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Volume 37, Issue 4

sp. nov. from the Salt Marsh Mosquito † Free

Abstract

Spiroplasma strain AES-1, which was isolated from the salt marsh mosquito , is the monotypic representative of group X. This strain differed in serological reactivity, growth pattern, and morphology from previously described spiroplasmas. Most cells of strain AES-1 were nonhelical; helical cells were about 1 to 2 μm long and rarely had more than one or two turns. Optimal growth occurred at 31°C; no growth was observed at 37°C. Cholesterol and amino acids were required for growth. Strain AES-1 utilized glucose, fructose, mannose, mannitol, and trehalose in the chemically defined CC-494 medium, failed to hydrolyze arginine and urea but reduced tellurite either aerobically or anaerobically, and was susceptible to digitonin. Polyacrylamide gel electrophoretic patterns of the cell proteins of strain AES-1 were distinctive and were characterized by a major low-molecular-weight protein band. The guanine-plus-cytosine content was 26.6 mol% as determined by thermal denaturation. Serological studies, using growth inhibition, deformation, and metabolism inhibition tests, showed that strain AES-1 was distinct from all recognized spiroplasma groups and subgroups, including the six named species; avidin-biotin-peroxidase enzyme-linked immunosorbent assays showed that strain AES-1 was unrelated to , and . Strain AES-1 is the first to be characterized biochemically in a chemically defined medium. We propose that strain AES-1 (ATCC 35112) be designated as the monotypic representative of a new species, sp. nov.

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Copyright © 1987 International Union of Microbiological Societies
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1987-10-01
2024-05-02
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References

