1887

Abstract

To determine deoxyribonucleic acid sequence variability within ribosomal ribonucleic acid (rRNA) genes and flanking regions of , plasmids containing parts of rRNA genes from sp. strain PG50 or type strain PG21 were used as probes to detect restriction fragment length polymorphisms (RFLPs). A total of 26 strains selected to show either maximum diversity (14 strains) or possible cluster formation (12 strains) with respect to antigenic and genetic composition were included in the study. Although most of the RFLPs observed could be explained by variation in restriction enzyme cleavage sites outside the rRNA cistrons, variation in restriction enzyme cleavage sites within rRNA cistrons was also observed. RFLPs within the rRNA genes were demonstrated in three strains from the group selected to show heterogeneity. RFLPs outside the rRNA genes were equally pronounced in the two groups of strains.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-38-1-108
1988-01-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/38/1/ijsem-38-1-108.html?itemId=/content/journal/ijsem/10.1099/00207713-38-1-108&mimeType=html&fmt=ahah

References

  1. Amikam D., Glaser G., Razin S. 1984; Mycoplasmas (Mollicutes) have a low number of rRNA genes. J. Bacteriol. 158:376–378
    [Google Scholar]
  2. Andersen H., Birkelund S., Christiansen G., Freundt E. A. 1987; Electrophoretic analysis of proteins from Mycoplasma hominis strains detected by SDS-PAGE, two-dimensional gel electrophoresis and immunoblotting. J. Gen. Microbiol. 133:181–191
    [Google Scholar]
  3. Barile M. F., Grabowski M. W., Stephens E. B., O’Brien S. J., Simonson J. M., Izumikawa K., Chandler D. K. F., Taylor-Robinson D., Tully J. G. 1983; Mycoplasma hominis-tissue cell interactions: a review with new observations on phenotypic and genotypic properties. Sex. Transm. Dis. 10:345–354
    [Google Scholar]
  4. Boros L, Kiss A., Venetianer P. 1979; Physical map of the seven ribosomal RNA genes of Escherichia coli. Nucleic Acids Res. 6:1817–1830
    [Google Scholar]
  5. Brosius J., Ullrich A., Raker M. A., Gray A., Dull T. J., Gutell R. R., Noller H. F. 1981; Construction and fine mapping of recombinant plasmids containing the rrriB ribosomal RNA operon of E. coli. Plasmid 6:112–118
    [Google Scholar]
  6. Christiansen C., Christiansen G., Rasmussen O. F. 1987; Heterogeneity of M. hominis as detected by a probe for atp genes. Isr. J. Med. Sci. 23:591–594
    [Google Scholar]
  7. Christiansen C., Frydenberg J., Christiansen G., Andersen H., Hedegaard L. 1984; Analysis of the Mycoplasma genome by recombinant DNA technology. Isr. J. Med. Sci. 20:781–784
    [Google Scholar]
  8. Christiansen G., Andersen H., Birkelund S., Freundt E. A. 1987; Genomic and gene variation in Mycoplasma hominis. Isr. J. Med. Sci. 23:595–602
    [Google Scholar]
  9. Freundt E. A., Ernø H., Lemcke R. M. 1979 Identification of Mycoplasmas. 377–434 Bergan T., Norris J. R.ed Methods in microbiology 13 Academic Press, Inc.; London:
    [Google Scholar]
  10. Frydenberg J., Christiansen C. 1985; The sequence of 16S rRNA from Mycoplasma strain PG50. DNA 4:127–137
    [Google Scholar]
  11. Glaser G., Amikam D., Razin S. 1984; Physical mapping of the ribosomal RNA genes of Mycoplasma capricolum. Nucleic Acids Res. 12:2421–2426
    [Google Scholar]
  12. Göbel U., Butler G. H., Stanbridge E. J. 1984; Comparative analysis of Mycoplasma ribosomal RNA operons. Isr. J. Med. Sci. 20:762–764
    [Google Scholar]
  13. Göbel U. B., Geiser A., Stanbridge E. J. 1987; Oligonucleotide probes complementary to variable regions of ribosomal RNA discriminate between Mycoplasma species. J. Gen. Microbiol. 133:1969–1974
    [Google Scholar]
  14. Göbel U. B., Stanbridge E. J. 1984; Cloned Mycoplasma ribosomal RNA genes for the detection of Mycoplasma contamination in tissue cultures. Science 226:1211–1213
    [Google Scholar]
  15. Iwami M., Muto A., Yamao F., Osawa S. 1984; Nucleotide sequence of rrnB 16S ribosomal RNA gene from Mycoplasma capricolum. Mol. Gen. Genet. 196:317–322
    [Google Scholar]
  16. Lin J.-S., Kass E. H. 1974; Serological reactions of Mycoplasma hominis: differences among mycoplasmacidal, metabolic inhibition, and growth agglutination tests. Infect. Immun. 10:535–540
    [Google Scholar]
  17. Lindal L., Zengel J. M. 1986; Ribosomal genes in Escherichia coli. Annu. Rev. Genet. 20:297–326
    [Google Scholar]
  18. Rasmussen O. F., Christiansen C. 1987; Organization of the regions flanking the rRNA cistrons of Mycoplasma strain P650. Isr. J. Med. Sci. 23:342–346
    [Google Scholar]
  19. Razin S. 1985; Molecular biology and genetics of Mycoplasmas (Mollicutes). Microbiol. Rev. 49:419–455
    [Google Scholar]
  20. Razin S., Amikam D., Glaser G. 1984; Mycoplasmal ribosomal RNA genes and their use as probes for detection and identification of Mollicutes. Isr. J. Med. Sci. 20:758–761
    [Google Scholar]
  21. Razin S., Gross M., Wormser M., Pollack Y., Glaser G. 1984; Detection of Mycoplasmas infecting cell cultures by DNA hybridization. In Vitro 20:404–408
    [Google Scholar]
  22. Reich P. R., Somerson N. L., Hybner C. J., Chanock R. M., Weissman S. M. 1966; Genetic differentiation by nucleic acid homology. I. Relationships among mycoplasma species of man. J. Bacteriol. 92:302–310
    [Google Scholar]
  23. Reich P. R., Somerson N. L., Rose J. A., Weissman S. M. 1966; Genetic relatedness among mycoplasmas as determined by nucleic acid homology. J. Bacteriol. 91:153–160
    [Google Scholar]
  24. Somerson N. L., Reich P. R., Walls B. E., Chanock R. M., Weissman S. M. 1966; Genetic differentiation by nucleic acid homology. II. Genotypic variations within two mycoplasma species. J. Bacteriol. 92:311–317
    [Google Scholar]
  25. Southern E. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 78:503–517
    [Google Scholar]
  26. Taylor M. A., Wise K. S., McIntosh M. A. 1985; Selective detection of Mycoplasma hyorhinis using cloned genomic DNA fragments. Infect. Immun. 47:827–830
    [Google Scholar]
  27. Trevino L. B., Haldenwang W. G., Baseman J. B. 1986; Expression of Mycoplasma pneumoniae antigens in Escherichia coli. Infect. Immun. 53:129–134
    [Google Scholar]
  28. Woese C. R. 1987; Bacterial evolution. Microbiol. Rev. 51:221–271
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-38-1-108
Loading
/content/journal/ijsem/10.1099/00207713-38-1-108
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error