]
  21. Hoiby N. 1975; The serology of Pseudomonas aeruginosa analyzed by means of quantitative immunoelectrophoretic methods. III. Reproducibility of a polyvalent P. aeruginosa reference standard-antigen. Acta Pathol. Microbiol. Scand. Sect. B 83:433–442
    [Google Scholar]
  22. Hoiby N. 1976; The serology of Pseudomonas aeruginosa analyzed by means of quantitative immunoelectrophoretic methods. IV. Production of polyvalent pools of rabbit antiserum against P. aeruginosa (reference standard-antibody). Acta Pathol. Microbiol. Scand. Sect. B 84:372–382
    [Google Scholar]
  23. Hornok L. 1979; Comparison of Fusarium acuminatum and Fusarium culmorum isolates by means of tandem-crossed immunoelectrophoresis. Antonie van Leeuwenhoek J. Microbiol. 45:293–302
    [Google Scholar]
  24. Johnson W., Pesanti E., Elliott J. 1979; Serospecificity and opsonic activity of antisera to Legionella pneumophila. Infect. Immun. 26:698–704
    [Google Scholar]
  25. Joly J. R., Kenny G. E. 1982; Antigenic analysis of Legionella pneumophila and Tatlockia micdadei (Legionella micdadei) by two-dimension (crossed) immunoelectrophoresis. Infect. Immun. 35:721–729
    [Google Scholar]
  26. Karr D. E., Bibb W. F., Moss C. W. 1982; Isoprenoid quinones of the genus Legionella. J. Clin. Microbiol. 15:1044–1048
    [Google Scholar]
  27. Lema M., Brown A. 1983; Electrophoretic characterization of soluble protein extracts of Legionella pneumophila and other members ofthe family Legionellaceae. J. Clin. Microbiol. 17:1132–1140
    [Google Scholar]
  28. Mayberry W. R. 1984; Monohydroxy and dihydroxy fatty acid composition of Legionella species. Int. J. Syst. Bacteriol. 34:321–326
    [Google Scholar]
  29. Moss C. W., Weaver R. E., Dees S. B., Cherry W. B. 1977; Cellular fatty acid composition of isolates from Legionnaires disease. J. Clin. Microbiol. 6:140–143
    [Google Scholar]
  30. Orrison L. H., Bibb W. F., Cherry W. B., Thacker L. 1983; Determination of antigenic relationships among legionellae and non-legionellae by direct fluorescent-antibody and immunodiffusion tests. J. Clin. Microbiol. 17:332–337
    [Google Scholar]
  31. Pine L., Hoffman P. S., Malcolm G. B., Benson R. F., Keen M. G. 1984; Determination of catalase, peroxidase, and superoxide dismutase within the genus Legionella. J. Clin. Microbiol. 20:421–429
    [Google Scholar]
  32. Schiptz P. P., Hoi N., Hertz J. B. 1979; Cross-reactions between Haemophilus influenzae and nineteen other bacterial species. Acta Pathol. Microbiol. Scand. Sect. B 87:337–344
    [Google Scholar]
  33. Svendsen P. J., Weeke B., Johansson B.-G. 1983 Chemicals, solutions, equipment and general procedures. 3–20 Axelsen N.H.ed Handbook of immunoprecipitation-in-gel techniques Blackwell Scientific Publications, Ltd.; Oxford:
    [Google Scholar]
  34. Thacker W. L., Wilkinson H. W., Benson R. F. 1983; Comparison of slide agglutination test and direct immunofluorescence assay for identification of Legionella isolates. J. Clin. Microbiol. 18:1113–1118
    [Google Scholar]
  35. Thomason B. M., Bibb W. F. 1984; Use of absorbed antisera for demonstration of antigenic variation among strains of Legionella pneumophila serogroup 1. J. Clin. Microbiol. 19:794–797
    [Google Scholar]
  36. Thorpe T. C., Miller R. D. 1981; Extracellular enzymes of Legionella pneumophila. Infect. Immun. 33:632–635
    [Google Scholar]
  37. Vickers R. M., Yu V. L. 1984; Clinical laboratory differentiation of Legionellaceae family members with pigment production and fluorescence on media supplemented with aromatic substrates. J. Clin. Microbiol. 19:583–587
    [Google Scholar]
  38. Walla M. D., Lau P. Y., Morgan S. L. 1984; Capillary gas chromatography-mass spectrometry of carbohydrate components of legionellae and other bacteria. J. Chromatogr. 288:399–413
    [Google Scholar]
  39. Wilkinson H. W., Fikes B. J., Cruce D. D. 1979; Indirect immunofluorescence test for serodiagnosis for Legionnaires disease: evidence for serogroup diversity of Legionnaires disease bacterial antigens and for multiple specificity of human antibodies. J. Clin. Microbiol. 9:379–383
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-36-3-399
Loading
/content/journal/ijsem/10.1099/00207713-36-3-399
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error