  1. Abalain-Colloc M. L., Chastel C., Tully J. G., Bove J. M., Whitcomb R. F., Gilot B., Williamson D. L. 1987; Spiroplasma sabaudiense sp. nov. from mosquitoes collected in France. Int. J. Syst. Bacteriol. 37:260–265
     [Google Scholar]
  2. Aluotto B. B., Wittier R. G., Williams C. O., Faber J. E. 1970; Standardized bacteriologic techniques for the characterization of Mycoplasma species. Int. J. Syst. Bacteriol. 20:35–58
     [Google Scholar]
  3. Archer D. B., Best J. 1980; Serological relatedness of spiroplasmas estimated by enzyme-linked immunosorbent assay and crossed immunoelectrophoresis. J. Gen. Microbiol. 119:413–422
     [Google Scholar]
  4. Bove J. M., Saillard C. 1979 Cell biology of spiroplasmas. 83–153 Whitcomb R. F., Tully J. G.ed The mycoplasmas 3 Academic Press, Inc.; New York:
     [Google Scholar]
  5. Bove J. M., Saillard C., Junca P., Degorce-Dumas J.-R., Ricard B., Nhami A., Whitcomb R. F., Williamson D. L., Tully J. G. 1982; Guanine-plus-cytosine content, hybridization percentages, and EcoRI restriction enzyme profiles of spiroplasmal DNA. Rev. Infect. Dis 4:SupplS129–S136
     [Google Scholar]
  6. Bradbury J. M. 1977; Rapid biochemical tests for characterization of the Mycoplasmatales. J. Clin. Microbiol. 5:531–534
     [Google Scholar]
  7. Carle P., Saillard C., Bove J. M. 1983 Determination of guanine plus cytosine content of DNA. 301–308 Razin S., Tully J. G.ed Methods in mycoplasmology 1 Academic Press, Inc.; New York:
     [Google Scholar]
  8. Chang C. J., Chen T. A. 1982; Spiroplasmas: cultivation in chemically defined medium. Science 215:1121–1122
     [Google Scholar]
  9. Chang C. J., Chen T. A. 1983; Nutritional requirements of two flower spiroplasmas and honeybee spiroplasma. J. Bacteriol. 153:452–457
     [Google Scholar]
  10. Chastel C., Gilot B., LeGoff F., Gruffaz R., Abalain-Colloc M.-L. 1985; Isolement de spiroplasmes en France (Savoie, Alpes du Nord) a partir de moustiques du genre Aedes. C.R. Acad. Sci. Paris Ser. Ill 300:261–266
     [Google Scholar]
  11. Chen C. S., Chen T. A. 1980; Comparison of immunized mouse ascites fluids and rabbit sera in serological tests of two spiroplasmas. Phytopathology 70:279–282
     [Google Scholar]
  12. Clark T. B. 1982; Spiroplasmas: diversity of arthropod reservoirs and host-parasite relationships. Science 217:57–59
     [Google Scholar]
  13. Clark T. B., Whitcomb R. F., Tully J. G., Mouches C., Saillard C., Bove J. M., Wroblewski H., Carle P., Rose D. L., Henegar R. B., Williamson D. L. 1985; Spiroplasma melliferum, a new species from the honeybee (Apis mellifera). Int. J. Syst. Bacteriol. 35:296–308
     [Google Scholar]
  14. Clyde W. A. Jr. 1964; Mycoplasma species identification based upon growth inhibition by specific antisera. J. Immunol. 92:958–965
     [Google Scholar]
  15. Cole R. M., Tully J. G., Popkin T. J., Bove J. M. 1973; Ultrastructure of the agent of citrus “stubborn disease”. J. Bacteriol. 115:367–386
     [Google Scholar]
  16. Crans W. J. 1977; The status of Aedes sollicitans as an epidemic vector of eastern equine encephalitis in New Jersey. Mosquito News 37:85–89
     [Google Scholar]
  17. Crans W. J., Downing J. D., Slaff M. E. 1976; Behavioral changes in the salt marsh mosquito, Aedes sollicitans, as a result of increased physiological age. Mosquito News 36:437–445
     [Google Scholar]
  18. Daniels M. J., Archer D. B., Stephens M. A., Townsend R., Longland J. M., Best J. 1980; Comparison of spiroplasmas by polyacrylamide gel electrophoresis of cell proteins. Curr. Microbiol. 4:377–380
     [Google Scholar]
  19. Davis R. E., Lee I. M. 1982; Comparative properties of spiroplasmas and emerging taxonomic concepts: a proposal. Rev. Infect. Dis. 4:Suppl.S122–S128
     [Google Scholar]
  20. Davis R. E., Lee I. M., Basciano L. K. 1979; Spiroplasmas: serological grouping of strains associated with plants and insects. Can. J. Microbiol. 25:861–866
     [Google Scholar]
  21. Davis R. E., Lee I.-M., Worley J. F. 1981; Spiroplasma floricola, a new species isolated from surfaces of flowers of the tulip tree, Liriodendron tulipifera L. Int. J. Syst. Bacteriol. 31:456–464
     [Google Scholar]
  22. Fabricant J., Freundt E. A. 1967; Importance of extension and standardization of laboratory tests for the identification and classification of mycoplasmas. Ann. N.Y. Acad. Sci. 143:50–58
     [Google Scholar]
  23. Freundt E. A., Andrews B. E., Erno H., Kunze M., Black F. T. 1973; The sensitivity of Mycoplasmatales to sodium-poly-anethol-sulfonate and digitonin. Zentralbl. BakterioL Para-sitenkd. Infektionskr. Hyg. Abt. 1 Orig. Reihe A 225:104–112
     [Google Scholar]
  24. Hsu S. M., Raine L. 1981; Protein A, avidin and biotin in immunochemistry. J. Histochem. Cytochem. 29:1349–1353
     [Google Scholar]
  25. International Committee on Systematic Bacteriology Subcommittee on the Taxonomy of Mollicutes 1979; Proposal of minimal standards for descriptions of new species of the class Mollicutes. Int. J. Syst. Bacteriol. 29:172–180
     [Google Scholar]
  26. Jones A. L., Whitcomb R. F., Williamson D. L., Coan M. E. 1977; Comparative growth and primary isolation of spiroplasmas in media based on insect tissue culture formulations. Phytopathology 67:738–746
     [Google Scholar]
