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Volume 37, Issue 4

Deoxyribonucleic Acid Base Sequence Relatedness among Members of the Yeast Genus Free

Abstract

Deoxyribonucleic acid (DNA) base composition and DNA base sequence relatedness comparisons were used for species delineation in the genus . Base composition values separated the members of the genus into three groups. The groups were further subdivided by comparing base sequences by using DNA/DNA renaturation experiments. Two DNA homology groups were identified. The first group included , and three anamorphs (, and ); the members of this group exhibited ≥90% DNA base sequence complementarity. The second group consisted of , and ; various pairs of these yeasts shared 64 to 98% of their DNA sequences. The two groups were only distantly related to each other (≤15% DNA base sequence complementarity). The other species appear to be unique, not being closely related to either of the two homology groups or to one another. Relationships deduced from comparisons of DNAs agreed well with those deduced by other workers from immunological comparisons of exo-β-glucanases and from isoenzyme analysis but were only in partial agreement with a taxonomic arrangement made on the basis of mating studies. We propose recognition of the following species: , and .

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Copyright © 1987 International Union of Microbiological Societies
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1987-10-01
2024-04-25
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Deoxyribonucleic Acid Base Sequence Relatedness among Members of the Yeast Genus Kluyveromyces
Int J Syst Evol Microbiol 37, 371 (1987); https://doi.org/10.1099/00207713-37-4-371
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