  27. Junca P., Saillard C., Tully J. G., Garcia-Jurado O., Degorce-Dumas J. R., Mouches C., Vignault J. C., Vogel R., McCoy R., Whitcomb R., Williamson D., Latrille J., Bove J. M. 1980; Characterisation de spiroplasmes isoles d’insectes et de fleurs de France continentale, de Corse et du Maroc. Proposition pour une classification des spiroplasmes. C.R. Acad. Sci. Paris Ser. D 290:1209–1212
     [Google Scholar]
  28. Lee I.-M., Davis R. E. 1980; DNA homology among diverse spiroplasma strains representing several serological groups. Can. J. Microbiol. 26:1356–1363
     [Google Scholar]
  29. Liap C. H., Chen T. A. 1977; Culture of corn stunt spiroplasma in a simple medium. Phytopathology 67:802–807
     [Google Scholar]
  30. Liao C. H., Chen T. A. 1981; Deoxyribonucleic acid hybridization between Spiroplasma citri and the corn stunt spiroplasma. Curr. Microbiol. 5:83–86
     [Google Scholar]
  31. Lin C. P., Chen T. A. 1985; Production of monoclonal antibodies against Spiroplasma citri. Phytopathology 75:848–851
     [Google Scholar]
  32. Manchee R. J., Taylor-Robinson D. 1968; Hemadsorption and hemagglutination by mycoplasmas. J. Gen. Microbiol. 50:465–478
     [Google Scholar]
  33. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J. Mol. Biol. 3:208–218
     [Google Scholar]
  34. Mouches C., Bove J. M. 1983 Electrophoretic characterization of mycoplasma proteins. 241–255 Razin S., Tully J. G.ed Methods in mycoplasmology 1 Academic Press, Inc.; New York:
     [Google Scholar]
  35. Mouches C., Bove J. M., Albisetti J., Clark T. B., Tully J. G. 1982; A spiroplasma of serogroup IV causes a Maydisease-like disorder of honeybees in southwestern France. Microb. Ecol. 8:387–399
     [Google Scholar]
  36. Razin S., Tully J. G. 1970; Cholesterol requirement of mycoplasmas. J. Bacteriol. 102:306–310
     [Google Scholar]
  37. Saillard C., Vignault J. C., Bove J. M., Raie A., Tully J. G., Williamson D. L., Fos A., Garnier M., Gadeau A., Carle P., Whitcomb R. F. 1987; Spiroplasma phoeniceum sp. nov., a new plant-pathogenic species from Syria. Int. J. Syst. Bacteriol. 37:106–115
     [Google Scholar]
  38. Schreir M., Kohler G., Hengartner H., Berek C., Trucco M., Forni L., Staehelin T., Stocker J., Takacs B. 1980 Hybridoma techniques. Cold Spring Harbor Laboratory. Cold Spring Harbor; N.Y:
     [Google Scholar]
  39. Shaikh A. A., Johnson W.-E. Jr., Stevens C., Tang A. Y. 1987; Isolation of spiroplasmas from mosquitos in Macon County. Ala. J. Am. Mosq. Control. Assoc. 3:289–295
     [Google Scholar]
  40. Skripal I. G. 1983; Revival of the name Spiroplasmataceae fam. nov., nom. rev., omitted from the 1980 Approved Lists of Bacterial Names. Int. J. Syst. Bacteriol. 33:408
     [Google Scholar]
  41. Slaff M., Chen T. A. 1982; The isolation of a spiroplasma from Aedes sollicitans (Walker) in New Jersey. J. Fla. AntiMosquito Assn. 53:19–21
     [Google Scholar]
  42. Spurr A. R. 1969; A low-viscosity epoxy resin embedding medium for electron microscopy. J. Ultrastruct. Res. 26:31–43
     [Google Scholar]
  43. Townsend R. 1976; Arginine metabolism of Spiroplasma citri. J. Gen. Microbiol. 94:417–420
     [Google Scholar]
  44. Tully J. G., Rose D. L., Clark E., Carle P., Bove J. M., Henegar R. B., Whitcomb R. F., Colflesh D. E., Williamson D. L. 1987; Revised group classification of the genus Spiroplasma (class Mollicutes), with proposed new groups XII to XXIII. Int. J. Syst. Bacteriol. 37:357–364
     [Google Scholar]
  45. Tully J. G., Whitcomb R. F., Bove J. M., Saglio P. 1973; Plant mycoplasmas: serological relation between agents associated with citrus stubborn and corn stunt diseases. Science 182:827–829
     [Google Scholar]
  46. Tully J. G., Whitcomb R. F., Clark H. F., Williamson D. L. 1977; Pathogenic spiroplasmas: cultivation and vertebrate pathogenicity of a new spiroplasma. Science 195:892–894
     [Google Scholar]
  47. Whitcomb R. F. 1983 Culture media for spiroplasmas. 147–158 Razin S., Tully J. G.eds Methods in mycoplasmology 1 Academic Press Inc.; New York:
     [Google Scholar]
  48. Whitcomb R. F., Tully J. G. 1983 The family Spiroplasmataceae and genus Spiroplasma. 781–787 Krieg N. R., Holt J. G.ed Bergey’s manual of systematic bacteriology, 1st. 1 The Williams & Wilkins Co.; Baltimore:
     [Google Scholar]
  49. Whitcomb R. F., Tully J. G., Clark T. B., Williamson D. L., Bove J. M. 1982; Revised serological classification of spiroplasmas, new provisional groups, and recommendations for serotyping of isolates. Curr. Microbiol. 7:291–296
     [Google Scholar]
  50. Whitcomb R. F., Tully J. G., McCawley P., Rose D. L. 1982; Application of the growth inhibition test to Spiroplasma taxonomy. Int. J. Syst. Bacteriol. 32:387–394
     [Google Scholar]
  51. Whitcomb R. F., Tully J. G., Rose D. L., Williamson D. L., Bove J. M. 1983; Serological classification of spiroplasmas: current status. Yale J. Biol. Med. 56:453–459
     [Google Scholar]
  52. Williamson D. L., Tully J. G., Whitcomb R. F. 1979; Serological relationships of spiroplasmas as shown by combined deformation and metabolism inhibition tests. Int. J. Syst. Bacteriol. 29:345–351
     [Google Scholar]
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Spiroplasma culicicola sp. nov. from the Salt Marsh Mosquito Aedes sollicitans†
Int J Syst Evol Microbiol 37, 365 (1987); https://doi.org/10.1099/00207713-37-4-365
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